Reviewed, UniProtKB/Swiss-Prot P81943 (ALL5_APIGR)

Names and origin

Protein names	Recommended name:     Allergen Api g 5 Alternative name(s):     Allergen=Api g 5
Organism	Apium graveolens (Celery)
Taxonomic identifier	4045 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiaceae › Apioideae › apioid superclade › Apium clade › Apium

Protein attributes

Sequence length	86 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	FAD By similarity. UniProtKB P93479
Post-translational modification	Carries MUXF and MMXF, two complex N-linked glycans with alpha-1,3-fucose and beta-1,2-xylose residues in their structures. MMXF is added to Asn-62. Ref.2
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. The glycan moiety seems to constitute the relevant allergenic epitope. Ref.1 Ref.2
Sequence similarities	Belongs to the oxygen-dependent FAD-linked oxidoreductase family.
Caution	The order of the peptides shown is unknown. Ref.2

Ontologies

Keywords
Disease	Allergen
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW type I hypersensitivity Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›86	›86	Allergen Api g 5		PRO_0000128175
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Ref.2
Experimental info
	Non-adjacent residues	22 – 23	2
	Non-adjacent residues	52 – 53	2
	Non-adjacent residues	76 – 77	2
	Non-terminal residue	86	1

Sequences

	Sequence		Length	Mass (Da)
	P81943-1 [UniParc]. Last modified July 25, 2003. Version 3. Checksum: 09F5C64FA7ACD452	FASTA	86	9,441
	10 20 30 40 50 60 LPNPSGFVTC LSSISKSVYT PAINLKAVIA DPVAKTAVVQ AGATLGEVYY XIIYARVLWV 70 80 GNTTQKLEWI RSLHDYQSSF FPFFSA

References

[1]

"N-terminal sequences of high molecular weight allergens from celery tuber." Ganglberger E., Radauer C., Grimm R., Hoffmann-Sommergruber K., Breiteneder H., Scheiner O., Jensen-Jarolim E. Clin. Exp. Allergy 30:566-570(2000) [PubMed: 10718855] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-18, ALLERGENICITY. Tissue: Tuber.

[2]

"Cross-reactive N-glycans of Api g 5, a high molecular weight glycoprotein allergen from celery, are required for immunoglobulin E binding and activation of effector cells from allergic patients." Bublin M., Radauer C., Wilson I.B.H., Kraft D., Scheiner O., Breiteneder H., Hoffmann-Sommergruber K. FASEB J. 17:1697-1699(2003) [PubMed: 12958180] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-86, SEQUENCE REVISION, GLYCOSYLATION, ALLERGENICITY. Tissue: Tuber.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ALL5_APIGR

Accession

Primary (citable) accession number: P81943

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	July 25, 2003
Last modified:	November 25, 2008
This is version 32 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)