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P82176 (IMPI_GALME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inducible metalloproteinase inhibitor protein

Cleaved into the following chain:

  1. IMPI alpha
Gene names
Name:IMPI
OrganismGalleria mellonella (Greater wax moth)
Taxonomic identifier7137 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPyraloideaPyralidaeGalleriinaeGalleria

Protein attributes

Sequence length170 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits thermolysin, bacillolysin and pseudolysin, B.polymyxa metalloprotease and human MMP1 and MMP3. No activity on trypsin or cysteine protease papain. Ref.1 Ref.3

Induction

During humoral immune response. By lipopolysaccharide (LPS). Ref.1 Ref.3

Post-translational modification

Cleaved Probable.

Five disulfide bonds are present. When artificially cleaved by thermolysin between Asn-56 and Ile-57, the two obtained chains (called heavy and light chains) remain linked.

The N-terminus is blocked.

Mass spectrometry

Molecular mass is 8360 Da from positions 20 - 88. Determined by MALDI. Ref.4

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 170151Inducible metalloproteinase inhibitor protein
PRO_0000021511
Chain20 – 8869IMPI alpha
PRO_0000021512

Sites

Site88 – 892Cleavage Probable

Amino acid modifications

Glycosylation481N-linked (GlcNAc...)
Glycosylation1491N-linked (GlcNAc...) Potential

Secondary structure

............... 170
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P82176 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: 1A5B272846AD129A

FASTA17018,759
        10         20         30         40         50         60 
MKCLLYLCLW CYCVLVSSSI VLICNGGHEY YECGGACDNV CADLHIQNKT NCPIINIRCN 

        70         80         90        100        110        120 
DKCYCEDGYA RDVNGKCIPI KDCPKIRSRR SIGIPVDKKC CTGPNEHYDE EKVSCPPETC 

       130        140        150        160        170 
ISLVAKFSCI DSPPPSPGCS CNSGYLRLNL TSPCIPICDC PQMQHSPDCQ 

« Hide

References

[1]"Cloning and expression of an inhibitor of microbial metalloproteinases from insects contributing to innate immunity."
Clermont A., Wedde M., Seitz V., Podsiadlowski L., Lenze D., Hummel M., Vilcinskas A.
Biochem. J. 382:315-322(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
[2]"Hemolymph proteins of the greater wax moth, Galleria mellonella."
Weise C., Bender O., Kopacek P., Hucho F.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Purification and characterization of an inducible metalloprotease inhibitor from the hemolymph of greater wax moth larvae, Galleria mellonella."
Wedde M., Weise C., Kopacek P., Franke P., Vilcinskas A.
Eur. J. Biochem. 255:535-543(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 57-82, FUNCTION, INDUCTION, BLOCKAGE OF N-TERMINUS, GLYCOSYLATION.
Tissue: Larval hemolymph.
[4]Weise C.
Submitted (JUL-2003) to UniProtKB
Cited for: SEQUENCE REVISION TO 63, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY330624 mRNA. Translation: AAQ73240.1.
AJ577749 mRNA. Translation: CAE12200.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3SSBX-ray1.80C/D19-56[»]
I/J57-88[»]
ProteinModelPortalP82176.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI08.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002919. TIL_dom.
[Graphical view]
PfamPF01826. TIL. 1 hit.
[Graphical view]
SUPFAMSSF57567. SSF57567. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP82176.

Entry information

Entry nameIMPI_GALME
AccessionPrimary (citable) accession number: P82176
Secondary accession number(s): Q67FQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 15, 2003
Last modified: April 16, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references