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P82177

- MDH_SHEON

UniProt

P82177 - MDH_SHEON

Protein

Malate dehydrogenase

Gene

mdh

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (15 Nov 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADBy similarity
    Binding sitei81 – 811SubstrateBy similarity
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei94 – 941NADBy similarity
    Binding sitei119 – 1191SubstrateBy similarity
    Binding sitei153 – 1531SubstrateBy similarity
    Active sitei177 – 1771Proton acceptorBy similarity
    Binding sitei227 – 2271NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 137NADBy similarity
    Nucleotide bindingi117 – 1193NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    Ordered Locus Names:SO_0770
    OrganismiShewanella oneidensis (strain MR-1)
    Taxonomic identifieri211586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000008186: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 311311Malate dehydrogenasePRO_0000113326Add
    BLAST

    Proteomic databases

    PRIDEiP82177.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi211586.SO_0770.

    Structurei

    3D structure databases

    ProteinModelPortaliP82177.
    SMRiP82177. Positions 1-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213792.
    KOiK00024.
    OMAiVEVKGFA.
    OrthoDBiEOG6091FG.
    PhylomeDBiP82177.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01516. Malate_dehydrog_1.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR023958. Malate_DH_type1_bac.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P82177-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVAVLGAAG GIGQALALLL KTQLPAGSKL SLYDIAPVTP GVAVDLSHIP    50
    TAVEIKGFAG EDPTPALVGA DVVLISAGVA RKPGMDRSDL FNINAGIVRN 100
    LIEKVAVTCP KALVGIITNP VNTTVAIAAE VMKKAGVYDK NRLFGVTTLD 150
    VIRSETFIAE LKGLNVADVK INVIGGHSGV TILPLLSQVE GVTFSDEEVA 200
    SLTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGMSLVR GLQGEANVVE 250
    CAYVDGGSEH AEFFAQPVLL GKNGIEKVLP YGEVSAFEAN ARDSMLDTLK 300
    GDIKLGVDFV K 311
    Length:311
    Mass (Da):32,137
    Last modified:November 15, 2002 - v2
    Checksum:i9D3F5E86A2500A1B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131Missing AA sequence (PubMed:11135424)Curated
    Sequence conflicti16 – 161L → LG AA sequence (PubMed:11135424)Curated

    Mass spectrometryi

    Molecular mass is 1734.8 Da from positions 3 - 21. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014299 Genomic DNA. Translation: AAN53846.1.
    RefSeqiNP_716401.1. NC_004347.2.
    WP_011071073.1. NC_004347.2.

    Genome annotation databases

    EnsemblBacteriaiAAN53846; AAN53846; SO_0770.
    GeneIDi1168625.
    KEGGison:SO_0770.
    PATRICi23521213. VBISheOne101494_0740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014299 Genomic DNA. Translation: AAN53846.1 .
    RefSeqi NP_716401.1. NC_004347.2.
    WP_011071073.1. NC_004347.2.

    3D structure databases

    ProteinModelPortali P82177.
    SMRi P82177. Positions 1-310.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 211586.SO_0770.

    Proteomic databases

    PRIDEi P82177.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN53846 ; AAN53846 ; SO_0770 .
    GeneIDi 1168625.
    KEGGi son:SO_0770.
    PATRICi 23521213. VBISheOne101494_0740.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213792.
    KOi K00024.
    OMAi VEVKGFA.
    OrthoDBi EOG6091FG.
    PhylomeDBi P82177.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01516. Malate_dehydrog_1.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR023958. Malate_DH_type1_bac.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MR-1.
    2. "Automated nanoflow liquid chromatography/tandem mass spectrometric identification of proteins from Shewanella putrefaciens separated by two-dimensional polyacrylamide gel electrophoresis."
      Devreese B., Vanrobaeys F., Van Beeumen J.
      Rapid Commun. Mass Spectrom. 15:50-56(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 3-21, MASS SPECTROMETRY.
      Strain: MR-1.

    Entry informationi

    Entry nameiMDH_SHEON
    AccessioniPrimary (citable) accession number: P82177
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 15, 2002
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3