Reviewed,
UniProtKB/Swiss-Prot P82281 (APX4_ARATH)
Last modified
November 25, 2008.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Putative L-ascorbate peroxidase 4, chloroplastic EC=1.11.1.11 Alternative name(s): Thylakoid lumenal 29 kDa protein Short name=TL29 P29 AtAPx07 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 349 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Plays a key role in hydrogen peroxide removal By similarity. |
| Catalytic activity | L-ascorbate + H(2)O(2) = dehydroascorbate + 2 H(2)O. |
| Subcellular location | |
| Developmental stage | Down-regulated during leaf senescence. |
| Sequence similarities | Belongs to the peroxidase family. Ascorbate peroxidase subfamily. |
| Caution | Lacks the heme-binding site, the proton acceptor and the transition state stabilizer, which are conserved features of the ascorbate peroxidase. |
Ontologies
Keywords | |
|---|---|
| Biological process | Hydrogen peroxide |
| Cellular component | Chloroplast Plastid Thylakoid |
| Domain | Transit peptide |
| Molecular function | Oxidoreductase Peroxidase |
| Technical term | Complete proteome Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-KW thylakoidInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | L-ascorbate peroxidase activity Inferred from electronic annotation. Source: EC heme bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Chloroplast Potential | |||||||
| Transit peptide | ? – 82 | Thylakoid | |||||||
| Chain | 83 – 349 | 267 | Putative L-ascorbate peroxidase 4, chloroplastic | PRO_0000023635 | |||||
Experimental info | |||||||||
| Sequence conflict | 96 | 1 | S → A in AAP72143. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [3] | "Heat stress- and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis." Panchuk I.I., Volkov R.A., Schoffl F. Plant Physiol. 129:838-853(2002) [PubMed: 12068123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-306. Strain: cv. Columbia. Tissue: Leaf. |
| [4] | "A peroxidase homologue and novel plastocyanin located by proteomics to the Arabidopsis chloroplast thylakoid lumen." Kieselbach T., Bystedt M., Hynds P., Robinson C., Schroeder W.P. FEBS Lett. 480:271-276(2000) [PubMed: 11034343] [Abstract] Cited for: PROTEIN SEQUENCE OF 83-112, SUBCELLULAR LOCATION. Strain: cv. Columbia. |
| [5] | "Expression of the Apx gene family during leaf senescence of Arabidopsis thaliana." Panchuk I.I., Zentgraf U., Volkov R.A. Planta 222:926-932(2005) [PubMed: 16034597] [Abstract] Cited for: DEVELOPMENTAL STAGE. |
Cross-references
Sequence databases | |
|---|---|
| AC005359 Genomic DNA. No translation available. AL161513 Genomic DNA. Translation: CAB78025.1. AF370534 mRNA. Translation: AAK48961.1. AY072503 mRNA. Translation: AAL66918.1. AF441713 mRNA. Translation: AAP72143.1. | |
| PIR | A85091. |
| RefSeq | NP_192640.1. |
| UniGene | At.22637 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1APX based on UniProtKB P48534. |
| ModBase | Search... |
Protein family/group databases | |
| PeroxiBase | 3920. AtAPx07. |
Genome annotation databases | |
| GeneID | 826480. |
| GenomeReviews | Gene locus AT4G09010 in contig CT486007_GR. |
| KEGG | ath:AT4G09010. |
| NMPDR | fig|3702.1.peg.18558. |
Organism-specific databases | |
| GeneFarm | 1959. 146. |
| TAIR | At4g09010. |
Gene expression databases | |
| GermOnline | AT4G09010. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR002207. Asc_perxdse. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view] |
| Pfam | PF00141. peroxidase. 1 hit. [Graphical view] |
| PRINTS | PR00459. ASPEROXIDASE. PR00458. PEROXIDASE. |
| PROSITE | PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | APX4_ARATH | ||||||||
| Accession | Primary (citable) accession number: P82281 Secondary accession number(s): Q7XZP6, Q9M0S6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


