P82172 (DLP1_ORNAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Defensin-like peptide 1 Short name=DLP-1 Alternative name(s): Ornithorhynchus venom defensin-like peptide C Short name=OvDLP-C
Organism	Ornithorhynchus anatinus (Duckbill platypus) [Reference proteome]
Taxonomic identifier	9258 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Prototheria › Monotremata › Ornithorhynchidae › Ornithorhynchus

Protein attributes

Sequence length	66 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Does not show antimicrobial, myotoxic, hemolytic and cell-promoting activities. Ref.2
Subcellular location	Secreted Ref.2.
Tissue specificity	Produced by the crural gland and detected in venom from the spur located on each male hind leg. Is also widely expressed in both male and female tissues, including intestine, kidney, spleen and testis. Ref.1 Ref.2

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Toxin
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from expression pattern Ref.2. Source: UniProtKB
Cellular_component	extracellular region Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Chain

25 – 66

42

Defensin-like peptide 1

PRO_0000079932

Amino acid modifications

Disulfide bond

33 ↔ 63

Disulfide bond

40 ↔ 56

Disulfide bond

48 ↔ 64

Experimental info

Sequence conflict

60

1

E → G AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

66

Helix Strand Turn

Details...

Helix

34 – 36

3

Beta strand

39 – 42

4

Beta strand

46 – 48

3

Beta strand

57 – 64

8

Sequences

	Sequence		Length	Mass (Da)
	P82172 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: C338407A4788CB68	FASTA	66	7,518
	10 20 30 40 50 60 MRLAYLLLLL VAVLFQAGGG SVEAFVQHRP RDCESINGVC RHKDTVNCRE IFLADCYNDE QKCCRK

References

[1]

"Expression patterns of platypus defensin and related venom genes across a range of tissue types reveal the possibility of broader functions for OvDLPs than previously suspected." Whittington C.M., Papenfuss A.T., Kuchel P.W., Belov K. Toxicon 52:559-565(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

[2]

"Solution structure of a defensin-like peptide from platypus venom." Torres A.M., Wang X., Fletcher J.I., Alewood D., Alewood P.F., Smith R., Simpson R.J., Nicholson G.M., Sutherland S.K., Gallagher C.H., King G.F., Kuchel P.W. Biochem. J. 341:785-794(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-66, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, SYNTHESIS, STRUCTURE BY NMR OF 25-66. Tissue: Venom and Venom gland.

Web resources

Platypus resources

Protein Spotlight

Platypus poison - Issue 29 of December 2002

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B8W	NMR	-	A	25-66	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSOANT00000002833; ENSOANP00000002832; ENSOANG00000001780.

Phylogenomic databases

GeneTree

ENSGT00420000030416.

HOVERGEN

HBG107815.

Family and domain databases

Gene3D

2.20.20.10. 1 hit.

InterPro

IPR012553. Defensin_3.
IPR023355. Myo_neuro_toxin.
[Graphical view]

Pfam

PF08131. Defensin_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P82172.

Entry information

Entry name

DLP1_ORNAN

Accession

Primary (citable) accession number: P82172

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	October 14, 2008
Last modified:	April 3, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program

Annotation program

Chordata Protein Annotation Program