P82543 (COXA_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase polypeptide 2A
EC=1.9.3.1

Alternative name(s):
Cytochrome c ba(3) subunit IIA
Cytochrome c oxidase polypeptide IIA
Cytochrome cba3 subunit 2A

Gene names

Name:	cbaD
Ordered Locus Names:	TTHA1133

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	34 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subcellular location	Cell membrane; Single-pass membrane protein.
Mass spectrometry	Molecular mass is 3794.6 Da from positions 1 - 34. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Electron transport Hydrogen ion transport Ion transport Respiratory chain Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
PTM	Formylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 34

34

Cytochrome c oxidase polypeptide 2A

PRO_0000183934

Regions

Transmembrane

4 – 34

31

Helical

Amino acid modifications

Modified residue

1

1

N-formylmethionine

Secondary structure

1

.

.

.

34

Helix Strand Turn

Details...

Helix

6 – 32

27

Sequences

	Sequence		Length	Mass (Da)
	P82543 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: F473EB7D0B179FBB	FASTA	34	3,767
	10 20 30 MEEKPKGALA VILVLTLTIL VFWLGVYAVF FARG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of a novel subunit in ba3-cytochrome oxidase from Thermus thermophilus." Soulimane T., Than M.E., Dewor M., Huber R., Buse G. Protein Sci. 9:2068-2073(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY.
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[3]	"Structure and mechanism of the aberrant ba3-cytochrome c oxidase from Thermus thermophilus." Soulimane T., Buse G., Bourenkov G.P., Bartunik H.D., Huber R., Than M.E. EMBO J. 19:1766-1776(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD70956.1.

RefSeq

YP_144399.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EHK	X-ray	2.40	C	2-34	[»]
1XME	X-ray	2.30	C	1-34	[»]
2QPD	X-ray	3.25	C	1-34	[»]
2QPE	X-ray	2.90	C	1-34	[»]
3BVD	X-ray	3.37	C	1-34	[»]
3EH3	X-ray	3.10	C	2-34	[»]
3EH4	X-ray	2.90	C	2-34	[»]
3EH5	X-ray	2.80	C	2-34	[»]
3QJQ	X-ray	2.90	C	1-34	[»]
3QJR	X-ray	3.20	C	1-34	[»]
3QJS	X-ray	2.80	C	1-34	[»]
3QJT	X-ray	2.95	C	1-34	[»]
3QJU	X-ray	2.90	C	1-34	[»]
3QJV	X-ray	2.80	C	1-34	[»]
3S33	X-ray	4.45	C	2-34	[»]
3S38	X-ray	4.20	C	2-34	[»]
3S39	X-ray	4.80	C	2-34	[»]
3S3A	X-ray	4.25	C	2-34	[»]
3S3B	X-ray	3.30	C	2-34	[»]
3S3C	X-ray	4.00	C	2-34	[»]
3S3D	X-ray	3.75	C	2-34	[»]
3S8F	X-ray	1.80	C	1-34	[»]
3S8G	X-ray	1.80	C	1-34	[»]
4FA7	X-ray	2.50	C	1-34	[»]
4FAA	X-ray	2.80	C	1-34	[»]
4GP4	X-ray	2.80	C	1-34	[»]
4GP5	X-ray	2.70	C	1-34	[»]
4GP8	X-ray	2.80	C	1-34	[»]

ProteinModelPortal

P82543.

SMR

P82543. Positions 2-34.

ModBase

Search...

Protein-protein interaction databases

IntAct

P82543. 1 interaction.

STRING

300852.TTHA1133.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD70956; BAD70956; BAD70956.

GeneID

3168186.

KEGG

ttj:TTHA1133.

PATRIC

23957225. VBITheThe93045_1112.

Phylogenomic databases

ProtClustDB

CLSK738611.

Family and domain databases

InterPro

IPR012538. Cyt_c_oxidase_su2a.
[Graphical view]

Pfam

PF08113. CoxIIa. 1 hit.
[Graphical view]

SUPFAM

SSF81473. CoxIIa. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P82543.

Entry information

Entry name

COXA_THET8

Accession

Primary (citable) accession number: P82543
Secondary accession number(s): Q5SJ81

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program