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Protein

Malate dehydrogenase

Gene

mdh

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADBy similarity1
Binding sitei81SubstrateBy similarity1
Binding sitei87SubstrateBy similarity1
Binding sitei94NADBy similarity1
Binding sitei119SubstrateBy similarity1
Binding sitei153SubstrateBy similarity1
Active sitei177Proton acceptorBy similarity1
Binding sitei227NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 13NADBy similarity7
Nucleotide bindingi117 – 119NADBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: UniProtKB-EC
  • malate dehydrogenase activity Source: TIGR
  • malic enzyme activity Source: TIGR

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • malate metabolic process Source: InterPro
  • tricarboxylic acid cycle Source: TIGR

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciSONE211586:G1GMP-721-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:SO_0770
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000008186 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001133261 – 311Malate dehydrogenaseAdd BLAST311

Proteomic databases

PaxDbiP82177
PRIDEiP82177

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi211586.SO_0770

Structurei

3D structure databases

ProteinModelPortaliP82177
SMRiP82177
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213792
KOiK00024
OMAiQCTPKVE
OrthoDBiPOG091H036H
PhylomeDBiP82177

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01516 Malate_dehydrog_1, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR023958 Malate_DH_type1_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR11540:SF22 PTHR11540:SF22, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

P82177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVLGAAG GIGQALALLL KTQLPAGSKL SLYDIAPVTP GVAVDLSHIP
60 70 80 90 100
TAVEIKGFAG EDPTPALVGA DVVLISAGVA RKPGMDRSDL FNINAGIVRN
110 120 130 140 150
LIEKVAVTCP KALVGIITNP VNTTVAIAAE VMKKAGVYDK NRLFGVTTLD
160 170 180 190 200
VIRSETFIAE LKGLNVADVK INVIGGHSGV TILPLLSQVE GVTFSDEEVA
210 220 230 240 250
SLTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGMSLVR GLQGEANVVE
260 270 280 290 300
CAYVDGGSEH AEFFAQPVLL GKNGIEKVLP YGEVSAFEAN ARDSMLDTLK
310
GDIKLGVDFV K
Length:311
Mass (Da):32,137
Last modified:November 15, 2002 - v2
Checksum:i9D3F5E86A2500A1B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13Missing AA sequence (PubMed:11135424).Curated1
Sequence conflicti16L → LG AA sequence (PubMed:11135424).Curated1

Mass spectrometryi

Molecular mass is 1734.8 Da from positions 3 - 21. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014299 Genomic DNA Translation: AAN53846.1
RefSeqiNP_716401.1, NC_004347.2
WP_011071073.1, NC_004347.2

Genome annotation databases

EnsemblBacteriaiAAN53846; AAN53846; SO_0770
GeneIDi1168625
KEGGison:SO_0770
PATRICifig|211586.12.peg.740

Similar proteinsi

Entry informationi

Entry nameiMDH_SHEON
AccessioniPrimary (citable) accession number: P82177
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 15, 2002
Last modified: March 28, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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