P81909 (OR22A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Odorant receptor 22a

Gene names

Name:	Or22a
Synonyms:	AN11, DOR22A.1, dor53, Or22A.1
ORF Names:	CG12193

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Complexes with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide. Ref.7 Ref.8
Subunit structure	Interacts with Orco, via conserved C-terminal cytoplasmic loops. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway. Ref.7 Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed with Orco in 17-20 sensory neurons on the medial-proximal edge of the antenna. Ref.1 Ref.5 Ref.6 Ref.7
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the heteromeric odorant receptor channel (TC 1.A.69) family. [View classification]

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Non-traceable author statement Ref.6Ref.5. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell sensory dendrite Inferred from direct assay. Source: FlyBase
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Non-traceable author statement Ref.6Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ance-2	Q9VJV2	1	EBI-131262,EBI-119944
CG17134	Q9VKP6	1	EBI-131262,EBI-153918

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Odorant receptor 22a		PRO_0000174232
Regions
	Topological domain	1 – 49	49	Cytoplasmic Potential
	Transmembrane	50 – 70	21	Helical; Name=1; Potential
	Topological domain	71 – 86	16	Extracellular Potential
	Transmembrane	87 – 107	21	Helical; Name=2; Potential
	Topological domain	108 – 136	29	Cytoplasmic Potential
	Transmembrane	137 – 157	21	Helical; Name=3; Potential
	Topological domain	158 – 182	25	Extracellular Potential
	Transmembrane	183 – 203	21	Helical; Name=4; Potential
	Topological domain	204 – 263	60	Cytoplasmic Potential
	Transmembrane	264 – 280	17	Helical; Name=5; Potential
	Topological domain	281 – 286	6	Extracellular Potential
	Transmembrane	287 – 304	18	Helical; Name=6; Potential
	Topological domain	305 – 356	52	Cytoplasmic Potential
	Transmembrane	357 – 377	21	Helical; Name=7; Potential
	Topological domain	378 – 397	20	Extracellular Potential
Natural variations
	Natural variant	8	1	H → Y in strain: MA74. Ref.2
	Natural variant	59	1	M → I in strain: NC1b. Ref.2
	Natural variant	67	1	I → M in strain: MA43 and MA74. Ref.2
	Natural variant	93	1	M → V in strain: NC1b and NC97a. Ref.2
	Natural variant	100	1	C → S in strain: CN1, CN13, CN18, CN21, CN29, CN45, CN51, LA128, LA13, LA15, LA25, LA3, LA32, LA34, LA35, LA4, MA21, MA24, NC1b, NC100b, NC37a, NC84a, NC89a and NC97a. Ref.2
	Natural variant	102	1	F → L in strain: NC97a. Ref.2
	Natural variant	196	1	E → A in strain: CN1, CN13, CN18, CN21, CN28, CN29, CN45, CN51, LA120, LA128, LA13, LA15, LA25, LA3, LA32, LA34, LA35, LA4, LA6, LA9, MA21, MA24, NC1b, NC100b, NC16a, NC37a, NC84a, NC89a and NC97a. Ref.2
	Natural variant	201	1	D → E in strain: NC1b and NC97a. Ref.2
	Natural variant	220	1	S → N in strain: CN14. Ref.2
	Natural variant	292	1	T → S in strain: CN13. Ref.2
	Natural variant	328	1	S → A in strain: LA6. Ref.2
	Natural variant	329	1	N → D in strain: LA6. Ref.2
	Natural variant	330	1	C → S in strain: LA6. Ref.2
	Natural variant	359	1	T → I in strain: LA6. Ref.2
	Natural variant	375	1	A → N in strain: LA6. Ref.2
	Natural variant	382	1	S → T in strain: LA6. Ref.2
	Natural variant	385	1	T → A in strain: NC1b and NC97a. Ref.2
	Natural variant	386	1	V → I in strain: LA6. Ref.2
	Natural variant	387	1	I → V in strain: LA6. Ref.2
	Natural variant	395	1	R → K in strain: LA6. Ref.2
Experimental info
	Sequence conflict	256	1	D → SIPFLFINGLFLFLS Ref.1
	Sequence conflict	376 – 397	22	MVKLA…AERFQ → VGVNILDISLILHLF Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P81909 [UniParc]. Last modified August 1, 1999. Version 1. Checksum: E9D20EF06E69E883	FASTA	397	46,754
	10 20 30 40 50 60 MLSKFFPHIK EKPLSERVKS RDAFIYLDRV MWSFGWTEPE NKRWILPYKL WLAFVNIVML 70 80 90 100 110 120 ILLPISISIE YLHRFKTFSA GEFLSSLEIG VNMYGSSFKC AFTLIGFKKR QEAKVLLDQL 130 140 150 160 170 180 DKRCLSDKER STVHRYVAMG NFFDILYHIF YSTFVVMNFP YFLLERRHAW RMYFPYIDSD 190 200 210 220 230 240 EQFYISSIAE CFLMTEAIYM DLCTDVCPLI SMLMARCHIS LLKQRLRNLR SKPGRTEDEY 250 260 270 280 290 300 LEELTECIRD HRLLLDYVDA LRPVFSGTIF VQFLLIGTVL GLSMINLMFF STFWTGVATC 310 320 330 340 350 360 LFMFDVSMET FPFCYLCNMI IDDCQEMSNC LFQSDWTSAD RRYKSTLVYF LHNLQQPITL 370 380 390 TAGGVFPISM QTNLAMVKLA FSVVTVIKQF NLAERFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of candidate Drosophila olfactory receptors from genomic DNA sequence." Gao Q., Chess A. Genomics 60:31-39(1999) [PubMed: 10458908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: Berkeley.
[2]	"Nucleotide and copy-number polymorphism at the odorant receptor genes Or22a and Or22b in Drosophila melanogaster." Aguade M. Mol. Biol. Evol. 26:61-70(2009) [PubMed: 18922763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TYR-8; ILE-59; MET-67; VAL-93; SER-100; LEU-102; ALA-196; GLU-201; ASN-220; SER-292; ALA-328; ASP-329; SER-330; ILE-359; ASN-375; THR-382; ALA-385; ILE-386; VAL-387 AND LYS-395. Strain: CN1, CN13, CN14, CN18, CN21, CN28, CN29, CN3, CN43, CN45, CN46, CN51, LA108, LA120, LA128, LA13, LA15, LA25, LA3, LA32, LA34, LA35, LA4, LA6, LA9, MA21, MA24, MA43, MA74, NC100b, NC10b, NC16a, NC1b, NC37a, NC84a, NC88a, NC89a and NC97a.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A spatial map of olfactory receptor expression in the Drosophila antenna." Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R. Cell 96:725-736(1999) [PubMed: 10089887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-397, TISSUE SPECIFICITY. Strain: Oregon-R. Tissue: Antenna.
[6]	"A novel family of divergent seven-transmembrane proteins: candidate odorant receptors in Drosophila." Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R. Neuron 22:327-338(1999) [PubMed: 10069338] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY. Tissue: Antenna.
[7]	"Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo." Benton R., Sachse S., Michnick S.W., Vosshall L.B. PLoS Biol. 4:E20-E20(2006) [PubMed: 16402857] [Abstract] Cited for: FUNCTION, INTERACTION WITH ORCO, TISSUE SPECIFICITY.
[8]	"Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels." Wicher D., Schaefer R., Bauernfeind R., Stensmyr M.C., Heller R., Heinemann S.H., Hansson B.S. Nature 452:1007-1011(2008) [PubMed: 18408711] [Abstract] Cited for: FUNCTION, INTERACTION WITH ORCO.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM212142 Genomic DNA. Translation: CAR79041.1. FM212143 Genomic DNA. Translation: CAR79043.1. FM212144 Genomic DNA. Translation: CAR79045.1. FM212145 Genomic DNA. Translation: CAR79047.1. FM212146 Genomic DNA. Translation: CAR79049.1. FM212147 Genomic DNA. Translation: CAR79051.1. FM212148 Genomic DNA. Translation: CAR79053.1. FM212149 Genomic DNA. Translation: CAR79055.1. FM212150 Genomic DNA. Translation: CAR79057.1. FM212151 Genomic DNA. Translation: CAR79059.1. FM212152 Genomic DNA. Translation: CAR79061.1. FM212153 Genomic DNA. Translation: CAR79063.1. FM212154 Genomic DNA. Translation: CAR79065.1. FM212155 Genomic DNA. Translation: CAR79067.1. FM212156 Genomic DNA. Translation: CAR79069.1. FM212157 Genomic DNA. Translation: CAR79071.1. FM212158 Genomic DNA. Translation: CAR79073.1. FM212159 Genomic DNA. Translation: CAR79075.1. FM212160 Genomic DNA. Translation: CAR79077.1. FM212161 Genomic DNA. Translation: CAR79079.1. FM212162 Genomic DNA. Translation: CAR79081.1. FM212163 Genomic DNA. Translation: CAR79083.1. FM212164 Genomic DNA. Translation: CAR79085.1. FM212165 Genomic DNA. Translation: CAR79087.1. FM212166 Genomic DNA. Translation: CAR79089.1. FM212167 Genomic DNA. Translation: CAR79091.1. FM212168 Genomic DNA. Translation: CAR79093.1. FM212169 Genomic DNA. Translation: CAR79095.1. FM212170 Genomic DNA. Translation: CAR79097.1. FM212171 Genomic DNA. Translation: CAR79099.1. FM212172 Genomic DNA. Translation: CAR79101.1. FM212173 Genomic DNA. Translation: CAR79103.1. FM212174 Genomic DNA. Translation: CAR79105.1. FM212175 Genomic DNA. Translation: CAR79107.1. FM212176 Genomic DNA. Translation: CAR79109.1. FM212177 Genomic DNA. Translation: CAR79111.1. FM212178 Genomic DNA. Translation: CAR79113.1. FM212204 Genomic DNA. Translation: CAR79364.1. AE014134 Genomic DNA. Translation: AAF51364.1. AF127923 mRNA. Translation: AAD26358.2.
RefSeq	NP_523453.1. NM_078729.2.
UniGene	Dm.19764.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-20484N.
IntAct	P81909. 2 interactions.
MINT	MINT-874905.
STRING	P81909.
Protein family/group databases
TCDB	1.A.69.1.1. heteromeric odorant receptor channel (HORC) family.
Proteomic databases
PRIDE	P81909.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0077873; FBpp0077541; FBgn0026398.
GeneID	33335.
KEGG	dme:Dmel_CG12193.
NMPDR	fig|7227.3.peg.255.
Organism-specific databases
CTD	33335.
FlyBase	FBgn0026398. Or22a.
Phylogenomic databases
GeneTree	EMGT00050000009319.
InParanoid	P81909.
OMA	IYFLILR.
OrthoDB	EOG4V6WXZ.
PhylomeDB	P81909.
Gene expression databases
Bgee	P81909.
GermOnline	CG12193. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
KO	K08471.
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	783083.

Entry information

Entry name

OR22A_DROME

Accession

Primary (citable) accession number: P81909
Secondary accession number(s): B7VC05 , B7VC07, B7VC09, B7VC11, B7VC21, B7VC27, B7VC29, B7VC75, B7VC77, B7VCA5, Q9U6X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1999
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project