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P82186

- GUN_MYTED

UniProt

P82186 - GUN_MYTED

Protein

Endoglucanase

Gene
N/A
Organism
Mytilus edulis (Blue mussel)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Active towards the soluble carboxymethylcellulose (CMC). Possesses expansin activity too.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    pH dependencei

    Optimum pH is 4.6.

    Temperature dependencei

    Optimum temperature is 30-50 degrees Celsius.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei24 – 241NucleophileBy similarity
    Active sitei132 – 1321Proton donorBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH45. Glycoside Hydrolase Family 45.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase (EC:3.2.1.4)
    Alternative name(s):
    CMCase
    Cellulase
    Endo-1,4-beta-glucanase
    OrganismiMytilus edulis (Blue mussel)
    Taxonomic identifieri6550 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaPteriomorphiaMytiloidaMytiloideaMytilidaeMytilinaeMytilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181EndoglucanasePRO_0000184075Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi4 ↔ 16
    Disulfide bondi30 ↔ 69
    Disulfide bondi32 ↔ 176
    Disulfide bondi65 ↔ 178
    Disulfide bondi72 ↔ 157
    Disulfide bondi103 ↔ 113

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Digestive gland.

    Structurei

    Secondary structure

    1
    181
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni6 – 94
    Beta strandi14 – 229
    Turni28 – 314
    Beta strandi35 – 395
    Helixi44 – 463
    Beta strandi49 – 535
    Helixi54 – 574
    Beta strandi63 – 653
    Turni67 – 704
    Beta strandi72 – 8110
    Beta strandi94 – 10310
    Turni110 – 1123
    Beta strandi116 – 1205
    Beta strandi128 – 1347
    Helixi141 – 1433
    Beta strandi146 – 15510
    Helixi157 – 1637
    Helixi170 – 1734
    Helixi177 – 1793

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WC2X-ray1.20A1-181[»]
    ProteinModelPortaliP82186.
    SMRiP82186. Positions 1-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP82186.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR009009. RlpA-like_DPBB.
    [Graphical view]
    SUPFAMiSSF50685. SSF50685. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P82186-1 [UniParc]FASTAAdd to Basket

    « Hide

    NQKCSGNPRR YNGKSCASTT NYHDSHKGAC GCGPASGDAQ FGWNAGSFVA    50
    AASQMYFDSG NKGWCGQHCG QCIKLTTTGG YVPGQGGPVR EGLSKTFMIT 100
    NLCPNIYPNQ DWCNQGSQYG GHNKYGYELH LDLENGRSQV TGMGWNNPET 150
    TWEVVNCDSE HNHDHRTPSN SMYGQCQCAH Q 181
    Length:181
    Mass (Da):19,711
    Last modified:January 11, 2001 - v1
    Checksum:iE00A8C57203823F6
    GO

    Mass spectrometryi

    Molecular mass is 19702 Da from positions 1 - 181. Determined by MALDI. 1 Publication

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WC2 X-ray 1.20 A 1-181 [» ]
    ProteinModelPortali P82186.
    SMRi P82186. Positions 1-180.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH45. Glycoside Hydrolase Family 45.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P82186.

    Family and domain databases

    InterProi IPR009009. RlpA-like_DPBB.
    [Graphical view ]
    SUPFAMi SSF50685. SSF50685. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification, characterization and amino-acid sequence analysis of a thermostable, low molecular mass endo-beta-1,4-glucanase from blue mussel, Mytilus edulis."
      Xu B., Hellman U., Ersson B., Janson J.-C.
      Eur. J. Biochem. 267:4970-4977(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY.
      Tissue: Digestive gland.

    Entry informationi

    Entry nameiGUN_MYTED
    AccessioniPrimary (citable) accession number: P82186
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3