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Protein

Pyridoxal kinase

Gene

PDXK

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for synthesis of pyridoxal-5-phosphate from vitamin B6.

Catalytic activityi

ATP + pyridoxal = ADP + pyridoxal 5'-phosphate.

Cofactori

Zn2+, Mg2+Note: Divalent metal cations. Zn2+ is more efficient than Mg2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12Substrate1 Publication1
Binding sitei47Substrate1 Publication1
Binding sitei127Substrate1 Publication1
Binding sitei235Substrate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi186 – 187ATP1 Publication2
Nucleotide bindingi223 – 234ATP1 PublicationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.35. 2668.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal kinase (EC:2.7.1.35)
Alternative name(s):
Pyridoxine kinase
Gene namesi
Name:PDXK
Synonyms:PKH
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002133391 – 312Pyridoxal kinaseAdd BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei59PhosphoserineBy similarity1
Modified residuei164PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei285PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Homodimer.2 Publications

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 17Combined sources12
Helixi21 – 30Combined sources10
Beta strandi34 – 45Combined sources12
Beta strandi54 – 56Combined sources3
Helixi59 – 71Combined sources13
Beta strandi78 – 82Combined sources5
Helixi88 – 104Combined sources17
Beta strandi109 – 112Combined sources4
Beta strandi116 – 118Combined sources3
Beta strandi122 – 124Combined sources3
Beta strandi125 – 128Combined sources4
Helixi130 – 132Combined sources3
Helixi133 – 138Combined sources6
Helixi141 – 143Combined sources3
Beta strandi145 – 147Combined sources3
Helixi151 – 158Combined sources8
Helixi165 – 178Combined sources14
Beta strandi181 – 185Combined sources5
Beta strandi198 – 207Combined sources10
Beta strandi210 – 212Combined sources3
Beta strandi215 – 224Combined sources10
Helixi233 – 247Combined sources15
Helixi252 – 277Combined sources26
Beta strandi280 – 282Combined sources3
Turni286 – 289Combined sources4
Helixi294 – 296Combined sources3
Helixi297 – 301Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LHPX-ray2.10A/B1-312[»]
1LHRX-ray2.60A/B1-312[»]
1RFTX-ray2.80A1-312[»]
1RFUX-ray2.80A/B/C/D/E/F/G/H1-312[»]
1RFVX-ray2.80A/B1-312[»]
1YGJX-ray2.70A1-312[»]
1YGKX-ray2.60A1-312[»]
1YHJX-ray2.80A1-312[»]
ProteinModelPortaliP82197.
SMRiP82197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82197.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyridoxine kinase family.Curated

Phylogenomic databases

HOVERGENiHBG000732.
KOiK00868.

Family and domain databases

CDDicd01173. pyridoxal_pyridoxamine_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
InterProiIPR013749. PM/HMP-P_kinase-1.
IPR004625. PyrdxlKinase.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10534. PTHR10534. 1 hit.
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00687. pyridox_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

P82197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEECRVLSI QSHVVRGYVG NRAATFPLQV LGFEVDAVNS VQFSNHTGYS
60 70 80 90 100
HWKGQVLNSD ELQELYDGLK LNHVNQYDYV LTGYTRDKSF LAMVVDIVQE
110 120 130 140 150
LKQQNPRLVY VCDPVMGDQR NGEGAMYVPD DLLPVYREKV VPVADIITPN
160 170 180 190 200
QFEAELLTGR KIHSQEEALE VMDMLHSMGP DTVVITSSNL LSPRGSDYLM
210 220 230 240 250
ALGSQRTRAP DGSVVTQRIR MEMHKVDAVF VGTGDLFAAM LLAWTHKHPN
260 270 280 290 300
NLKVACEKTV SAMHHVLQRT IKCAKAKSGE GVKPSPAQLE LRMVQSKKDI
310
ESPEIVVQAT VL
Length:312
Mass (Da):34,819
Last modified:May 30, 2000 - v1
Checksum:iC5F286DBCF51CA6A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16 – 47RGYVG…FSNHT → MCFGGTAESLCPGDLMQHRG LTWSALPPTPPP (Ref. 2) CuratedAdd BLAST32
Sequence conflicti61 – 62EL → DV in AAD34353 (Ref. 2) Curated2
Sequence conflicti189N → D in AAD34353 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125374 mRNA. Translation: AAD34353.1.
RefSeqiNP_001009220.1. NM_001009220.1.
UniGeneiOar.696.

Genome annotation databases

GeneIDi443049.
KEGGioas:443049.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125374 mRNA. Translation: AAD34353.1.
RefSeqiNP_001009220.1. NM_001009220.1.
UniGeneiOar.696.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LHPX-ray2.10A/B1-312[»]
1LHRX-ray2.60A/B1-312[»]
1RFTX-ray2.80A1-312[»]
1RFUX-ray2.80A/B/C/D/E/F/G/H1-312[»]
1RFVX-ray2.80A/B1-312[»]
1YGJX-ray2.70A1-312[»]
1YGKX-ray2.60A1-312[»]
1YHJX-ray2.80A1-312[»]
ProteinModelPortaliP82197.
SMRiP82197.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443049.
KEGGioas:443049.

Organism-specific databases

CTDi8566.

Phylogenomic databases

HOVERGENiHBG000732.
KOiK00868.

Enzyme and pathway databases

BRENDAi2.7.1.35. 2668.

Miscellaneous databases

EvolutionaryTraceiP82197.

Family and domain databases

CDDicd01173. pyridoxal_pyridoxamine_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
InterProiIPR013749. PM/HMP-P_kinase-1.
IPR004625. PyrdxlKinase.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10534. PTHR10534. 1 hit.
PfamiPF08543. Phos_pyr_kin. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00687. pyridox_kin. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPDXK_SHEEP
AccessioniPrimary (citable) accession number: P82197
Secondary accession number(s): Q9XSD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.