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P82176

- IMPI_GALME

UniProt

P82176 - IMPI_GALME

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Protein

Inducible metalloproteinase inhibitor protein

Gene

IMPI

Organism
Galleria mellonella (Greater wax moth)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits thermolysin, bacillolysin and pseudolysin, B.polymyxa metalloprotease and human MMP1 and MMP3. No activity on trypsin or cysteine protease papain.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei88 – 892CleavageCurated

GO - Molecular functioni

  1. metalloendopeptidase inhibitor activity Source: UniProtKB

GO - Biological processi

  1. anatomical structure morphogenesis Source: UniProtKB
  2. extracellular matrix organization Source: UniProtKB
  3. negative regulation of endopeptidase activity Source: GOC
  4. wound healing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Protein family/group databases

MEROPSiI08.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Inducible metalloproteinase inhibitor protein
Cleaved into the following chain:
Gene namesi
Name:IMPI
OrganismiGalleria mellonella (Greater wax moth)
Taxonomic identifieri7137 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPyraloideaPyralidaeGalleriinaeGalleria

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 170151Inducible metalloproteinase inhibitor proteinPRO_0000021511Add
BLAST
Chaini20 – 8869IMPI alphaPRO_0000021512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi48 – 481N-linked (GlcNAc...)1 Publication
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Cleaved.Curated
Five disulfide bonds are present. When artificially cleaved by thermolysin between Asn-56 and Ile-57, the two obtained chains (called heavy and light chains) remain linked.
The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

During humoral immune response. By lipopolysaccharide (LPS).2 Publications

Structurei

Secondary structure

1
170
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 349Combined sources
Helixi41 – 433Combined sources
Turni44 – 463Combined sources
Beta strandi49 – 513Combined sources
Beta strandi60 – 656Combined sources
Beta strandi69 – 713Combined sources
Beta strandi77 – 793Combined sources
Helixi80 – 823Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SSBX-ray1.80C/D19-56[»]
I/J57-88[»]
ProteinModelPortaliP82176.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82176.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR002919. TIL_dom.
[Graphical view]
PfamiPF01826. TIL. 1 hit.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82176-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKCLLYLCLW CYCVLVSSSI VLICNGGHEY YECGGACDNV CADLHIQNKT
60 70 80 90 100
NCPIINIRCN DKCYCEDGYA RDVNGKCIPI KDCPKIRSRR SIGIPVDKKC
110 120 130 140 150
CTGPNEHYDE EKVSCPPETC ISLVAKFSCI DSPPPSPGCS CNSGYLRLNL
160 170
TSPCIPICDC PQMQHSPDCQ
Length:170
Mass (Da):18,759
Last modified:August 15, 2003 - v2
Checksum:i1A5B272846AD129A
GO

Mass spectrometryi

Molecular mass is 8360 Da from positions 20 - 88. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330624 mRNA. Translation: AAQ73240.1.
AJ577749 mRNA. Translation: CAE12200.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330624 mRNA. Translation: AAQ73240.1 .
AJ577749 mRNA. Translation: CAE12200.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3SSB X-ray 1.80 C/D 19-56 [» ]
I/J 57-88 [» ]
ProteinModelPortali P82176.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi I08.006.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P82176.

Family and domain databases

InterProi IPR002919. TIL_dom.
[Graphical view ]
Pfami PF01826. TIL. 1 hit.
[Graphical view ]
SUPFAMi SSF57567. SSF57567. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of an inhibitor of microbial metalloproteinases from insects contributing to innate immunity."
    Clermont A., Wedde M., Seitz V., Podsiadlowski L., Lenze D., Hummel M., Vilcinskas A.
    Biochem. J. 382:315-322(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
  2. "Hemolymph proteins of the greater wax moth, Galleria mellonella."
    Weise C., Bender O., Kopacek P., Hucho F.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Purification and characterization of an inducible metalloprotease inhibitor from the hemolymph of greater wax moth larvae, Galleria mellonella."
    Wedde M., Weise C., Kopacek P., Franke P., Vilcinskas A.
    Eur. J. Biochem. 255:535-543(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 57-82, FUNCTION, INDUCTION, BLOCKAGE OF N-TERMINUS, GLYCOSYLATION.
    Tissue: Larval hemolymph.
  4. Weise C.
    Submitted (JUL-2003) to UniProtKB
    Cited for: SEQUENCE REVISION TO 63, MASS SPECTROMETRY.

Entry informationi

Entry nameiIMPI_GALME
AccessioniPrimary (citable) accession number: P82176
Secondary accession number(s): Q67FQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 15, 2003
Last modified: November 26, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3