Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cycloviolacin-O1

Gene
N/A
Organism
Viola odorata (Sweet violet)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably participates in a plant defense mechanism.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloviolacin-O1
OrganismiViola odorata (Sweet violet)
Taxonomic identifieri97441 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesViolaceaeViola

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3030Cycloviolacin-O1PRO_0000043609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1 ↔ 30Cyclopeptide (Gly-Asn)
Disulfide bondi4 ↔ 21
Disulfide bondi8 ↔ 23
Disulfide bondi13 ↔ 28

Post-translational modificationi

This is a cyclic peptide.

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
30
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Helixi17 – 193Combined sources
Beta strandi22 – 243Combined sources
Beta strandi27 – 304Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DF6NMR-A7-30[»]
1NBJNMR-A4-30[»]
ProteinModelPortaliP82230.
SMRiP82230. Positions 4-29.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82230.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the cyclotide family. Bracelet subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012323. Cyclotide_bracelet_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
PIRSFiPIRSF037891. Cycloviolacin. 1 hit.
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60008. CYCLOTIDE_BRACELET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P82230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30
GIPCAESCVY IPCTVTALLG CSCSNRVCYN
Length:30
Mass (Da):3,141
Last modified:December 5, 2001 - v2
Checksum:iB6F6DAD5755B8937
GO

Mass spectrometryi

Molecular mass is 3114.4 Da from positions 1 - 30. Determined by MALDI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DF6NMR-A7-30[»]
1NBJNMR-A4-30[»]
ProteinModelPortaliP82230.
SMRiP82230. Positions 4-29.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP82230.

Family and domain databases

InterProiIPR005535. Cyclotide.
IPR012323. Cyclotide_bracelet_CS.
[Graphical view]
PfamiPF03784. Cyclotide. 1 hit.
[Graphical view]
PIRSFiPIRSF037891. Cycloviolacin. 1 hit.
SUPFAMiSSF57038. SSF57038. 1 hit.
PROSITEiPS51052. CYCLOTIDE. 1 hit.
PS60008. CYCLOTIDE_BRACELET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Plant cyclotides: a unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif."
    Craik D.J., Daly N.L., Bond T., Waine C.
    J. Mol. Biol. 294:1327-1336(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, STRUCTURE BY NMR.
  2. "A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability."
    Ireland D.C., Colgrave M.L., Craik D.J.
    Biochem. J. 400:1-12(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY.
  3. "Twists, knots, and rings in proteins. Structural definition of the cyclotide framework."
    Rosengren K.J., Daly N.L., Plan M.R.R., Waine C., Craik D.J.
    J. Biol. Chem. 278:8606-8616(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiCYO1_VIOOD
AccessioniPrimary (citable) accession number: P82230
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 5, 2001
Last modified: December 9, 2015
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

This peptide is cyclic. The start position was chosen by similarity to OAK1 (kalata-B1) for which the DNA sequence is known.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.