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P82458 (TRI18_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Midline-1
Alternative name(s):
Tripartite motif-containing protein 18
Gene names
Name:Mid1
Synonyms:Fxy, Trim18
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length667 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination By similarity.

Subunit structure

Homodimer or heterodimer with MID2. Interacts with IGBP1 By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Note: Microtubule-associated By similarity.

Sequence similarities

Belongs to the TRIM/RBCC family.

Contains 2 B box-type zinc fingers.

Contains 1 B30.2/SPRY domain.

Contains 1 COS domain.

Contains 1 fibronectin type-III domain.

Contains 1 RING-type zinc finger.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
   DomainCoiled coil
Repeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionLigase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpositive regulation of stress-activated MAPK cascade

Inferred from electronic annotation. Source: Compara

   Cellular_componentcytoplasmic microtubule

Inferred from electronic annotation. Source: Compara

   Molecular_functionligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 667667Midline-1
PRO_0000056231

Regions

Domain320 – 37960COS
Domain380 – 481102Fibronectin type-III
Domain482 – 659178B30.2/SPRY
Zinc finger10 – 6051RING-type
Zinc finger116 – 16550B box-type 1
Zinc finger172 – 21241B box-type 2
Coiled coil205 – 26460 Potential

Sites

Metal binding1191Zinc 1 By similarity
Metal binding1221Zinc 1 By similarity
Metal binding1341Zinc 2 By similarity
Metal binding1371Zinc 2 By similarity
Metal binding1421Zinc 1 By similarity
Metal binding1451Zinc 1 By similarity
Metal binding1501Zinc 2 By similarity
Metal binding1591Zinc 2 By similarity

Amino acid modifications

Modified residue921Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P82458 [UniParc].

Last modified June 1, 2000. Version 1.
Checksum: BA73528FEAE59603

FASTA66775,211
        10         20         30         40         50         60 
METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNEPVES INAFQCPTCR 

        70         80         90        100        110        120 
HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET RRERAFDANT MSSAEKVLCQ 

       130        140        150        160        170        180 
FCDQDPAQDA VKTCVTCEVS YCDECLKATH PNKKPFTGHR LIEPIPDSHI RGLMCLEHED 

       190        200        210        220        230        240 
EKVNMYCVTD DQLICALCKL VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL 

       250        260        270        280        290        300 
LAKLIQTCQH VEVNASRQEA KLTEECDLLI EIIQERRQII GTKIKEGKVM RLRKLAQQIA 

       310        320        330        340        350        360 
NCKQCIERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI PEINLNDTFD 

       370        380        390        400        410        420 
TFALDFSREK KLLECLDYLT APNPPTIREE LCTASYDTIT VHWTSDDEFS VVSYELQYTI 

       430        440        450        460        470        480 
FTGQANVVSL CNSADSWMIV PNIKQNHYTV HGLQSGTKYI FMVKAINQAG SRSSEPGKLK 

       490        500        510        520        530        540 
TNSQPFKLDP KSAHRKLKVS HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR 

       550        560        570        580        590        600 
HYWEVVISGS TWYAIGLAYK SAPKHEWIGK NSASWALCRC NNNWVVRHNS KEIPIEPAPH 

       610        620        630        640        650        660 
PRRVGILLDY DNGSIAFYDA LNSIHLYTFD VALAQPVCPT FTVWNKCLTI ITGLPIPDHL 


DCTEQLP

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References

[1]"Evolutionary rate of a gene affected by chromosomal position."
Perry J., Ashworth A.
Curr. Biol. 9:987-989(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF186461 mRNA. Translation: AAD56247.1.
IPIIPI00231578.
RefSeqNP_075216.1. NM_022927.1.
UniGeneRn.228943.

3D structure databases

ProteinModelPortalP82458.
SMRP82458. Positions 87-214, 376-493.
ModBaseSearch...

Proteomic databases

PaxDbP82458.
PRIDEP82458.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000004873; ENSRNOP00000004873; ENSRNOG00000003613.
GeneID54252.
KEGGrno:54252.

Organism-specific databases

CTD4281.
RGD2640. Mid1.

Phylogenomic databases

eggNOGNOG253961.
GeneTreeENSGT00690000101726.
HOGENOMHOG000049193.
HOVERGENHBG056432.
KOK08285.
OrthoDBEOG49S65S.

Gene expression databases

ArrayExpressP82458.
GenevestigatorP82458.
GermOnlineENSRNOG00000003613. Rattus norvegicus.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
InterProIPR001870. B30.2/SPRY.
IPR003649. Bbox_C.
IPR003879. Butyrophylin.
IPR008985. ConA-like_lec_gl_sf.
IPR017903. COS_domain.
IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR006574. PRY.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamPF00041. fn3. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
PRINTSPR01407. BUTYPHLNCDUF.
SMARTSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00060. FN3. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 1 hit.
SSF49265. FN_III-like. 1 hit.
PROSITEPS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio610756.

Entry information

Entry nameTRI18_RAT
AccessionPrimary (citable) accession number: P82458
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: June 1, 2000
Last modified: April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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