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P82350

- SGCA_MOUSE

UniProt

P82350 - SGCA_MOUSE

Protein

Alpha-sarcoglycan

Gene

Sgca

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    GO - Biological processi

    1. membrane organization Source: MGI
    2. muscle contraction Source: InterPro
    3. muscle organ development Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-sarcoglycan
    Short name:
    Alpha-SG
    Alternative name(s):
    50 kDa dystrophin-associated glycoprotein
    Short name:
    50DAG
    Adhalin
    Gene namesi
    Name:Sgca
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:894698. Sgca.

    Subcellular locationi

    Cell membranesarcolemma 1 Publication; Single-pass type I membrane protein 1 Publication. Cytoplasmcytoskeleton 1 Publication

    GO - Cellular componenti

    1. cell-cell junction Source: MGI
    2. cytoplasm Source: UniProtKB-KW
    3. cytoskeleton Source: UniProtKB-SubCell
    4. dystroglycan complex Source: MGI
    5. integral component of plasma membrane Source: MGI
    6. membrane raft Source: MGI
    7. sarcoglycan complex Source: InterPro
    8. sarcolemma Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 387364Alpha-sarcoglycanPRO_0000031675Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP82350.

    PTM databases

    PhosphoSiteiP82350.

    Expressioni

    Tissue specificityi

    Striated muscle, both skeletal and cardiac.1 Publication

    Developmental stagei

    In the embryo, expression begins at day 14 and coincides with the onset of primary myogenesis.1 Publication

    Gene expression databases

    ArrayExpressiP82350.
    BgeeiP82350.
    GenevestigatoriP82350.

    Interactioni

    Subunit structurei

    Cross-link to form 2 major subcomplexes: one consisting of SGCB, SGCD and SGCG and the other consisting of SGCB and SGCD. The association between SGCB and SGCG is particularly strong while SGCA is loosely associated with the other sarcoglycans. Interacts with the syntrophin SNTA1.2 Publications

    Protein-protein interaction databases

    BioGridi203195. 6 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP82350.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 293270ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini315 – 38773CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei294 – 31421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the sarcoglycan alpha/epsilon family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG323743.
    GeneTreeiENSGT00390000005672.
    HOGENOMiHOG000074154.
    HOVERGENiHBG006891.
    InParanoidiP82350.
    KOiK12565.
    OMAiHTLDHET.
    OrthoDBiEOG7MD4PV.
    PhylomeDBiP82350.
    TreeFamiTF314655.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR028658. Alpha-SG.
    IPR006644. Cadg.
    IPR015919. Cadherin-like.
    IPR013783. Ig-like_fold.
    IPR008908. Sarcoglycan_2.
    [Graphical view]
    PANTHERiPTHR10132. PTHR10132. 1 hit.
    PTHR10132:SF1. PTHR10132:SF1. 1 hit.
    PfamiPF05510. Sarcoglycan_2. 1 hit.
    [Graphical view]
    SMARTiSM00736. CADG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49313. SSF49313. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P82350-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAVTWIPL LAGLLAGLRD TKAQQTTLHL LVGRVFVHPL EHATFLRLPE    50
    HVAVPPTVRL TYHAHLQGHP DLPRWLHYTQ RSPYNPGFLY GSPTPEDRGY 100
    QVIEVTAYNR DSFDTTRQRL LLLIGDPEGP RLPYQAEFLV RSHDVEEVLP 150
    TTPANRFLTA LGGLWEPGEL QLLNITSALD RGGRVPLPIE GRKEGVYIKV 200
    GSATPFSTCL KMVASPDSYA RCAQGQPPLL SCYDTLAPHF RVDWCNVSLV 250
    DKSVPEPLDE VPTPGDGILE HDPFFCPPTE ATDRDFLTDA LVTLLVPLLV 300
    ALLLTLLLAY IMCFRREGRL KRDMATSDIQ MFHHCSIHGN TEELRQMAAS 350
    REVPRPLSTL PMFNVRTGER LPPRVDSAQM PLILDQH 387
    Length:387
    Mass (Da):43,287
    Last modified:May 1, 2000 - v1
    Checksum:i7C3D98A853D04591
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191R → K in AAB70754. (PubMed:9196068)Curated
    Sequence conflicti76 – 761L → V in AAB70754. (PubMed:9196068)Curated
    Sequence conflicti149 – 1502LP → VS in AAB70754. (PubMed:9196068)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019564 mRNA. Translation: AAB70754.1.
    AB024920 mRNA. Translation: BAA83491.1.
    AF064081 Genomic DNA. Translation: AAC33447.1.
    AK075915 mRNA. Translation: BAC36051.1.
    CCDSiCCDS25266.1.
    PIRiJC5556.
    RefSeqiNP_033187.1. NM_009161.4.
    UniGeneiMm.18709.

    Genome annotation databases

    EnsembliENSMUST00000100551; ENSMUSP00000098118; ENSMUSG00000001508.
    ENSMUST00000103162; ENSMUSP00000099451; ENSMUSG00000001508.
    ENSMUST00000166320; ENSMUSP00000130617; ENSMUSG00000001508.
    GeneIDi20391.
    KEGGimmu:20391.
    UCSCiuc007kzo.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019564 mRNA. Translation: AAB70754.1 .
    AB024920 mRNA. Translation: BAA83491.1 .
    AF064081 Genomic DNA. Translation: AAC33447.1 .
    AK075915 mRNA. Translation: BAC36051.1 .
    CCDSi CCDS25266.1.
    PIRi JC5556.
    RefSeqi NP_033187.1. NM_009161.4.
    UniGenei Mm.18709.

    3D structure databases

    ProteinModelPortali P82350.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203195. 6 interactions.

    PTM databases

    PhosphoSitei P82350.

    Proteomic databases

    PRIDEi P82350.

    Protocols and materials databases

    DNASUi 20391.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000100551 ; ENSMUSP00000098118 ; ENSMUSG00000001508 .
    ENSMUST00000103162 ; ENSMUSP00000099451 ; ENSMUSG00000001508 .
    ENSMUST00000166320 ; ENSMUSP00000130617 ; ENSMUSG00000001508 .
    GeneIDi 20391.
    KEGGi mmu:20391.
    UCSCi uc007kzo.2. mouse.

    Organism-specific databases

    CTDi 6442.
    MGIi MGI:894698. Sgca.

    Phylogenomic databases

    eggNOGi NOG323743.
    GeneTreei ENSGT00390000005672.
    HOGENOMi HOG000074154.
    HOVERGENi HBG006891.
    InParanoidi P82350.
    KOi K12565.
    OMAi HTLDHET.
    OrthoDBi EOG7MD4PV.
    PhylomeDBi P82350.
    TreeFami TF314655.

    Miscellaneous databases

    NextBioi 298330.
    PROi P82350.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P82350.
    Bgeei P82350.
    Genevestigatori P82350.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR028658. Alpha-SG.
    IPR006644. Cadg.
    IPR015919. Cadherin-like.
    IPR013783. Ig-like_fold.
    IPR008908. Sarcoglycan_2.
    [Graphical view ]
    PANTHERi PTHR10132. PTHR10132. 1 hit.
    PTHR10132:SF1. PTHR10132:SF1. 1 hit.
    Pfami PF05510. Sarcoglycan_2. 1 hit.
    [Graphical view ]
    SMARTi SM00736. CADG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49313. SSF49313. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mouse adhalin: primary structure and expression during late stages of muscle differentiation in vitro."
      Liu L., Vachon P.H., Kuang W., Xu H., Wewer U.M., Kylsten P., Engvall E.
      Biochem. Biophys. Res. Commun. 235:227-235(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: FVB/N.
      Tissue: Myoblast.
    2. "Developmental expression of sarcoglycan gene products in cultured myocytes."
      Noguchi S., Wakabayashi E., Imamura M., Yoshida M., Ozawa E.
      Biochem. Biophys. Res. Commun. 262:88-93(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    3. "Alpha-sarcoglycan-deficient mice as an animal model for autosomal recessive limb-girdle muscular dystrophy."
      Duclos F., Straub V., Moore S., Hrstka R., Crosbie R.H., Durbeej M., Lebakken C.S., Holt K.H., Lim L.E., Ettinger A., Sanes J.R., Davidson B.L., Williamson R., Campbell K.P.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 129/Sv.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Tongue.
    5. "Interactions between dystrophin glycoprotein complex proteins."
      Madhavan R., Jarrett H.W.
      Biochemistry 34:12204-12209(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNTA1.
    6. "Molecular organization of sarcoglycan complex in mouse myotubes in culture."
      Chan Y.-M., Boennemann C.G., Lidov H.G.W., Kunkel L.M.
      J. Cell Biol. 143:2033-2044(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, SUBUNIT.

    Entry informationi

    Entry nameiSGCA_MOUSE
    AccessioniPrimary (citable) accession number: P82350
    Secondary accession number(s): O35311, O88490
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3