P82226 (TJT1B_HADVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Kappa-hexatoxin-Hv1b Short name=Kappa-HXTX-Hv1b Alternative name(s): Janus-atracotoxin-Hv1b Short name=Janus-AcTx-Hv1b Kappa-atracotoxin-Hv1b Short name=Kappa-AcTx-Hv1b
Organism	Hadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus)
Taxonomic identifier	6904 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Araneae › Mygalomorphae › Hexathelidae › Hadronyche

Protein attributes

Sequence length	36 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This excitatory toxin inhibits insect voltage-gated potassium channels. Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Domain	The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.
Sequence similarities	Belongs to the janus-atracotoxin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Knottin
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 36	36	Kappa-hexatoxin-Hv1b		PRO_0000044997
Sites
	Site	2	1	Important for the neurotoxic activity By similarity
	Site	8	1	Critical for the neurotoxic activity By similarity
	Site	9	1	Critical for the neurotoxic activity By similarity
	Site	13	1	Critical for the neurotoxic activity By similarity
	Site	14	1	Critical for the neurotoxic activity By similarity
	Site	30	1	Important for the neurotoxic activity By similarity
	Site	32	1	Critical for the neurotoxic activity By similarity
Amino acid modifications
	Disulfide bond	3 ↔ 17		By similarity
	Disulfide bond	10 ↔ 22		By similarity
	Disulfide bond	13 ↔ 14		By similarity
	Disulfide bond	16 ↔ 33		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P82226 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: D23A442560B89997	FASTA	36	3,651
	10 20 30 TICTGADRPC AACCPCCPGT SCQGPEPNGV SYCRND

References

[1]	"Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge." Wang X.-H., Connor M., Smith R., Maciejewski M.W., Howden M.E.H., Nicholson G.M., Christie M.J., King G.F. Nat. Struct. Biol. 7:505-513(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"Structure and function of delta-atracotoxins: lethal neurotoxins targeting the voltage-gated sodium channel." Nicholson G.M., Little M.J., Birinyi-Strachan L.C. Toxicon 43:587-599(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

3D structure databases
ProteinModelPortal	P82226.
SMR	P82226. Positions 1-36.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ArachnoServer	AS000173. kappa-hexatoxin-Hv1b.
Family and domain databases
InterPro	IPR012499. Toxin_16. [Graphical view]
Pfam	PF07945. Toxin_16. 1 hit. [Graphical view]
PROSITE	PS60020. J_ACTX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TJT1B_HADVE

Accession

Primary (citable) accession number: P82226

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program