Reviewed, UniProtKB/Swiss-Prot P81921 (OR47A_DROME)

Names and origin

Protein names

Recommended name:
    Odorant receptor 47a

Gene names

Name:	Or47a
Synonyms:	AN10, dor24, DOR47E.1, Or47E.1
ORF Names:	CG13225

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complexes with Or83b to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide. Ref.6 Ref.7 Ref.8
Subunit structure	Interacts with Or83b. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway. Ref.6 Ref.7 Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed with Or83b in 40 olfactory receptor neurons in a broad area across the antenna, including both anterior and posterior faces. This expression pattern matches the distribution of the small sensilla basiconica. Expression in the antenna is observed late in antennal development at 93 hours APF. Ref.6 Ref.5
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Or83b complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the odorant-gated ionic channel family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Ref.1 Ref.5 Non-traceable author statement. Source: UniProtKB response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of smell Ref.1 Ref.5 Non-traceable author statement. Source: UniProtKB
Cellular component	integral to membrane Ref.1 Ref.5 Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Ref.1 Ref.5 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Odorant receptor 47a		PRO_0000174250
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 54	21	1 Potential
	Topological domain	55 – 62	8	Extracellular Potential
	Transmembrane	63 – 83	21	2 Potential
	Topological domain	84 – 129	46	Cytoplasmic Potential
	Transmembrane	130 – 150	21	3 Potential
	Topological domain	151 – 175	25	Extracellular Potential
	Transmembrane	176 – 196	21	4 Potential
	Topological domain	197 – 255	59	Cytoplasmic Potential
	Transmembrane	256 – 276	21	5 Potential
	Topological domain	277 – 284	8	Extracellular Potential
	Transmembrane	285 – 305	21	6 Potential
	Topological domain	306 – 357	52	Cytoplasmic Potential
	Transmembrane	358 – 378	21	7 Potential
	Topological domain	379 – 385	7	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	P81921-1 [UniParc]. Last modified August 1, 1999. Version 1. Checksum: 498DA16E50A86038	FASTA	385	43,966
	10 20 30 40 50 60 MDSFLQVQKS TIALLGFDLF SENREMWKRP YRAMNVFSIA AIFPFILAAV LHNWKNVLLL 70 80 90 100 110 120 ADAMVALLIT ILGLFKFSMI LYLRRDFKRL IDKFRLLMSN EAEQGEEYAE ILNAANKQDQ 130 140 150 160 170 180 RMCTLFRTCF LLAWALNSVL PLVRMGLSYW LAGHAEPELP FPCLFPWNIH IIRNYVLSFI 190 200 210 220 230 240 WSAFASTGVV LPAVSLDTIF CSFTSNLCAF FKIAQYKVVR FKGGSLKESQ ATLNKVFALY 250 260 270 280 290 300 QTSLDMCNDL NQCYQPIICA QFFISSLQLC MLGYLFSITF AQTEGVYYAS FIATIIIQAY 310 320 330 340 350 360 IYCYCGENLK TESASFEWAI YDSPWHESLG AGGASTSICR SLLISMMRAH RGFRITGYFF 370 380 EANMEAFSSI VRTAMSYITM LRSFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A spatial map of olfactory receptor expression in the Drosophila antenna." Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R. Cell 96:725-736(1999) [PubMed: 10089887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Oregon-R. Tissue: Antenna.
[2]	"Identification of candidate Drosophila olfactory receptors from genomic DNA sequence." Gao Q., Chess A. Genomics 60:31-39(1999) [PubMed: 10458908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A novel family of divergent seven-transmembrane proteins: candidate odorant receptors in Drosophila." Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R. Neuron 22:327-338(1999) [PubMed: 10069338] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[6]	"Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo." Benton R., Sachse S., Michnick S.W., Vosshall L.B. PLoS Biol. 4:E20-E20(2006) [PubMed: 16402857] [Abstract] Cited for: FUNCTION, INTERACTION WITH OR85B, TISSUE SPECIFICITY.
[7]	"Insect olfactory receptors are heteromeric ligand-gated ion channels." Sato K., Pellegrino M., Nakagawa T., Nakagawa T., Vosshall L.B., Touhara K. Nature 452:1002-1006(2008) [PubMed: 18408712] [Abstract] Cited for: FUNCTION, INTERACTION WITH OR85B.
[8]	"Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels." Wicher D., Schaefer R., Bauernfeind R., Stensmyr M.C., Heller R., Heinemann S.H., Hansson B.S. Nature 452:1007-1011(2008) [PubMed: 18408711] [Abstract] Cited for: FUNCTION, INTERACTION WITH OR85B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF156880 mRNA. Translation: AAD39006.1. AE013599 Genomic DNA. Translation: AAF58695.1.
RefSeq	NP_523689.1.
UniGene	Dm.2583
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P81921.
Genome annotation databases
Ensembl	FBgn0026386. Drosophila melanogaster. [Contig view]
GeneID	36187.
KEGG	dme:Dmel_CG13225.
NMPDR	fig|7227.3.peg.4595.
Organism-specific databases
FlyBase	FBgn0026386. Or47a.
Phylogenomic databases
HOGENOM	P81921.
OMA	P81921. EANMEAF.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-003985-MON.
Gene expression databases
ArrayExpress	P81921.
GermOnline	CG13225. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	797246.

Entry information

Entry name

OR47A_DROME

Accession

Primary (citable) accession number: P81921
Secondary accession number(s): Q9U6X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1999
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project