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Protein

E3 ubiquitin-protein ligase Midline-1

Gene

Mid1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi119Zinc 1By similarity1
Metal bindingi122Zinc 1By similarity1
Metal bindingi134Zinc 2By similarity1
Metal bindingi137Zinc 2By similarity1
Metal bindingi142Zinc 1By similarity1
Metal bindingi145Zinc 1By similarity1
Metal bindingi150Zinc 2By similarity1
Metal bindingi159Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 60RING-typePROSITE-ProRule annotationAdd BLAST51
Zinc fingeri116 – 165B box-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri172 – 212B box-type 2PROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Midline-1 (EC:2.3.2.27)
Alternative name(s):
RING finger protein Midline-1
RING-type E3 ubiquitin transferase Midline-1Curated
Tripartite motif-containing protein 18
Gene namesi
Name:Mid1
Synonyms:Fxy, Trim18
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2640. Mid1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: Microtubule-associated.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562311 – 667E3 ubiquitin-protein ligase Midline-1Add BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei92PhosphoserineBy similarity1
Modified residuei96PhosphoserineBy similarity1
Modified residuei511PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP82458.
PRIDEiP82458.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003613.
GenevisibleiP82458. RN.

Interactioni

Subunit structurei

Homodimer or heterodimer with MID2. Interacts with IGBP1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004873.

Structurei

3D structure databases

ProteinModelPortaliP82458.
SMRiP82458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini320 – 379COSPROSITE-ProRule annotationAdd BLAST60
Domaini381 – 484Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST104
Domaini482 – 659B30.2/SPRYPROSITE-ProRule annotationAdd BLAST178

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili205 – 264Sequence analysisAdd BLAST60

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 60RING-typePROSITE-ProRule annotationAdd BLAST51
Zinc fingeri116 – 165B box-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri172 – 212B box-type 2PROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFU. Eukaryota.
ENOG410Z43Q. LUCA.
GeneTreeiENSGT00760000118878.
HOGENOMiHOG000049193.
HOVERGENiHBG056432.
InParanoidiP82458.
KOiK08285.
OMAiRERAFDS.
OrthoDBiEOG091G02K9.
PhylomeDBiP82458.

Family and domain databases

CDDicd00063. FN3. 1 hit.
cd00162. RING. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR003649. Bbox_C.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR017903. COS_domain.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR027727. MID1.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PANTHERiPTHR24103:SF550. PTHR24103:SF550. 1 hit.
PfamiView protein in Pfam
PF00041. fn3. 1 hit.
PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiView protein in SMART
SM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00060. FN3. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P82458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNEPVES
60 70 80 90 100
INAFQCPTCR HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET
110 120 130 140 150
RRERAFDANT MSSAEKVLCQ FCDQDPAQDA VKTCVTCEVS YCDECLKATH
160 170 180 190 200
PNKKPFTGHR LIEPIPDSHI RGLMCLEHED EKVNMYCVTD DQLICALCKL
210 220 230 240 250
VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL LAKLIQTCQH
260 270 280 290 300
VEVNASRQEA KLTEECDLLI EIIQERRQII GTKIKEGKVM RLRKLAQQIA
310 320 330 340 350
NCKQCIERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI
360 370 380 390 400
PEINLNDTFD TFALDFSREK KLLECLDYLT APNPPTIREE LCTASYDTIT
410 420 430 440 450
VHWTSDDEFS VVSYELQYTI FTGQANVVSL CNSADSWMIV PNIKQNHYTV
460 470 480 490 500
HGLQSGTKYI FMVKAINQAG SRSSEPGKLK TNSQPFKLDP KSAHRKLKVS
510 520 530 540 550
HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR HYWEVVISGS
560 570 580 590 600
TWYAIGLAYK SAPKHEWIGK NSASWALCRC NNNWVVRHNS KEIPIEPAPH
610 620 630 640 650
PRRVGILLDY DNGSIAFYDA LNSIHLYTFD VALAQPVCPT FTVWNKCLTI
660
ITGLPIPDHL DCTEQLP
Length:667
Mass (Da):75,211
Last modified:June 1, 2000 - v1
Checksum:iBA73528FEAE59603
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186461 mRNA. Translation: AAD56247.1.
RefSeqiNP_075216.1. NM_022927.1.
XP_006256887.1. XM_006256825.3.
XP_017457631.1. XM_017602142.1.
XP_017457632.1. XM_017602143.1.
UniGeneiRn.228943.

Genome annotation databases

EnsembliENSRNOT00000004873; ENSRNOP00000004873; ENSRNOG00000003613.
ENSRNOT00000086414; ENSRNOP00000072598; ENSRNOG00000003613.
GeneIDi54252.
KEGGirno:54252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186461 mRNA. Translation: AAD56247.1.
RefSeqiNP_075216.1. NM_022927.1.
XP_006256887.1. XM_006256825.3.
XP_017457631.1. XM_017602142.1.
XP_017457632.1. XM_017602143.1.
UniGeneiRn.228943.

3D structure databases

ProteinModelPortaliP82458.
SMRiP82458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004873.

Proteomic databases

PaxDbiP82458.
PRIDEiP82458.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004873; ENSRNOP00000004873; ENSRNOG00000003613.
ENSRNOT00000086414; ENSRNOP00000072598; ENSRNOG00000003613.
GeneIDi54252.
KEGGirno:54252.

Organism-specific databases

CTDi4281.
RGDi2640. Mid1.

Phylogenomic databases

eggNOGiENOG410ITFU. Eukaryota.
ENOG410Z43Q. LUCA.
GeneTreeiENSGT00760000118878.
HOGENOMiHOG000049193.
HOVERGENiHBG056432.
InParanoidiP82458.
KOiK08285.
OMAiRERAFDS.
OrthoDBiEOG091G02K9.
PhylomeDBiP82458.

Miscellaneous databases

PROiPR:P82458.

Gene expression databases

BgeeiENSRNOG00000003613.
GenevisibleiP82458. RN.

Family and domain databases

CDDicd00063. FN3. 1 hit.
cd00162. RING. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR003649. Bbox_C.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR017903. COS_domain.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR027727. MID1.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PANTHERiPTHR24103:SF550. PTHR24103:SF550. 1 hit.
PfamiView protein in Pfam
PF00041. fn3. 1 hit.
PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiView protein in SMART
SM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00060. FN3. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRI18_RAT
AccessioniPrimary (citable) accession number: P82458
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: June 1, 2000
Last modified: May 10, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.