P82242 (PLAL1_PLALA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Major pollen allergen Pla l 1 Alternative name(s): Allergen=Pla l 1
Organism	Plantago lanceolata (English plantain)
Taxonomic identifier	39414 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Plantaginaceae › Plantagineae › Plantago

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Post-translational modification	Exists in two variants: glycosylated and non-glycosylated. Carries a complex, major N-linked glycan, with a alpha-1,3-fucose residue in its structure and probably also a beta-1,2-xylose. The average modification of molecular mass due to glycosylation is approximately 969 Da. Ref.3 There are probably three disulfide bonds By similarity.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. The English plantain pollen is an important cause of pollinosis in the temperate regions of North America, Australia and Europe. The glycan moiety does not seem to constitute a relevant allergenic epitope.
Sequence similarities	Belongs to the Ole e I family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Disease	Allergen
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Major pollen allergen Pla l 1		PRO_0000215117
Amino acid modifications
	Glycosylation	107	1	N-linked (GlcNAc...) Probable
	Disulfide bond	17 ↔ 86		By similarity
	Disulfide bond	20 ↔ 131		By similarity
	Disulfide bond	42 ↔ 74		By similarity
Natural variations
	Natural variant	58	1	D → G in Pla l 1.0102 and 1.0103.
	Natural variant	82	1	S → G in Pla l 1.0103.

Sequences

	Sequence		Length	Mass (Da)
	P82242 [UniParc]. Last modified February 12, 2003. Version 2. Checksum: 62F9B279ED3A4BC6	FASTA	131	14,521
	10 20 30 40 50 60 TQTSHPAKFH VEGEVYCNVC HSRNLINELS ERMAGAQVQL DCKDDSKKVI YSIGGETDQD 70 80 90 100 110 120 GVYRLPVVGY HEDCEIKLVK SSRPDCSEIP KLAKGTIQTS KVDLSKNTTI TEKTRHVKPL 130 SFRAKTDAPG C

References

[1]	"Cloning and expression of biologically active Plantago lanceolata pollen allergen Pla l 1 in the yeast Pichia pastoris." Calabozo B., Diaz-Perales A., Salcedo G., Barber D., Polo F. Biochem. J. 372:889-896(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pollen.
[2]	"Purification and characterization of the main allergen of Plantago lanceolata pollen, Pla l 1." Calabozo B., Barber D., Polo F. Clin. Exp. Allergy 31:322-330(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-18. Tissue: Pollen.
[3]	"Studies on the carbohydrate moiety of Pla l 1 allergen. Identification of a major N-glycan and significance for the immunoglobulin E-binding activity." Calabozo B., Barber D., Polo F. Clin. Exp. Allergy 32:1628-1634(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE OF CARBOHYDRATE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ313166 mRNA. Translation: CAC41633.1. AJ313167 mRNA. Translation: CAC41634.1. AJ313168 mRNA. Translation: CAC41635.1.
3D structure databases
ModBase	Search...
Protein family/group databases
Allergome	1323. Pla l 1.0101. 1324. Pla l 1.0102. 1325. Pla l 1.0103. 574. Pla l 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006041. Pollen_Ole_e1_allergen. [Graphical view]
Pfam	PF01190. Pollen_Ole_e_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLAL1_PLALA

Accession

Primary (citable) accession number: P82242
Secondary accession number(s): Q93X26, Q949A3, Q949A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	February 12, 2003
Last modified:	April 3, 2013
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program