Reviewed, UniProtKB/Swiss-Prot P82320 (DEF6_MACMU)

Names and origin

Protein names	Recommended name:     Neutrophil defensin 6 Alternative name(s):     RMAD-6 Cleaved into the following chain:     1- Recommended name:             Neutrophil defensin 7         Alternative name(s):             RMAD-7
Organism	Macaca mulatta (Rhesus macaque)
Taxonomic identifier	9544 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defensins 6 and 7 have bacteriostatic activity against Gram-positive bacteria S.aureus and L.monocytogenes and Gram-negative bacterium E.coli and antifungal activity against C.neoformans. Defensin 7 has microbicidial activity against Gram-positive bacteria S.aureus and L.monocytogenes.
Subcellular location	Secreted.
Sequence similarities	Belongs to the alpha-defensin family.
Mass spectrometry	Molecular mass is 3898.0 Da from positions 62 - 94. Determined by MALDI. Ref.1 Molecular mass is 3740.0 Da from positions 63 - 94. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Defensin Fungicide
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 61	42	Ref.1		PRO_0000006802
	Peptide	62 – 94	33	Neutrophil defensin 6		PRO_0000006803
	Peptide	63 – 94	32	Neutrophil defensin 7		PRO_0000006804
Amino acid modifications
	Disulfide bond	65 ↔ 92		By similarity
	Disulfide bond	67 ↔ 82		By similarity
	Disulfide bond	72 ↔ 93		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P82320-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E06932080A06CCD2	FASTA	94	10,307
	10 20 30 40 50 60 MRTIAILAAI LLFALLAQAK SLQETADEAA TQEQPGEDDQ DLAVSFEENG LSTLRASGSQ 70 80 90 ARRTCRCRFG RCFRRESYSG SCNINGRISS LCCR

References

[1]

"Isolation, characterization, cDNA cloning, and antimicrobial properties of two distinct subfamilies of alpha-defensins from rhesus macaque leukocytes." Tang Y.Q., Yuan J., Miller C.J., Selsted M.E. Infect. Immun. 67:6139-6144(1999) [PubMed: 10531277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 62-94, MASS SPECTROMETRY. Tissue: Bone marrow and Leukocyte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF188272 mRNA. Translation: AAF06316.1.
PIR	F59076. G59076.
UniGene	Mmu.3478
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMMUG00000017957. Macaca mulatta. [Contig view]
Phylogenomic databases
HOVERGEN	P82320.
Family and domain databases
InterPro	IPR016327. Alpha-defensin. IPR006081. Defensin_alpha. IPR006080. Defensin_mammal. IPR002366. Defensin_propep. [Graphical view]
Pfam	PF00323. Defensin_1. 1 hit. PF00879. Defensin_propep. 1 hit. [Graphical view]
PIRSF	PIRSF001875. Alpha-defensin. 1 hit.
SMART	SM00048. DEFSN. 1 hit. [Graphical view]
PROSITE	PS00269. DEFENSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEF6_MACMU

Accession

Primary (citable) accession number: P82320

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	May 1, 2000
Last modified:	March 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)