Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kappa-hexatoxin-Hv1c

Gene
N/A
Organism
Hadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei2 – 21Important for the neurotoxic activity
Sitei8 – 81Critical for the neurotoxic activity
Sitei9 – 91Critical for the neurotoxic activity
Sitei13 – 131Critical for the neurotoxic activity
Sitei14 – 141Critical for the neurotoxic activity
Sitei29 – 291Important for the neurotoxic activity
Sitei31 – 311Critical for the neurotoxic activity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-hexatoxin-Hv1c
Short name:
Kappa-HXTX-Hv1c
Alternative name(s):
Janus-atracotoxin-Hv1c
Short name:
Janus-AcTx-Hv1c
Kappa-atracotoxin-Hv1c
Short name:
Kappa-AcTx-Hv1c
OrganismiHadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus)
Taxonomic identifieri6904 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeHexathelidaeHadronyche

Organism-specific databases

ArachnoServeriAS000174. kappa-hexatoxin-Hv1c.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 of recombinant toxin is 320 +-20 pmol/g (according to PubMed:11937509) or 91 +-5 pmol/g (according to PubMed:12220722) when injected into house flies (Musca domestica).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 22Missing : 73-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi1 – 11Missing : No change in toxicity. 1 Publication
Mutagenesisi2 – 21I → A: 7-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi8 – 81R → A: 98-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi8 – 81R → E: 270-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi9 – 91P → A: 269-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi13 – 142CC → SS: 423-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi29 – 291V → A: 13-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi31 – 311Y → A: 162-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi31 – 311Y → F: 1.8-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi35 – 373Missing : No change in toxicity to flies.
Mutagenesisi36 – 372Missing : No change in toxicity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3737Kappa-hexatoxin-Hv1cPRO_0000044998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 17
Disulfide bondi10 ↔ 22
Disulfide bondi13 ↔ 14
Disulfide bondi16 ↔ 32

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
37
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 245Combined sources
Beta strandi30 – 345Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DL0NMR-A1-37[»]
ProteinModelPortaliP82228.
SMRiP82228. Positions 1-37.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82228.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the Shiva superfamily. Kappa toxin family.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR012499. Toxin_16.
[Graphical view]
PfamiPF07945. Toxin_16. 1 hit.
[Graphical view]
PROSITEiPS60020. J_ACTX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P82228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
AICTGADRPC AACCPCCPGT SCKAESNGVS YCRKDEP
Length:37
Mass (Da):3,768
Last modified:May 30, 2000 - v1
Checksum:iE4DDF046CC750FFC
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DL0NMR-A1-37[»]
ProteinModelPortaliP82228.
SMRiP82228. Positions 1-37.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000174. kappa-hexatoxin-Hv1c.

Miscellaneous databases

EvolutionaryTraceiP82228.

Family and domain databases

InterProiIPR012499. Toxin_16.
[Graphical view]
PfamiPF07945. Toxin_16. 1 hit.
[Graphical view]
PROSITEiPS60020. J_ACTX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTK1C_HADVE
AccessioniPrimary (citable) accession number: P82228
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: December 9, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Does not affect a wide range of potassium, calcium and sodium voltage-gated channels.1 Publication

Caution

This toxin should be named lambda-hexatoxin-Hv1c (instead of kappa), since lambda is the Greek letter attributed to calcium-activated potassium (KCa) channel impairing toxins (according to the nomenclature of King et al., 2008).Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.