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Protein

Glutamate dehydrogenase, mitochondrial

Gene

glud1

Organism
Chaenocephalus aceratus (Blackfin icefish) (Chaenichthys aceratus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (By similarity).By similarity

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.

Enzyme regulationi

Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP (By similarity).By similarity

pH dependencei

Optimum pH is 8.0.

Temperature dependencei

Optimum temperature is 30 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93SubstrateBy similarity1
Binding sitei117SubstrateBy similarity1
Binding sitei122NADBy similarity1
Active sitei129By similarity1
Binding sitei198NADBy similarity1
Binding sitei212GTPBy similarity1
Binding sitei216GTPBy similarity1
Binding sitei265GTPBy similarity1
Binding sitei268GTPBy similarity1
Binding sitei384SubstrateBy similarity1
Binding sitei390NADBy similarity1
Binding sitei396ADPBy similarity1
Binding sitei462ADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi87 – 89NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

ATP-binding, GTP-binding, NAD, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate dehydrogenase, mitochondrial (EC:1.4.1.3)
Short name:
GDH
Gene namesi
Name:glud1
Synonyms:glud
OrganismiChaenocephalus aceratus (Blackfin icefish) (Chaenichthys aceratus)
Taxonomic identifieri36190 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataEupercariaPerciformesNotothenioideiChannichthyidaeChaenocephalus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001827431 – 504Glutamate dehydrogenase, mitochondrialAdd BLAST504

Proteomic databases

PRIDEiP82264.

Interactioni

Subunit structurei

Homohexamer.

Structurei

3D structure databases

ProteinModelPortaliP82264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG005479.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P82264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ADAADKPDDP NFFRMVEGFF DRGASIVEDK LVEDLRTKET PEQKKGRVAG
60 70 80 90 100
ILRIIKPCNH VLSLSFPIKR DNGEWEVIEG YRAQHSQHRT PCKGGIRYSM
110 120 130 140 150
DVSVDEVKAL ASLMTYKCAV VDVPFGGAKA GVRINTKNYS DNELEKITRR
160 170 180 190 200
FTIELAKKGF IGPGIDVPAP DMSTGEREMS WIADTYANTI AHTDINAHAC
210 220 230 240 250
VTGKPISQGG IHGRISATGR GVFHGIENFM NEASYMSMVG LTPGVQDKTF
260 270 280 290 300
VIQGFGNVGL HSMRYLHRFG AKCVGIGEID GAIYNADGID PKALEEYKLQ
310 320 330 340 350
NGTIVGFPGA KPYEGSILEA DCDILIPAAG EKQLTRNNAR RIKAKIIAEG
360 370 380 390 400
ANGPTTPDAD KIFLENNVMV IPDMYLNAGG VTVSYFEWLK NLNHVSYGRL
410 420 430 440 450
TFKYERDSNY HLLMSVQESL ERKFGKQGGP IPVVPTADFQ ARVAGASEKD
460 470 480 490 500
IVHSGLAYTM ERSARQIMRT ASKHNLGLDI RTAAYVNAIE KVFKVYNEAG

LTFT
Length:504
Mass (Da):55,394
Last modified:March 1, 2001 - v1
Checksum:iD8E20681642E3E89
GO

Cross-referencesi

3D structure databases

ProteinModelPortaliP82264.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP82264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005479.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHE3_CHAAC
AccessioniPrimary (citable) accession number: P82264
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

ADP can occupy the NADH binding site and activate the enzyme.By similarity

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.