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Protein

Guanine nucleotide-binding protein G(q) subunit alpha

Gene

Gnaq

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531MagnesiumBy similarity
Metal bindingi186 – 1861MagnesiumBy similarity
Binding sitei331 – 3311GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538GTPBy similarity
Nucleotide bindingi180 – 1867GTPBy similarity
Nucleotide bindingi205 – 2095GTPBy similarity
Nucleotide bindingi274 – 2774GTPBy similarity

GO - Molecular functioni

  • alkylglycerophosphoethanolamine phosphodiesterase activity Source: MGI
  • GTPase activity Source: InterPro
  • GTP binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • protein complex binding Source: RGD
  • signal transducer activity Source: UniProtKB-KW
  • type 2A serotonin receptor binding Source: RGD

GO - Biological processi

  • activation of phospholipase C activity Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of potassium ion transport Source: RGD
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of smooth muscle cell proliferation Source: RGD
  • protein heterotrimerization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(q) subunit alpha
Alternative name(s):
Guanine nucleotide-binding protein alpha-q
Gene namesi
Name:Gnaq
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620770. Gnaq.

Subcellular locationi

  • Nucleus By similarity
  • Membrane By similarity
  • Nucleus membrane By similarity

  • Note: Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes.By similarity

GO - Cellular componenti

  • caveola Source: RGD
  • cytosol Source: RGD
  • heterotrimeric G-protein complex Source: RGD
  • membrane Source: RGD
  • nuclear membrane Source: UniProtKB-SubCell
  • plasma membrane Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Guanine nucleotide-binding protein G(q) subunit alphaPRO_0000203762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi9 – 91S-palmitoyl cysteineBy similarity
Lipidationi10 – 101S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP82471.
PRIDEiP82471.

2D gel databases

World-2DPAGE0004:P82471.

PTM databases

iPTMnetiP82471.
PhosphoSiteiP82471.
SwissPalmiP82471.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Binds SLC9A3R1. Forms a complex with PECAM1 and BDKRB2. Interacts with PECAM1 (By similarity).By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD
  • type 2A serotonin receptor binding Source: RGD

Protein-protein interaction databases

BioGridi249565. 1 interaction.
MINTiMINT-4568304.
STRINGi10116.ENSRNOP00000019174.

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(q) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0085. Eukaryota.
ENOG410XNVQ. LUCA.
HOVERGENiHBG063184.
InParanoidiP82471.
KOiK04634.
PhylomeDBiP82471.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000654. Gprotein_alpha_Q.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00442. GPROTEINAQ.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P82471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLESIMACC LSEEAKEARR INDEIERQLR RDKRDARREL KLLLLGTGES
60 70 80 90 100
GKSTFIKQMR IIHGSGYSDE DKRGFTKLVY QNIFTAMQAM VRAMDTLKIP
110 120 130 140 150
YKYEHNKAHA QLVREVDVEK VSAFENPYVD AIKSLWNDPG IQECYDRRRE
160 170 180 190 200
YQLSDSTKYY LNDLDRVADP SYLPTQQDVL RVRVPTTGII EYPFDLQSVI
210 220 230 240 250
FRMVDVGGQR SERRKWIHCF ENVTSIMFLV ALSEYDQVLV ESDNENRMEE
260 270 280 290 300
SKALFRTIIT YPWFQNSSVI LFLNKKDLLE EKIMYSHLVD YFPEYDGPQR
310 320 330 340 350
DAQAAREFIL KMFVDLNPDS DKIIYSHFTC ATDTENIRFV FAAVKDTILQ

LNLKEYNLV
Length:359
Mass (Da):42,144
Last modified:July 7, 2009 - v2
Checksum:i33569BEE7BFF42BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911V → I (PubMed:1333286).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF234260 mRNA. Translation: AAF59930.1.
L37294 mRNA. Translation: AAB02848.1.
RefSeqiNP_112298.1. NM_031036.1.
UniGeneiRn.22817.

Genome annotation databases

GeneIDi81666.
KEGGirno:81666.
UCSCiRGD:620770. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF234260 mRNA. Translation: AAF59930.1.
L37294 mRNA. Translation: AAB02848.1.
RefSeqiNP_112298.1. NM_031036.1.
UniGeneiRn.22817.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249565. 1 interaction.
MINTiMINT-4568304.
STRINGi10116.ENSRNOP00000019174.

PTM databases

iPTMnetiP82471.
PhosphoSiteiP82471.
SwissPalmiP82471.

2D gel databases

World-2DPAGE0004:P82471.

Proteomic databases

PaxDbiP82471.
PRIDEiP82471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81666.
KEGGirno:81666.
UCSCiRGD:620770. rat.

Organism-specific databases

CTDi2776.
RGDi620770. Gnaq.

Phylogenomic databases

eggNOGiKOG0085. Eukaryota.
ENOG410XNVQ. LUCA.
HOVERGENiHBG063184.
InParanoidiP82471.
KOiK04634.
PhylomeDBiP82471.

Miscellaneous databases

PROiP82471.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000654. Gprotein_alpha_Q.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00442. GPROTEINAQ.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Rat G alpha q subunit."
    Strotmann R.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Signal-transducing G proteins and antidepressant drugs: evidence for modulation of alpha subunit gene expression in rat brain."
    Lesch K.-P., Manji H.K.
    Biol. Psychiatry 32:549-579(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 80-235.
    Tissue: Brain cortex.
  3. "GTP-binding protein expression in glomerular mesangial cells."
    Thomas C.P.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 244-337.
    Strain: Sprague-Dawley.
    Tissue: Kidney cortex.

Entry informationi

Entry nameiGNAQ_RAT
AccessioniPrimary (citable) accession number: P82471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 7, 2009
Last modified: June 8, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.