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Protein

Alpha-sarcoglycan

Gene

Sgca

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. membrane organization Source: MGI
  2. muscle contraction Source: InterPro
  3. muscle organ development Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-sarcoglycan
Short name:
Alpha-SG
Alternative name(s):
50 kDa dystrophin-associated glycoprotein
Short name:
50DAG
Adhalin
Gene namesi
Name:Sgca
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:894698. Sgca.

Subcellular locationi

Cell membranesarcolemma 1 Publication; Single-pass type I membrane protein 1 Publication. Cytoplasmcytoskeleton 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 293270ExtracellularSequence AnalysisAdd
BLAST
Transmembranei294 – 31421HelicalSequence AnalysisAdd
BLAST
Topological domaini315 – 38773CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell-cell junction Source: MGI
  2. cytoplasm Source: UniProtKB-KW
  3. cytoskeleton Source: UniProtKB-SubCell
  4. dystroglycan complex Source: MGI
  5. integral component of plasma membrane Source: MGI
  6. membrane raft Source: MGI
  7. sarcoglycan complex Source: InterPro
  8. sarcolemma Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 387364Alpha-sarcoglycanPRO_0000031675Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP82350.

PTM databases

PhosphoSiteiP82350.

Expressioni

Tissue specificityi

Striated muscle, both skeletal and cardiac.1 Publication

Developmental stagei

In the embryo, expression begins at day 14 and coincides with the onset of primary myogenesis.1 Publication

Gene expression databases

BgeeiP82350.
ExpressionAtlasiP82350. baseline and differential.
GenevestigatoriP82350.

Interactioni

Subunit structurei

Cross-link to form 2 major subcomplexes: one consisting of SGCB, SGCD and SGCG and the other consisting of SGCB and SGCD. The association between SGCB and SGCG is particularly strong while SGCA is loosely associated with the other sarcoglycans. Interacts with the syntrophin SNTA1.2 Publications

Protein-protein interaction databases

BioGridi203195. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliP82350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sarcoglycan alpha/epsilon family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG323743.
GeneTreeiENSGT00390000005672.
HOGENOMiHOG000074154.
HOVERGENiHBG006891.
InParanoidiP82350.
KOiK12565.
OMAiHTLDHET.
OrthoDBiEOG7MD4PV.
PhylomeDBiP82350.
TreeFamiTF314655.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR028658. Alpha-SG.
IPR006644. Cadg.
IPR015919. Cadherin-like.
IPR013783. Ig-like_fold.
IPR008908. Sarcoglycan_2.
[Graphical view]
PANTHERiPTHR10132. PTHR10132. 1 hit.
PTHR10132:SF1. PTHR10132:SF1. 1 hit.
PfamiPF05510. Sarcoglycan_2. 1 hit.
[Graphical view]
SMARTiSM00736. CADG. 1 hit.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82350-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAVTWIPL LAGLLAGLRD TKAQQTTLHL LVGRVFVHPL EHATFLRLPE
60 70 80 90 100
HVAVPPTVRL TYHAHLQGHP DLPRWLHYTQ RSPYNPGFLY GSPTPEDRGY
110 120 130 140 150
QVIEVTAYNR DSFDTTRQRL LLLIGDPEGP RLPYQAEFLV RSHDVEEVLP
160 170 180 190 200
TTPANRFLTA LGGLWEPGEL QLLNITSALD RGGRVPLPIE GRKEGVYIKV
210 220 230 240 250
GSATPFSTCL KMVASPDSYA RCAQGQPPLL SCYDTLAPHF RVDWCNVSLV
260 270 280 290 300
DKSVPEPLDE VPTPGDGILE HDPFFCPPTE ATDRDFLTDA LVTLLVPLLV
310 320 330 340 350
ALLLTLLLAY IMCFRREGRL KRDMATSDIQ MFHHCSIHGN TEELRQMAAS
360 370 380
REVPRPLSTL PMFNVRTGER LPPRVDSAQM PLILDQH
Length:387
Mass (Da):43,287
Last modified:May 1, 2000 - v1
Checksum:i7C3D98A853D04591
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191R → K in AAB70754. (PubMed:9196068)Curated
Sequence conflicti76 – 761L → V in AAB70754. (PubMed:9196068)Curated
Sequence conflicti149 – 1502LP → VS in AAB70754. (PubMed:9196068)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019564 mRNA. Translation: AAB70754.1.
AB024920 mRNA. Translation: BAA83491.1.
AF064081 Genomic DNA. Translation: AAC33447.1.
AK075915 mRNA. Translation: BAC36051.1.
CCDSiCCDS25266.1.
PIRiJC5556.
RefSeqiNP_033187.1. NM_009161.4.
UniGeneiMm.18709.

Genome annotation databases

EnsembliENSMUST00000100551; ENSMUSP00000098118; ENSMUSG00000001508.
ENSMUST00000103162; ENSMUSP00000099451; ENSMUSG00000001508.
ENSMUST00000166320; ENSMUSP00000130617; ENSMUSG00000001508.
GeneIDi20391.
KEGGimmu:20391.
UCSCiuc007kzo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019564 mRNA. Translation: AAB70754.1.
AB024920 mRNA. Translation: BAA83491.1.
AF064081 Genomic DNA. Translation: AAC33447.1.
AK075915 mRNA. Translation: BAC36051.1.
CCDSiCCDS25266.1.
PIRiJC5556.
RefSeqiNP_033187.1. NM_009161.4.
UniGeneiMm.18709.

3D structure databases

ProteinModelPortaliP82350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203195. 6 interactions.

PTM databases

PhosphoSiteiP82350.

Proteomic databases

PRIDEiP82350.

Protocols and materials databases

DNASUi20391.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100551; ENSMUSP00000098118; ENSMUSG00000001508.
ENSMUST00000103162; ENSMUSP00000099451; ENSMUSG00000001508.
ENSMUST00000166320; ENSMUSP00000130617; ENSMUSG00000001508.
GeneIDi20391.
KEGGimmu:20391.
UCSCiuc007kzo.2. mouse.

Organism-specific databases

CTDi6442.
MGIiMGI:894698. Sgca.

Phylogenomic databases

eggNOGiNOG323743.
GeneTreeiENSGT00390000005672.
HOGENOMiHOG000074154.
HOVERGENiHBG006891.
InParanoidiP82350.
KOiK12565.
OMAiHTLDHET.
OrthoDBiEOG7MD4PV.
PhylomeDBiP82350.
TreeFamiTF314655.

Miscellaneous databases

NextBioi298330.
PROiP82350.
SOURCEiSearch...

Gene expression databases

BgeeiP82350.
ExpressionAtlasiP82350. baseline and differential.
GenevestigatoriP82350.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR028658. Alpha-SG.
IPR006644. Cadg.
IPR015919. Cadherin-like.
IPR013783. Ig-like_fold.
IPR008908. Sarcoglycan_2.
[Graphical view]
PANTHERiPTHR10132. PTHR10132. 1 hit.
PTHR10132:SF1. PTHR10132:SF1. 1 hit.
PfamiPF05510. Sarcoglycan_2. 1 hit.
[Graphical view]
SMARTiSM00736. CADG. 1 hit.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse adhalin: primary structure and expression during late stages of muscle differentiation in vitro."
    Liu L., Vachon P.H., Kuang W., Xu H., Wewer U.M., Kylsten P., Engvall E.
    Biochem. Biophys. Res. Commun. 235:227-235(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: FVB/N.
    Tissue: Myoblast.
  2. "Developmental expression of sarcoglycan gene products in cultured myocytes."
    Noguchi S., Wakabayashi E., Imamura M., Yoshida M., Ozawa E.
    Biochem. Biophys. Res. Commun. 262:88-93(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  3. "Alpha-sarcoglycan-deficient mice as an animal model for autosomal recessive limb-girdle muscular dystrophy."
    Duclos F., Straub V., Moore S., Hrstka R., Crosbie R.H., Durbeej M., Lebakken C.S., Holt K.H., Lim L.E., Ettinger A., Sanes J.R., Davidson B.L., Williamson R., Campbell K.P.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129/Sv.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Tongue.
  5. "Interactions between dystrophin glycoprotein complex proteins."
    Madhavan R., Jarrett H.W.
    Biochemistry 34:12204-12209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNTA1.
  6. "Molecular organization of sarcoglycan complex in mouse myotubes in culture."
    Chan Y.-M., Boennemann C.G., Lidov H.G.W., Kunkel L.M.
    J. Cell Biol. 143:2033-2044(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT.

Entry informationi

Entry nameiSGCA_MOUSE
AccessioniPrimary (citable) accession number: P82350
Secondary accession number(s): O35311, O88490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.