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P82177

- MDH_SHEON

UniProt

P82177 - MDH_SHEON

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Protein
Malate dehydrogenase
Gene
mdh, SO_0770
Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NAD By similarity
Binding sitei81 – 811Substrate By similarity
Binding sitei87 – 871Substrate By similarity
Binding sitei94 – 941NAD By similarity
Binding sitei119 – 1191Substrate By similarity
Binding sitei153 – 1531Substrate By similarity
Active sitei177 – 1771Proton acceptor By similarity
Binding sitei227 – 2271NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 137NAD By similarity
Nucleotide bindingi117 – 1193NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:SO_0770
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000008186: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Malate dehydrogenaseUniRule annotation
PRO_0000113326Add
BLAST

Proteomic databases

PRIDEiP82177.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi211586.SO_0770.

Structurei

3D structure databases

ProteinModelPortaliP82177.
SMRiP82177. Positions 1-310.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
KOiK00024.
OMAiVEVKGFA.
OrthoDBiEOG6091FG.
PhylomeDBiP82177.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01516. Malate_dehydrog_1.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P82177-1 [UniParc]FASTAAdd to Basket

« Hide

MKVAVLGAAG GIGQALALLL KTQLPAGSKL SLYDIAPVTP GVAVDLSHIP    50
TAVEIKGFAG EDPTPALVGA DVVLISAGVA RKPGMDRSDL FNINAGIVRN 100
LIEKVAVTCP KALVGIITNP VNTTVAIAAE VMKKAGVYDK NRLFGVTTLD 150
VIRSETFIAE LKGLNVADVK INVIGGHSGV TILPLLSQVE GVTFSDEEVA 200
SLTKRIQNAG TEVVEAKAGG GSATLSMGQA ACRFGMSLVR GLQGEANVVE 250
CAYVDGGSEH AEFFAQPVLL GKNGIEKVLP YGEVSAFEAN ARDSMLDTLK 300
GDIKLGVDFV K 311
Length:311
Mass (Da):32,137
Last modified:November 15, 2002 - v2
Checksum:i9D3F5E86A2500A1B
GO

Mass spectrometryi

Molecular mass is 1734.8 Da from positions 3 - 21. Determined by ESI. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131Missing AA sequence 1 Publication
Sequence conflicti16 – 161L → LG AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN53846.1.
RefSeqiNP_716401.1. NC_004347.2.
WP_011071073.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN53846; AAN53846; SO_0770.
GeneIDi1168625.
KEGGison:SO_0770.
PATRICi23521213. VBISheOne101494_0740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN53846.1 .
RefSeqi NP_716401.1. NC_004347.2.
WP_011071073.1. NC_004347.2.

3D structure databases

ProteinModelPortali P82177.
SMRi P82177. Positions 1-310.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 211586.SO_0770.

Proteomic databases

PRIDEi P82177.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN53846 ; AAN53846 ; SO_0770 .
GeneIDi 1168625.
KEGGi son:SO_0770.
PATRICi 23521213. VBISheOne101494_0740.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000213792.
KOi K00024.
OMAi VEVKGFA.
OrthoDBi EOG6091FG.
PhylomeDBi P82177.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01516. Malate_dehydrog_1.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.
  2. "Automated nanoflow liquid chromatography/tandem mass spectrometric identification of proteins from Shewanella putrefaciens separated by two-dimensional polyacrylamide gel electrophoresis."
    Devreese B., Vanrobaeys F., Van Beeumen J.
    Rapid Commun. Mass Spectrom. 15:50-56(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-21, MASS SPECTROMETRY.
    Strain: MR-1.

Entry informationi

Entry nameiMDH_SHEON
AccessioniPrimary (citable) accession number: P82177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 15, 2002
Last modified: September 3, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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