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P82140 (DLP2_ORNAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Defensin-like peptide 2/4

Short name=DLP-2/DLP-4
Alternative name(s):
Ornithorhynchus venom defensin-like peptide B
Short name=OvDLP-B
OrganismOrnithorhynchus anatinus (Duckbill platypus) [Reference proteome]
Taxonomic identifier9258 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMonotremataOrnithorhynchidaeOrnithorhynchus

Protein attributes

Sequence length66 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Does not show antimicrobial, myotoxic, hemolytic and cell-promoting activities. Ref.2

Subcellular location

Secreted Ref.2.

Tissue specificity

Produced by the crural gland and detected in venom from the spur located on each male hind leg. Is also widely expressed in both male and female tissues, including brain, intestine, kidney, lung, spleen and testis. Ref.1 Ref.2 Ref.4

Post-translational modification

Stereoinversion of L-Met-26 (in DLP-4) to D-Met-26 (in DLP-2).

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionToxin
   PTMD-amino acid
Disulfide bond
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from expression pattern Ref.2. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from direct assay Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 242
PRO_0000352673
Chain25 – 6642Defensin-like peptide 2/4
PRO_0000079933

Amino acid modifications

Modified residue261D-methionine; in form DLP-2 Ref.4
Disulfide bond33 ↔ 63
Disulfide bond40 ↔ 56
Disulfide bond48 ↔ 64

Secondary structure

.......... 66
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P82140 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: 1825CB894D077B7B

FASTA667,599
        10         20         30         40         50         60 
MRLAYLLLLL VAVLFQAGGG SAKPIMFFEM QACWSHSGVC RDKSERNCKP MAWTYCENRN 


QKCCEY 

« Hide

References

[1]"Expression patterns of platypus defensin and related venom genes across a range of tissue types reveal the possibility of broader functions for OvDLPs than previously suspected."
Whittington C.M., Papenfuss A.T., Kuchel P.W., Belov K.
Toxicon 52:559-565(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Solution structure of a defensin-like peptide from platypus venom."
Torres A.M., Wang X., Fletcher J.I., Alewood D., Alewood P.F., Smith R., Simpson R.J., Nicholson G.M., Sutherland S.K., Gallagher C.H., King G.F., Kuchel P.W.
Biochem. J. 341:785-794(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-66, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, STRUCTURE BY NMR OF 25-66.
Tissue: Venom and Venom gland.
[3]"Defensin-like peptide-2 from platypus venom: member of a class of peptides with a distinct structural fold."
Torres A.M., de Plater G.M., Doverskog M., Birinyi-Strachan L.C., Nicholson G.M., Gallagher C.H., Kuchel P.W.
Biochem. J. 348:649-656(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-66, STRUCTURE BY NMR OF 25-66.
Tissue: Venom.
[4]"D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties."
Torres A.M., Tsampazi C., Geraghty D.P., Bansal P.S., Alewood P.F., Kuchel P.W.
Biochem. J. 391:215-220(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: D-AMINO ACID AT MET-26, TISSUE SPECIFICITY, STRUCTURE BY NMR OF 25-66.

Web resources

Platypus resources
Protein Spotlight

Platypus poison - Issue 29 of December 2002

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D6BNMR-A25-66[»]
1ZUENMR-A25-66[»]
1ZUFNMR-A25-66[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSOANT00000002832; ENSOANP00000002831; ENSOANG00000001779.

Phylogenomic databases

HOVERGENHBG107815.

Family and domain databases

Gene3D2.20.20.10. 1 hit.
InterProIPR012553. Defensin_3.
IPR023355. Myo_neuro_toxin.
[Graphical view]
PfamPF08131. Defensin_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP82140.

Entry information

Entry nameDLP2_ORNAN
AccessionPrimary (citable) accession number: P82140
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 14, 2008
Last modified: February 19, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Relevant documents

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references