Reviewed, UniProtKB/Swiss-Prot P81910 (OR22B_DROME)

Names and origin

Protein names

Recommended name:
    Odorant receptor 22b

Gene names

Name:	Or22b
Synonyms:	AN12, DOR22A.2, dor67, Or22A.2
ORF Names:	CG4231

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complexes with Or83b to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide. Ref.8
Subunit structure	Interacts with Or83b, via conserved C-terminal cytoplasmic loops. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway. Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed with Or83b in 20-22 sensory neurons on the medial-proximal edge of the antenna. This expression pattern matches the distribution of the large sensilla basiconica. Expression is first seen at 60 hours APF in a subset of cells restricted to a subregion of the developing antenna. Expression continues throughout antennal development. Ref.8 Ref.1 Ref.7
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Or83b complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the odorant-gated ionic channel family.
Sequence caution	The sequence CAR79088.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention and rearrangment of DNA.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Ref.1 Ref.7 Non-traceable author statement. Source: UniProtKB response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of smell Ref.1 Ref.7 Non-traceable author statement. Source: UniProtKB
Cellular component	dendrite Inferred from direct assay. Source: FlyBase integral to membrane Ref.1 Ref.7 Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Ref.1 Ref.7 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Odorant receptor 22b		PRO_0000174233
Regions
	Topological domain	1 – 49	49	Cytoplasmic Potential
	Transmembrane	50 – 70	21	1 Potential
	Topological domain	71 – 85	15	Extracellular Potential
	Transmembrane	86 – 105	20	2 Potential
	Topological domain	106 – 143	38	Cytoplasmic Potential
	Transmembrane	144 – 164	21	3 Potential
	Topological domain	165 – 194	30	Extracellular Potential
	Transmembrane	195 – 215	21	4 Potential
	Topological domain	216 – 268	53	Cytoplasmic Potential
	Transmembrane	269 – 289	21	5 Potential
	Topological domain	290 – 295	6	Extracellular Potential
	Transmembrane	296 – 316	21	6 Potential
	Topological domain	317 – 347	31	Cytoplasmic Potential
	Transmembrane	348 – 368	21	7 Potential
	Topological domain	369 – 397	29	Extracellular Potential
Natural variations
	Natural variant	3	1	S → G in strain: MA7. Ref.4
	Natural variant	4	1	Q → K in strain: LA116, MA18, MA45, MA48, MA50, MA7, MA8, MW25, MW29, MW3, MW32, MW37, MW44, MW46, MW56, MW6, MW7, MW9, NC21a, NC52a, NC62a, NC73a and NC79b. Ref.4
	Natural variant	25	1	V → I in strain: LA116, MA13, MA18, MA43, MA45, MA48, MA50, MA7, MA74, MA8, MW25, MW27, MW29, MW3, MW32, MW37, MW44, MW46, MW56, MW6, MW7, MW9, NC21a, NC52a, NC62a, NC73a and NC79b. Ref.4
	Natural variant	30	1	V → L in strain: NC52a and NC73a. Ref.4
	Natural variant	38	1	V → E in strain: NC21a, NC52a, NC62a, NC73a and NC79b. Ref.4
	Natural variant	45	1	D → I in strain: LA116, MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	47	1	H → P in strain: LA116. Ref.4
	Natural variant	69	1	V → A in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	87	1	I → L in strain: LA116. Ref.4
	Natural variant	88	1	Q → E in strain: LA116. Ref.4
	Natural variant	92	1	N → D in strain: NC21a, NC52a, NC62a, NC73a and NC79b. Ref.4
	Natural variant	93	1	M → V in strain: LA116. Ref.4
	Natural variant	101	1	Y → C in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	102	1	L → F in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	111	1	Q → E in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	126	1	C → R in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	140	1	G → A in strain: LA9. Ref.4
	Natural variant	150	1	A → L in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	156	1	I → F in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	157	1	S → T in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	167	1	I → V in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	194	1	R → M in strain: LA116, MA13, MA18, MA43, MA45, MA48, MA50, MA7, MA74, MA8, MW25, MW27, MW29, MW3, MW32, MW37, MW44, MW46, MW56, MW6, MW7, MW9, NC21a, NC52a, NC62a, NC73a and NC79b. Ref.4
	Natural variant	196	1	W → L in strain: LA116. Ref.4
	Natural variant	201	1	D → A in strain: NC21a, NC52a, NC62a, NC73a and NC79b. Ref.4
	Natural variant	213	1	V → L in strain: LA116, MA45, MA50, MW37, MW6, MW9, NC52a and NC73a. Ref.4
	Natural variant	214	1	I → M in strain: LA116, MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	220	1	T → S in strain: LA116, MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	235	1	R → M in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	245	1	A → T in strain: LA116, MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	256	1	D → N in strain: MW44. Ref.4
	Natural variant	267	1	G → R in strain: MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	278	1	I → V in strain: LA108 and LA120. Ref.4
	Natural variant	309	1	Q → E in strain: LA116, LA25, LA4, MA24, MA45, MA50, MW37, MW6 and MW9. Ref.4
	Natural variant	313	1	F → S in strain: NC16a. Ref.4
	Natural variant	341	1	R → S in strain: LA116. Ref.4
	Natural variant	354	1	L → I in strain: NC52a, NC73a and NC79b. Ref.4
	Natural variant	387	1	V → M in strain: LA9. Ref.4
Experimental info
	Sequence conflict	52 – 60	9	STFVTLLIF → LAFVNIVML in CAR79365. Ref.4
	Sequence conflict	58	1	L → V in AAD26359. Ref.1
	Sequence conflict	67 – 73	7	VSVEYIQ → ISIEYLH in CAR79365. Ref.4
	Sequence conflict	129 – 133	5	ERTIV → DRSII in CAR79363. Ref.4
	Sequence conflict	129 – 133	5	ERTIV → DRSII in CAR79362. Ref.4
	Sequence conflict	129 – 133	5	ERTIV → DRSII in CAR79361. Ref.4
	Sequence conflict	129 – 133	5	ERTIV → DRSII in CAR79351. Ref.4
	Sequence conflict	129 – 133	5	ERTIV → DRSII in CAR79350. Ref.4
	Sequence conflict	256	1	D → TICFDKFFYLPYFFS Ref.3
	Sequence conflict	376 – 397	22	MVKLA…AEKFQ → VSINQYEL Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P81910-1 [UniParc]. Last modified January 20, 2009. Version 3. Checksum: CE0CDA1C5EFE11DA	FASTA	397	46,402
	10 20 30 40 50 60 MLSQFFPHIK EKPLSERVKS RDAFVYLDRV MWSFGWTVPE NKRWDLHYKL WSTFVTLLIF 70 80 90 100 110 120 ILLPISVSVE YIQRFKTFSA GEFLSSIQIG VNMYGSSFKS YLTMMGYKKR QEAKMSLDEL 130 140 150 160 170 180 DKRCVCDEER TIVHRHVALG NFCYIFYHIA YTSFLISNFL SFIMKRIHAW RMYFPYVDPE 190 200 210 220 230 240 KQFYISSIAE VILRGWAVFM DLCTDVCPLI SMVIARCHIT LLKQRLRNLR SEPGRTEDEY 250 260 270 280 290 300 LKELADCVRD HRLILDYVDA LRSVFSGTIF VQFLLIGIVL GLSMINIMFF STLSTGVAVV 310 320 330 340 350 360 LFMSCVSMQT FPFCYLCNMI MDDCQEMADS LFQSDWTSAD RRYKSTLVYF LHNLQQPIIL 370 380 390 TAGGVFPISM QTNLNMVKLA FTVVTIVKQF NLAEKFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A spatial map of olfactory receptor expression in the Drosophila antenna." Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R. Cell 96:725-736(1999) [PubMed: 10089887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Oregon-R. Tissue: Antenna.
[2]	Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 58.
[3]	"Identification of candidate Drosophila olfactory receptors from genomic DNA sequence." Gao Q., Chess A. Genomics 60:31-39(1999) [PubMed: 10458908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Nucleotide and copy-number polymorphism at the odorant receptor genes Or22a and Or22b in Drosophila melanogaster." Aguade M. Mol. Biol. Evol. 26:61-70(2009) [PubMed: 18922763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLY-3; LYS-4; ILE-25; LEU-30; GLU-38; ILE-45; PRO-47; ALA-69; LEU-87; GLU-88; ASP-92; VAL-93; CYS-101; PHE-102; GLU-111; ARG-126; ALA-140; LEU-150; PHE-156; THR-157; VAL-167; MET-194; LEU-196; ALA-201; LEU-213; MET-214; SER-220; MET-235; THR-245; ASN-256; ARG-267; VAL-278; GLU-309; SER-313; SER-341; ILE-354 AND MET-387. Strain: CN1, CN13, CN14, CN18, CN21, CN28, CN29, CN3, CN43, CN45, CN46, CN51, LA108, LA116, LA120, LA128, LA13, LA15, LA25, LA3, LA32, LA34, LA35, LA4, LA9, MA13, MA18, MA21, MA24, MA43, MA45, MA48, MA50, MA7, MA74, MA8, MW25, MW27, MW29, MW3, MW32, MW37, MW44, MW46, MW56, MW6, MW7, MW9, NC100b, NC10b, NC16a, NC1b, NC21a, NC37a, NC52a, NC62a, NC73a, NC79b, NC84a, NC88a, NC89a and NC97a.
[5]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[6]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[7]	"A novel family of divergent seven-transmembrane proteins: candidate odorant receptors in Drosophila." Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R. Neuron 22:327-338(1999) [PubMed: 10069338] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY. Tissue: Antenna.
[8]	"Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo." Benton R., Sachse S., Michnick S.W., Vosshall L.B. PLoS Biol. 4:E20-E20(2006) [PubMed: 16402857] [Abstract] Cited for: FUNCTION, INTERACTION WITH OR83B, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF127924 mRNA. Translation: AAD26359.2. FM212142 Genomic DNA. Translation: CAR79042.1. FM212143 Genomic DNA. Translation: CAR79044.1. FM212144 Genomic DNA. Translation: CAR79046.1. FM212145 Genomic DNA. Translation: CAR79048.1. FM212146 Genomic DNA. Translation: CAR79050.1. FM212147 Genomic DNA. Translation: CAR79052.1. FM212148 Genomic DNA. Translation: CAR79054.1. FM212149 Genomic DNA. Translation: CAR79056.1. FM212150 Genomic DNA. Translation: CAR79058.1. FM212151 Genomic DNA. Translation: CAR79060.1. FM212152 Genomic DNA. Translation: CAR79062.1. FM212153 Genomic DNA. Translation: CAR79064.1. FM212154 Genomic DNA. Translation: CAR79066.1. FM212155 Genomic DNA. Translation: CAR79068.1. FM212156 Genomic DNA. Translation: CAR79070.1. FM212157 Genomic DNA. Translation: CAR79072.1. FM212158 Genomic DNA. Translation: CAR79074.1. FM212159 Genomic DNA. Translation: CAR79076.1. FM212160 Genomic DNA. Translation: CAR79078.1. FM212161 Genomic DNA. Translation: CAR79080.1. FM212162 Genomic DNA. Translation: CAR79082.1. FM212163 Genomic DNA. Translation: CAR79084.1. FM212164 Genomic DNA. Translation: CAR79086.1. FM212165 Genomic DNA. Translation: CAR79088.1. Sequence problems. FM212166 Genomic DNA. Translation: CAR79090.1. FM212167 Genomic DNA. Translation: CAR79092.1. FM212168 Genomic DNA. Translation: CAR79094.1. FM212169 Genomic DNA. Translation: CAR79096.1. FM212170 Genomic DNA. Translation: CAR79098.1. FM212171 Genomic DNA. Translation: CAR79100.1. FM212172 Genomic DNA. Translation: CAR79102.1. FM212173 Genomic DNA. Translation: CAR79104.1. FM212174 Genomic DNA. Translation: CAR79106.1. FM212175 Genomic DNA. Translation: CAR79108.1. FM212176 Genomic DNA. Translation: CAR79110.1. FM212177 Genomic DNA. Translation: CAR79112.1. FM212178 Genomic DNA. Translation: CAR79114.1. FM212180 Genomic DNA. Translation: CAR79340.1. FM212181 Genomic DNA. Translation: CAR79341.1. FM212182 Genomic DNA. Translation: CAR79342.1. FM212183 Genomic DNA. Translation: CAR79343.1. FM212184 Genomic DNA. Translation: CAR79344.1. FM212185 Genomic DNA. Translation: CAR79345.1. FM212186 Genomic DNA. Translation: CAR79346.1. FM212187 Genomic DNA. Translation: CAR79347.1. FM212188 Genomic DNA. Translation: CAR79348.1. FM212189 Genomic DNA. Translation: CAR79349.1. FM212190 Genomic DNA. Translation: CAR79350.1. FM212191 Genomic DNA. Translation: CAR79351.1. FM212192 Genomic DNA. Translation: CAR79352.1. FM212193 Genomic DNA. Translation: CAR79353.1. FM212194 Genomic DNA. Translation: CAR79354.1. FM212195 Genomic DNA. Translation: CAR79355.1. FM212196 Genomic DNA. Translation: CAR79356.1. FM212197 Genomic DNA. Translation: CAR79357.1. FM212198 Genomic DNA. Translation: CAR79358.1. FM212199 Genomic DNA. Translation: CAR79359.1. FM212200 Genomic DNA. Translation: CAR79360.1. FM212201 Genomic DNA. Translation: CAR79361.1. FM212202 Genomic DNA. Translation: CAR79362.1. FM212203 Genomic DNA. Translation: CAR79363.1. FM212205 Genomic DNA. Translation: CAR79365.1. AE014134 Genomic DNA. Translation: AAF51363.1.
RefSeq	NP_477425.1.
UniGene	Dm.4820
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P81910. 1 interaction.
Protein family/group databases
TCDB	1.A.69.1.1. heteromeric odorant receptor channel (HORC) family.
Proteomic databases
PRIDE	P81910.
Genome annotation databases
Ensembl	FBgn0026397. Drosophila melanogaster. [Contig view]
GeneID	33336.
KEGG	dme:Dmel_CG4231.
Organism-specific databases
FlyBase	FBgn0026397. Or22b.
Phylogenomic databases
HOGENOM	P81910.
Gene expression databases
ArrayExpress	P81910.
GermOnline	CG4231. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	783088.

Entry information

Entry name

OR22B_DROME

Accession

Primary (citable) accession number: P81910
Secondary accession number(s): B7VC06 , B7VC10, B7VC50, B7VC52, B7VC62, B7VC68, B7VC76, B7VC81, B7VC83, B7VC85, B7VC86, B7VC87, B7VC91, B7VC99, B7VCA1, B7VCA6, Q9U6X6, Q9VQ19

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 20, 2009
Last modified:	June 16, 2009
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project