P82350 (SGCA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-sarcoglycan
Short name=Alpha-SG

Alternative name(s):
50 kDa dystrophin-associated glycoprotein
Short name=50DAG
Adhalin

Gene names

Name:

Sgca

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Subunit structure	Cross-link to form 2 major subcomplexes: one consisting of SGCB, SGCD and SGCG and the other consisting of SGCB and SGCD. The association between SGCB and SGCG is particularly strong while SGCA is loosely associated with the other sarcoglycans. Interacts with the syntrophin SNTA1. Ref.5 Ref.6
Subcellular location	Cell membrane › sarcolemma; Single-pass type I membrane protein. Cytoplasm › cytoskeleton Ref.6.
Tissue specificity	Striated muscle, both skeletal and cardiac. Ref.1
Developmental stage	In the embryo, expression begins at day 14 and coincides with the onset of primary myogenesis. Ref.1
Sequence similarities	Belongs to the sarcoglycan alpha/epsilon family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular membrane organization Traceable author statement PubMed 12189167. Source: MGI
Cellular_component	cell-cell junction Inferred from direct assay PubMed 19531352. Source: MGI cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Traceable author statement Ref.1. Source: MGI membrane raft Inferred from direct assay PubMed 11259414. Source: MGI sarcoglycan complex Inferred from electronic annotation. Source: InterPro sarcolemma Inferred from direct assay PubMed 11115849PubMed 11259414PubMed 19531352. Source: MGI
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 387	364	Alpha-sarcoglycan		PRO_0000031675
Regions
	Topological domain	24 – 293	270	Extracellular Potential
	Transmembrane	294 – 314	21	Helical; Potential
	Topological domain	315 – 387	73	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	246	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	19	1	R → K in AAB70754. Ref.1
	Sequence conflict	76	1	L → V in AAB70754. Ref.1
	Sequence conflict	149 – 150	2	LP → VS in AAB70754. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P82350 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7C3D98A853D04591	FASTA	387	43,287
	10 20 30 40 50 60 MAAAVTWIPL LAGLLAGLRD TKAQQTTLHL LVGRVFVHPL EHATFLRLPE HVAVPPTVRL 70 80 90 100 110 120 TYHAHLQGHP DLPRWLHYTQ RSPYNPGFLY GSPTPEDRGY QVIEVTAYNR DSFDTTRQRL 130 140 150 160 170 180 LLLIGDPEGP RLPYQAEFLV RSHDVEEVLP TTPANRFLTA LGGLWEPGEL QLLNITSALD 190 200 210 220 230 240 RGGRVPLPIE GRKEGVYIKV GSATPFSTCL KMVASPDSYA RCAQGQPPLL SCYDTLAPHF 250 260 270 280 290 300 RVDWCNVSLV DKSVPEPLDE VPTPGDGILE HDPFFCPPTE ATDRDFLTDA LVTLLVPLLV 310 320 330 340 350 360 ALLLTLLLAY IMCFRREGRL KRDMATSDIQ MFHHCSIHGN TEELRQMAAS REVPRPLSTL 370 380 PMFNVRTGER LPPRVDSAQM PLILDQH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse adhalin: primary structure and expression during late stages of muscle differentiation in vitro." Liu L., Vachon P.H., Kuang W., Xu H., Wewer U.M., Kylsten P., Engvall E. Biochem. Biophys. Res. Commun. 235:227-235(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: FVB/N. Tissue: Myoblast.
[2]	"Developmental expression of sarcoglycan gene products in cultured myocytes." Noguchi S., Wakabayashi E., Imamura M., Yoshida M., Ozawa E. Biochem. Biophys. Res. Commun. 262:88-93(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[3]	"Alpha-sarcoglycan-deficient mice as an animal model for autosomal recessive limb-girdle muscular dystrophy." Duclos F., Straub V., Moore S., Hrstka R., Crosbie R.H., Durbeej M., Lebakken C.S., Holt K.H., Lim L.E., Ettinger A., Sanes J.R., Davidson B.L., Williamson R., Campbell K.P. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 129/Sv.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Tongue.
[5]	"Interactions between dystrophin glycoprotein complex proteins." Madhavan R., Jarrett H.W. Biochemistry 34:12204-12209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SNTA1.
[6]	"Molecular organization of sarcoglycan complex in mouse myotubes in culture." Chan Y.-M., Boennemann C.G., Lidov H.G.W., Kunkel L.M. J. Cell Biol. 143:2033-2044(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019564 mRNA. Translation: AAB70754.1. AB024920 mRNA. Translation: BAA83491.1. AF064081 Genomic DNA. Translation: AAC33447.1. AK075915 mRNA. Translation: BAC36051.1.
IPI	IPI00322911.
PIR	JC5556.
RefSeq	NP_033187.1. NM_009161.4.
UniGene	Mm.18709.
3D structure databases
ProteinModelPortal	P82350.
ModBase	Search...
PTM databases
PhosphoSite	P82350.
Proteomic databases
PRIDE	P82350.
Protocols and materials databases
DNASU	20391.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000100551; ENSMUSP00000098118; ENSMUSG00000001508. ENSMUST00000103162; ENSMUSP00000099451; ENSMUSG00000001508. ENSMUST00000166320; ENSMUSP00000130617; ENSMUSG00000001508.
GeneID	20391.
KEGG	mmu:20391.
UCSC	uc007kzo.2. mouse.
Organism-specific databases
CTD	6442.
MGI	MGI:894698. Sgca.
Phylogenomic databases
eggNOG	NOG323743.
GeneTree	ENSGT00390000005672.
HOGENOM	HOG000074154.
HOVERGEN	HBG006891.
InParanoid	P82350.
KO	K12565.
OMA	HTLDHET.
OrthoDB	EOG490795.
Gene expression databases
ArrayExpress	P82350.
Bgee	P82350.
Genevestigator	P82350.
GermOnline	ENSMUSG00000001508. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR006644. Cadg. IPR015919. Cadherin-like. IPR013783. Ig-like_fold. IPR008908. Sarcoglycan_2. [Graphical view]
PANTHER	PTHR10132. PTHR10132. 1 hit.
Pfam	PF05510. Sarcoglycan_2. 1 hit. [Graphical view]
SMART	SM00736. CADG. 1 hit. [Graphical view]
SUPFAM	SSF49313. Cadherin. 1 hit.
ProtoNet	Search...
Other
NextBio	298330.
SOURCE	Search...

Entry information

Entry name

SGCA_MOUSE

Accession

Primary (citable) accession number: P82350
Secondary accession number(s): O35311, O88490

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program