Reviewed, UniProtKB/Swiss-Prot P82140 (DLP2_ORNAN)

Names and origin

Protein names	Recommended name:     Defensin-like peptide 2/4       Short name=DLP-2/DLP-4 Alternative name(s):     Ornithorhynchus venom defensin-like peptide B       Short name=OvDLP-B
Organism	Ornithorhynchus anatinus (Duckbill platypus)
Taxonomic identifier	9258 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Monotremata › Ornithorhynchidae › Ornithorhynchus

Protein attributes

Sequence length	66 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Does not show antimicrobial, myotoxic, hemolytic and cell-promoting activities.
Subcellular location	Secreted.
Tissue specificity	Produced by the crural gland and detected in venom from the spur located on each male hind leg. Is also widely expressed in both male and female tissues, including brain, intestine, kidney, lung, spleen and testis.
Post-translational modification	Stereoinversion of L-Met-26 (in DLP-4) to D-Met-26 (in DLP-2).

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Toxin
PTM	D-amino acid
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Ref.2 Inferred from expression pattern. Source: UniProtKB
Cellular component	extracellular region Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Potential

Propeptide

23 – 24

2

PRO_0000352673

Chain

25 – 66

42

Defensin-like peptide 2/4

PRO_0000079933

Amino acid modifications

Modified residue

26

1

D-methionine; in form DLP-2

Disulfide bond

33 ↔ 63

Disulfide bond

40 ↔ 56

Disulfide bond

48 ↔ 64

Secondary structure

1

.

.

.

.

.

.

.

66

Helix Strand Turn

Details...

Helix

33 – 36

4

Beta strand

39 – 42

4

Beta strand

61 – 64

4

Sequences

	Sequence		Length	Mass (Da)
	P82140-1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 1825CB894D077B7B	FASTA	66	7,599
	10 20 30 40 50 60 MRLAYLLLLL VAVLFQAGGG SAKPIMFFEM QACWSHSGVC RDKSERNCKP MAWTYCENRN QKCCEY

References

[1]	"Expression patterns of platypus defensin and related venom genes across a range of tissue types reveal the possibility of broader functions for OvDLPs than previously suspected." Whittington C.M., Papenfuss A.T., Kuchel P.W., Belov K. Toxicon 52:559-565(2008) [PubMed: 18662710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Solution structure of a defensin-like peptide from platypus venom." Torres A.M., Wang X., Fletcher J.I., Alewood D., Alewood P.F., Smith R., Simpson R.J., Nicholson G.M., Sutherland S.K., Gallagher C.H., King G.F., Kuchel P.W. Biochem. J. 341:785-794(1999) [PubMed: 10417345] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-66, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, STRUCTURE BY NMR OF 25-66. Tissue: Venom and Venom gland.
[3]	"Defensin-like peptide-2 from platypus venom: member of a class of peptides with a distinct structural fold." Torres A.M., de Plater G.M., Doverskog M., Birinyi-Strachan L.C., Nicholson G.M., Gallagher C.H., Kuchel P.W. Biochem. J. 348:649-656(2000) [PubMed: 10839998] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-66, STRUCTURE BY NMR OF 25-66. Tissue: Venom.
[4]	"D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties." Torres A.M., Tsampazi C., Geraghty D.P., Bansal P.S., Alewood P.F., Kuchel P.W. Biochem. J. 391:215-220(2005) [PubMed: 16033333] [Abstract] Cited for: D-AMINO ACID AT MET-26, TISSUE SPECIFICITY, STRUCTURE BY NMR OF 25-66.

Web resources

Platypus resources

Protein Spotlight

Platypus poison - Issue 29 of December 2002

Cross-references

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D6B	NMR	-	A	25-66	[»]
1ZUE	NMR	-	A	25-66	[»]
1ZUF	NMR	-	A	25-66	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSOANG00000001779. Ornithorhynchus anatinus. [Contig view]

Phylogenomic databases

HOVERGEN

P82140.

Family and domain databases

InterPro

IPR012553. Defensin_3.
[Graphical view]

Pfam

PF08131. Defensin_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DLP2_ORNAN

Accession

Primary (citable) accession number: P82140

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	October 14, 2008
Last modified:	October 14, 2008
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)