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Protein

Defensin-1

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Possesses antifungal activity sensitive to inorganic cations.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-1
Alternative name(s):
Antifungal protein Psd1
Defense-related peptide 1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4646Defensin-1PRO_0000074242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 461 Publication
Disulfide bondi14 ↔ 351 Publication
Disulfide bondi20 ↔ 401 Publication
Disulfide bondi24 ↔ 421 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Epidermis and vascular bundles of pods, stems, roots, leaves and wet or dry seeds.

Developmental stagei

Mature seed in dormancy.

Structurei

Secondary structure

1
46
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Beta strandi8 – 103Combined sources
Helixi17 – 2711Combined sources
Beta strandi33 – 364Combined sources
Beta strandi39 – 457Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKZNMR-A1-46[»]
2IIWmodel-A1-46[»]
2IJ1model-A1-46[»]
2IJTmodel-A1-46[»]
2IJUmodel-A1-46[»]
2IJVmodel-A1-46[»]
2IJWmodel-A1-46[»]
2IKLmodel-A1-46[»]
2IKMmodel-A1-46[»]
2IKNmodel-A1-46[»]
2IKPmodel-A1-46[»]
2IKRmodel-A1-46[»]
2IKTmodel-A1-46[»]
2IKVmodel-A1-46[»]
2IKWmodel-A1-46[»]
2IKXmodel-A1-46[»]
2IKYmodel-A1-46[»]
2IKZmodel-A1-46[»]
2IL0model-A1-46[»]
2ILCmodel-A1-46[»]
2ILDmodel-A1-46[»]
2ILEmodel-A1-46[»]
2ILFmodel-A1-46[»]
2ILGmodel-A1-46[»]
2ILHmodel-A1-46[»]
2ILJmodel-A1-46[»]
ProteinModelPortaliP81929.
SMRiP81929. Positions 1-46.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81929.

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P81929-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
KTCEHLADTY RGVCFTNASC DDHCKNKAHL ISGTCHNWKC FCTQNC
Length:46
Mass (Da):5,209
Last modified:March 5, 2002 - v2
Checksum:i12B4ABCF0A6701FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431T → G AA sequence (PubMed:11697857).Curated

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKZNMR-A1-46[»]
2IIWmodel-A1-46[»]
2IJ1model-A1-46[»]
2IJTmodel-A1-46[»]
2IJUmodel-A1-46[»]
2IJVmodel-A1-46[»]
2IJWmodel-A1-46[»]
2IKLmodel-A1-46[»]
2IKMmodel-A1-46[»]
2IKNmodel-A1-46[»]
2IKPmodel-A1-46[»]
2IKRmodel-A1-46[»]
2IKTmodel-A1-46[»]
2IKVmodel-A1-46[»]
2IKWmodel-A1-46[»]
2IKXmodel-A1-46[»]
2IKYmodel-A1-46[»]
2IKZmodel-A1-46[»]
2IL0model-A1-46[»]
2ILCmodel-A1-46[»]
2ILDmodel-A1-46[»]
2ILEmodel-A1-46[»]
2ILFmodel-A1-46[»]
2ILGmodel-A1-46[»]
2ILHmodel-A1-46[»]
2ILJmodel-A1-46[»]
ProteinModelPortaliP81929.
SMRiP81929. Positions 1-46.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP81929.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of two novel defense peptides from pea (Pisum sativum) seeds."
    Almeida M.S., Cabral K.M., Zingali R.B., Kurtenbach E.
    Arch. Biochem. Biophys. 378:278-286(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Strain: cv. Mikado.
    Tissue: Epidermis and Seed endosperm.
  2. "cDNA cloning and heterologous expression of functional cysteine-rich antifungal protein Psd1 in the yeast Pichia pastoris."
    Almeida M.S., Cabral K.S., de Medeiros L.N., Valente A.P., Almeida F.C., Kurtenbach E.
    Arch. Biochem. Biophys. 395:199-207(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-23, DISULFIDE BONDS.
  3. "Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action."
    Almeida M.S., Cabral K.M., Kurtenbach E., Almeida F.C., Valente A.P.
    J. Mol. Biol. 315:749-757(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiDEF1_PEA
AccessioniPrimary (citable) accession number: P81929
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: June 8, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.