P82186 (GUN_MYTED) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoglucanase EC=3.2.1.4 Alternative name(s): CMCase Cellulase Endo-1,4-beta-glucanase
Organism	Mytilus edulis (Blue mussel)
Taxonomic identifier	6550 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Bivalvia › Pteriomorphia › Mytiloida › Mytiloidea › Mytilidae › Mytilinae › Mytilus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Active towards the soluble carboxymethylcellulose (CMC). Possesses expansin activity too. Ref.1
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Tissue specificity	Digestive gland.
Sequence similarities	Belongs to the glycosyl hydrolase 45 (cellulase K) family.
Biophysicochemical properties	pH dependence: Optimum pH is 4.6. Temperature dependence: Optimum temperature is 30-50 degrees Celsius.
Mass spectrometry	Molecular mass is 19702 Da from positions 1 - 181. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 181

181

Endoglucanase

PRO_0000184075

Sites

Active site

24

1

Nucleophile By similarity

Active site

132

1

Proton donor By similarity

Amino acid modifications

Disulfide bond

4 ↔ 16

Disulfide bond

30 ↔ 69

Disulfide bond

32 ↔ 176

Disulfide bond

65 ↔ 178

Disulfide bond

72 ↔ 157

Disulfide bond

103 ↔ 113

Secondary structure

1

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181

Helix Strand Turn

Details...

Turn

6 – 9

4

Beta strand

14 – 22

9

Turn

28 – 31

4

Beta strand

35 – 39

5

Helix

44 – 46

3

Beta strand

49 – 53

5

Helix

54 – 57

4

Beta strand

63 – 65

3

Turn

67 – 70

4

Beta strand

72 – 81

10

Beta strand

94 – 103

10

Turn

110 – 112

3

Beta strand

116 – 120

5

Beta strand

128 – 134

7

Helix

141 – 143

3

Beta strand

146 – 155

10

Helix

157 – 163

7

Helix

170 – 173

4

Helix

177 – 179

3

Sequences

	Sequence		Length	Mass (Da)
	P82186 [UniParc]. Last modified January 11, 2001. Version 1. Checksum: E00A8C57203823F6	FASTA	181	19,711
	10 20 30 40 50 60 NQKCSGNPRR YNGKSCASTT NYHDSHKGAC GCGPASGDAQ FGWNAGSFVA AASQMYFDSG 70 80 90 100 110 120 NKGWCGQHCG QCIKLTTTGG YVPGQGGPVR EGLSKTFMIT NLCPNIYPNQ DWCNQGSQYG 130 140 150 160 170 180 GHNKYGYELH LDLENGRSQV TGMGWNNPET TWEVVNCDSE HNHDHRTPSN SMYGQCQCAH Q

References

[1]

"Purification, characterization and amino-acid sequence analysis of a thermostable, low molecular mass endo-beta-1,4-glucanase from blue mussel, Mytilus edulis." Xu B., Hellman U., Ersson B., Janson J.-C. Eur. J. Biochem. 267:4970-4977(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY. Tissue: Digestive gland.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WC2	X-ray	1.20	A	1-181	[»]

ProteinModelPortal

P82186.

SMR

P82186. Positions 1-180.

ModBase

Search...

Protein family/group databases

CAZy

GH45. Glycoside Hydrolase Family 45.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009009. Barwin-related_endoglucanase.
[Graphical view]

SUPFAM

SSF50685. Barwin_like. 1 hit.

PROSITE

PS01140. GLYCOSYL_HYDROL_F45. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P82186.

Entry information

Entry name

GUN_MYTED

Accession

Primary (citable) accession number: P82186

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)