Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inducible metalloproteinase inhibitor protein

Gene

IMPI

Organism
Galleria mellonella (Greater wax moth)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits thermolysin, bacillolysin and pseudolysin, B.polymyxa metalloprotease and human MMP1 and MMP3. No activity on trypsin or cysteine protease papain.2 Publications

GO - Molecular functioni

  • metalloendopeptidase inhibitor activity Source: UniProtKB

GO - Biological processi

  • anatomical structure morphogenesis Source: UniProtKB
  • extracellular matrix organization Source: UniProtKB
  • wound healing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Protein family/group databases

MEROPSiI08.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Inducible metalloproteinase inhibitor protein
Cleaved into the following chain:
Gene namesi
Name:IMPI
OrganismiGalleria mellonella (Greater wax moth)
Taxonomic identifieri7137 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaPyraloideaPyralidaeGalleriinaeGalleria

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002151120 – 170Inducible metalloproteinase inhibitor proteinAdd BLAST151
ChainiPRO_000002151220 – 88IMPI alphaAdd BLAST69

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi48N-linked (GlcNAc...)1 Publication1
Glycosylationi149N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Cleaved.Curated
Five disulfide bonds are present. When artificially cleaved by thermolysin between Asn-56 and Ile-57, the two obtained chains (called heavy and light chains) remain linked.
The N-terminus is blocked.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei88 – 89CleavageCurated2

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

During humoral immune response. By lipopolysaccharide (LPS).2 Publications

Structurei

Secondary structure

1170
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 34Combined sources9
Helixi41 – 43Combined sources3
Turni44 – 46Combined sources3
Beta strandi49 – 51Combined sources3
Beta strandi60 – 65Combined sources6
Beta strandi69 – 71Combined sources3
Beta strandi77 – 79Combined sources3
Helixi80 – 82Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SSBX-ray1.80C/D19-56[»]
I/J57-88[»]
ProteinModelPortaliP82176.
SMRiP82176.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82176.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR002919. TIL_dom.
[Graphical view]
PfamiPF01826. TIL. 1 hit.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCLLYLCLW CYCVLVSSSI VLICNGGHEY YECGGACDNV CADLHIQNKT
60 70 80 90 100
NCPIINIRCN DKCYCEDGYA RDVNGKCIPI KDCPKIRSRR SIGIPVDKKC
110 120 130 140 150
CTGPNEHYDE EKVSCPPETC ISLVAKFSCI DSPPPSPGCS CNSGYLRLNL
160 170
TSPCIPICDC PQMQHSPDCQ
Length:170
Mass (Da):18,759
Last modified:August 15, 2003 - v2
Checksum:i1A5B272846AD129A
GO

Mass spectrometryi

Molecular mass is 8360 Da from positions 20 - 88. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330624 mRNA. Translation: AAQ73240.1.
AJ577749 mRNA. Translation: CAE12200.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY330624 mRNA. Translation: AAQ73240.1.
AJ577749 mRNA. Translation: CAE12200.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SSBX-ray1.80C/D19-56[»]
I/J57-88[»]
ProteinModelPortaliP82176.
SMRiP82176.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI08.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP82176.

Family and domain databases

InterProiIPR002919. TIL_dom.
[Graphical view]
PfamiPF01826. TIL. 1 hit.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIMPI_GALME
AccessioniPrimary (citable) accession number: P82176
Secondary accession number(s): Q67FQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 15, 2003
Last modified: November 2, 2016
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.