P81917 (OR43A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Odorant receptor 43a

Gene names

Name:	Or43a
Synonyms:	AN14, DOR43B.1, dor87, Or43B.1
ORF Names:	CG1854

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Complexes with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide. Ref.6
Subunit structure	Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway. Ref.6
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed with Orco in 20 sensory neurons on the distal edge of the antenna. Ref.1 Ref.5 Ref.6
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the heteromeric odorant receptor channel (TC 1.A.69) family. [View classification]

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Non-traceable author statement Ref.5Ref.1. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW odorant binding Traceable author statement. Source: FlyBase olfactory receptor activity Non-traceable author statement Ref.5Ref.1. Source: UniProtKB protein binding Inferred from physical interaction Ref.6. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Odorant receptor 43a		PRO_0000174244
Regions
	Topological domain	1 – 32	32	Cytoplasmic Potential
	Transmembrane	33 – 53	21	Helical; Name=1; Potential
	Topological domain	54 – 58	5	Extracellular Potential
	Transmembrane	59 – 79	21	Helical; Name=2; Potential
	Topological domain	80 – 123	44	Cytoplasmic Potential
	Transmembrane	124 – 144	21	Helical; Name=3; Potential
	Topological domain	145 – 178	34	Extracellular Potential
	Transmembrane	179 – 199	21	Helical; Name=4; Potential
	Topological domain	200 – 247	48	Cytoplasmic Potential
	Transmembrane	248 – 268	21	Helical; Name=5; Potential
	Topological domain	269 – 276	8	Extracellular Potential
	Transmembrane	277 – 297	21	Helical; Name=6; Potential
	Topological domain	298 – 342	45	Cytoplasmic Potential
	Transmembrane	343 – 363	21	Helical; Name=7; Potential
	Topological domain	364 – 376	13	Extracellular Potential
Experimental info
	Sequence conflict	154	1	L → V in AAD26361. Ref.1
	Sequence conflict	237 – 272	36	Missing Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P81917 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 1B3638FE7EA49606	FASTA	376	43,090
	10 20 30 40 50 60 MTIEDIGLVG INVRMWRHLA VLYPTPGSSW RKFAFVLPVT AMNLMQFVYL LRMWGDLPAF 70 80 90 100 110 120 ILNMFFFSAI FNALMRTWLV IIKRRQFEEF LGQLATLFHS ILDSTDEWGR GILRRAEREA 130 140 150 160 170 180 RNLAILNLSA SFLDIVGALV SPLFREERAH PFGLALPGVS MTSSPVYEVI YLAQLPTPLL 190 200 210 220 230 240 LSMMYMPFVS LFAGLAIFGK AMLQILVHRL GQIGGEEQSE EERFQRLASC IAYHTQVMRY 250 260 270 280 290 300 VWQLNKLVAN IVAVEAIIFG SIICSLLFCL NIITSPTQVI SIVMYILTML YVLFTYYNRA 310 320 330 340 350 360 NEICLENNRV AEAVYNVPWY EAGTRFRKTL LIFLMQTQHP MEIRVGNVYP MTLAMFQSLL 370 NASYSYFTML RGVTGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A spatial map of olfactory receptor expression in the Drosophila antenna." Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R. Cell 96:725-736(1999) [PubMed: 10089887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Oregon-R. Tissue: Antenna.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"Identification of candidate Drosophila olfactory receptors from genomic DNA sequence." Gao Q., Chess A. Genomics 60:31-39(1999) [PubMed: 10458908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-342.
[5]	"A novel family of divergent seven-transmembrane proteins: candidate odorant receptors in Drosophila." Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R. Neuron 22:327-338(1999) [PubMed: 10069338] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[6]	"Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo." Benton R., Sachse S., Michnick S.W., Vosshall L.B. PLoS Biol. 4:E20-E20(2006) [PubMed: 16402857] [Abstract] Cited for: FUNCTION, INTERACTION WITH OR85B, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF127926 mRNA. Translation: AAD26361.1. AE013599 Genomic DNA. Translation: AAF59280.1.
RefSeq	NP_523647.2. NM_078923.2.
UniGene	Dm.2588.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29175N.
STRING	P81917.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089051; FBpp0088122; FBgn0026389.
GeneID	35644.
KEGG	dme:Dmel_CG1854.
NMPDR	fig|7227.3.peg.3605.
Organism-specific databases
CTD	35644.
FlyBase	FBgn0026389. Or43a.
Phylogenomic databases
eggNOG	inNOG05706.
GeneTree	EMGT00050000009324.
InParanoid	P81917.
OMA	IFLMQTQ.
OrthoDB	EOG4573P9.
PhylomeDB	P81917.
Gene expression databases
Bgee	P81917.
GermOnline	CG1854. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
KO	K08471.
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	794402.

Entry information

Entry name

OR43A_DROME

Accession

Primary (citable) accession number: P81917
Secondary accession number(s): Q9U6X4, Q9V4J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project