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P81908

- CHLE_HORSE

UniProt

P81908 - CHLE_HORSE

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Protein

Cholinesterase

Gene

BCHE

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters (By similarity).By similarity

Catalytic activityi

An acylcholine + H2O = choline + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei325 – 3251Charge relay systemBy similarity
Active sitei438 – 4381Charge relay systemBy similarity

GO - Molecular functioni

  1. acetylcholinesterase activity Source: UniProtKB
  2. cholinesterase activity Source: UniProtKB

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

MEROPSiS09.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinesterase (EC:3.1.1.8)
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
EQ-BCHE
Pseudocholinesterase
Gene namesi
Name:BCHE
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574CholinesterasePRO_0000070285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)
Disulfide bondi65 ↔ 92By similarity
Glycosylationi106 – 1061N-linked (GlcNAc...)
Modified residuei198 – 1981PhosphoserineBy similarity
Glycosylationi241 – 2411N-linked (GlcNAc...)
Disulfide bondi252 ↔ 263By similarity
Glycosylationi256 – 2561N-linked (GlcNAc...)
Glycosylationi341 – 3411N-linked (GlcNAc...)
Disulfide bondi400 ↔ 519By similarity
Glycosylationi455 – 4551N-linked (GlcNAc...)
Glycosylationi481 – 4811N-linked (GlcNAc...)
Glycosylationi486 – 4861N-linked (GlcNAc...)
Disulfide bondi571 – 571InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Detected in blood plasma (at protein level). Present in most cells except erythrocytes.1 Publication

Interactioni

Subunit structurei

Homotetramer; disulfide-linked. Dimer of dimers (By similarity).By similarity

Protein-protein interaction databases

STRINGi9796.ENSECAP00000000166.

Structurei

3D structure databases

ProteinModelPortaliP81908.
SMRiP81908. Positions 4-534, 536-567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni116 – 1172Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2272.
HOVERGENiHBG008839.
InParanoidiP81908.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P81908-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
EEDIIITTKN GKVRGMNLPV LGGTVTAFLG IPYAQPPLGR LRFKKPQSLT
60 70 80 90 100
KWSNIWNATK YANSCYQNTD QSFPGFLGSE MWNPNTELSE DCLYLNVWIP
110 120 130 140 150
APKPKNATVM IWIYGGGFQT GTSSLPVYDG KFLARVERVI VVSMNYRVGA
160 170 180 190 200
LGFLALSENP EAPGNMGLFD QQLALQWVQK NIAAFGGNPR SVTLFGESAG
210 220 230 240 250
AASVSLHLLS PRSQPLFTRA ILQSGSSNAP WAVTSLYEAR NRTLTLAKRM
260 270 280 290 300
GCSRDNETEM IKCLRDKDPQ EILLNEVFVV PYDTLLSVNF GPTVDGDFLT
310 320 330 340 350
DMPDTLLQLG QFKRTQILVG VNKDEGTAFL VYGAPGFSKD NNSIITRKEF
360 370 380 390 400
QEGLKIFFPR VSEFGRESIL FHYMDWLDDQ RAENYREALD DVVGDYNIIC
410 420 430 440 450
PALEFTRKFS ELGNDAFFYY FEHRSTKLPW PEWMGVMHGY EIEFVFGLPL
460 470 480 490 500
ERRVNYTRAE EILSRSIMKR WANFAKYGNP NGTQNNSTRW PVFKSTEQKY
510 520 530 540 550
LTLNTESPKV YTKLRAQQCR FWTLFFPKVL ELTGNIDEAE REWKAGFHRW
560 570
NNYMMDWKNQ FNDYTSKKES CSDF
Length:574
Mass (Da):65,642
Last modified:May 1, 2000 - v1
Checksum:i07755EE9FB9CB33E
GO

Sequence databases

UniGeneiEca.13015.

Cross-referencesi

Sequence databases

UniGenei Eca.13015.

3D structure databases

ProteinModelPortali P81908.
SMRi P81908. Positions 4-534, 536-567.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9796.ENSECAP00000000166.

Chemistry

BindingDBi P81908.
ChEMBLi CHEMBL5763.

Protein family/group databases

MEROPSi S09.980.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG2272.
HOVERGENi HBG008839.
InParanoidi P81908.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view ]
Pfami PF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view ]
PRINTSi PR00878. CHOLNESTRASE.
ProDomi PD415333. AChE_tetra. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amino acid sequence of horse serum butyrycholinesterase."
    Moorad D.R., Luo C., Garcia G.E., Doctor B.P.
    (In) Doctor B.P., Taylor P., Quinn D.M., Rotundo R.L., Gentry M.K. (eds.); Structure and function of cholinesterases and related proteins, pp.145-146, Plenum Press, New York and London (1998)
    Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Plasma.

Entry informationi

Entry nameiCHLE_HORSE
AccessioniPrimary (citable) accession number: P81908
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3