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P82228

- TK1C_HADVE

UniProt

P82228 - TK1C_HADVE

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Protein

Kappa-hexatoxin-Hv1c

Gene
N/A
Organism
Hadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei2 – 21Important for the neurotoxic activity
Sitei8 – 81Critical for the neurotoxic activity
Sitei9 – 91Critical for the neurotoxic activity
Sitei13 – 131Critical for the neurotoxic activity
Sitei14 – 141Critical for the neurotoxic activity
Sitei29 – 291Important for the neurotoxic activity
Sitei31 – 311Critical for the neurotoxic activity

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Calcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-hexatoxin-Hv1c
Short name:
Kappa-HXTX-Hv1c
Alternative name(s):
Janus-atracotoxin-Hv1c
Short name:
Janus-AcTx-Hv1c
Kappa-atracotoxin-Hv1c
Short name:
Kappa-AcTx-Hv1c
OrganismiHadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus)
Taxonomic identifieri6904 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeHexathelidaeHadronyche

Organism-specific databases

ArachnoServeriAS000174. kappa-hexatoxin-Hv1c.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 of recombinant toxin is 320 +-20 pmol/g (according to PubMed:11937509) or 91 +-5 pmol/g (according to PubMed:12220722) when injected into house flies (Musca domestica).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 22Missing: 73-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi1 – 11Missing: No change in toxicity. 1 Publication
Mutagenesisi2 – 21I → A: 7-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi8 – 81R → A: 98-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi8 – 81R → E: 270-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi9 – 91P → A: 269-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi13 – 142CC → SS: 423-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi29 – 291V → A: 13-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi31 – 311Y → A: 162-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi31 – 311Y → F: 1.8-fold decrease in toxicity to flies. 1 Publication
Mutagenesisi35 – 373Missing: No change in toxicity to flies.
Mutagenesisi36 – 372Missing: No change in toxicity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3737Kappa-hexatoxin-Hv1cPRO_0000044998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 17
Disulfide bondi10 ↔ 22
Disulfide bondi13 ↔ 14
Disulfide bondi16 ↔ 32

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
37
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 245
Beta strandi30 – 345

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DL0NMR-A1-37[»]
ProteinModelPortaliP82228.
SMRiP82228. Positions 1-37.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82228.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the Shiva superfamily. Kappa toxin family.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR012499. Toxin_16.
[Graphical view]
PfamiPF07945. Toxin_16. 1 hit.
[Graphical view]
PROSITEiPS60020. J_ACTX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P82228-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30
AICTGADRPC AACCPCCPGT SCKAESNGVS YCRKDEP
Length:37
Mass (Da):3,768
Last modified:May 30, 2000 - v1
Checksum:iE4DDF046CC750FFC
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DL0 NMR - A 1-37 [» ]
ProteinModelPortali P82228.
SMRi P82228. Positions 1-37.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ArachnoServeri AS000174. kappa-hexatoxin-Hv1c.

Miscellaneous databases

EvolutionaryTracei P82228.

Family and domain databases

InterProi IPR012499. Toxin_16.
[Graphical view ]
Pfami PF07945. Toxin_16. 1 hit.
[Graphical view ]
PROSITEi PS60020. J_ACTX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge."
    Wang X.-H., Connor M., Smith R., Maciejewski M.W., Howden M.E.H., Nicholson G.M., Christie M.J., King G.F.
    Nat. Struct. Biol. 7:505-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, STRUCTURE BY NMR.
    Tissue: Venom.
  2. "Scanning mutagenesis of a Janus-faced atracotoxin reveals a bipartite surface patch that is essential for neurotoxic function."
    Maggio F., King G.F.
    J. Biol. Chem. 277:22806-22813(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ILE-2; ARG-8; PRO-9; 13-CYS-CYS-14; VAL-29 AND TYR-31, LETHAL DOSE.
  3. "Role of the structurally disordered N- and C-terminal residues in the Janus-faced atracotoxins."
    Maggio F., King G.F.
    Toxicon 40:1355-1361(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ALA-1; 1-ALA-ILE-2 AND 36-GLU-PRO-37, LETHAL DOSE.
  4. "A model genetic system for testing the in vivo function of peptide toxins."
    Tedford H.W., Maggio F., Reenan R.A., King G.
    Peptides 28:51-56(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Phase coupling of a circadian neuropeptide with rest/activity rhythms detected using a membrane-tethered spider toxin."
    Wu Y., Cao G., Pavlicek B., Luo X., Nitabach M.N.
    PLoS Biol. 6:E273-E273(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The Janus-faced atracotoxins are specific blockers of invertebrate K(Ca) channels."
    Gunning S.J., Maggio F., Windley M.J., Valenzuela S.M., King G.F., Nicholson G.M.
    FEBS J. 275:4045-4059(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTK1C_HADVE
AccessioniPrimary (citable) accession number: P82228
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Does not affect a wide range of potassium, calcium and sodium voltage-gated channels.1 Publication

Caution

This toxin should be named lambda-hexatoxin-Hv1c (instead of kappa), since lambda is the Greek letter attributed to calcium-activated potassium (KCa) channel impairing toxins (according to the nomenclature of King et al., 2008).Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3