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P81908 (CHLE_HORSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholinesterase

EC=3.1.1.8
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
EQ-BCHE
Pseudocholinesterase
Gene names
Name:BCHE
OrganismEquus caballus (Horse) [Reference proteome]
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters By similarity.

Catalytic activity

An acylcholine + H2O = choline + a carboxylate.

Subunit structure

Homotetramer; disulfide-linked. Dimer of dimers By similarity.

Subcellular location

Secreted Ref.1.

Tissue specificity

Detected in blood plasma (at protein level). Present in most cells except erythrocytes. Ref.1

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 574574Cholinesterase
PRO_0000070285

Regions

Region116 – 1172Substrate binding By similarity

Sites

Active site1981Acyl-ester intermediate By similarity
Active site3251Charge relay system By similarity
Active site4381Charge relay system By similarity

Amino acid modifications

Modified residue1981Phosphoserine By similarity
Glycosylation571N-linked (GlcNAc...)
Glycosylation1061N-linked (GlcNAc...)
Glycosylation2411N-linked (GlcNAc...)
Glycosylation2561N-linked (GlcNAc...)
Glycosylation3411N-linked (GlcNAc...)
Glycosylation4551N-linked (GlcNAc...)
Glycosylation4811N-linked (GlcNAc...)
Glycosylation4861N-linked (GlcNAc...)
Disulfide bond65 ↔ 92 By similarity
Disulfide bond252 ↔ 263 By similarity
Disulfide bond400 ↔ 519 By similarity
Disulfide bond571Interchain By similarity

Sequences

Sequence LengthMass (Da)Tools
P81908 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 07755EE9FB9CB33E

FASTA57465,642
        10         20         30         40         50         60 
EEDIIITTKN GKVRGMNLPV LGGTVTAFLG IPYAQPPLGR LRFKKPQSLT KWSNIWNATK 

        70         80         90        100        110        120 
YANSCYQNTD QSFPGFLGSE MWNPNTELSE DCLYLNVWIP APKPKNATVM IWIYGGGFQT 

       130        140        150        160        170        180 
GTSSLPVYDG KFLARVERVI VVSMNYRVGA LGFLALSENP EAPGNMGLFD QQLALQWVQK 

       190        200        210        220        230        240 
NIAAFGGNPR SVTLFGESAG AASVSLHLLS PRSQPLFTRA ILQSGSSNAP WAVTSLYEAR 

       250        260        270        280        290        300 
NRTLTLAKRM GCSRDNETEM IKCLRDKDPQ EILLNEVFVV PYDTLLSVNF GPTVDGDFLT 

       310        320        330        340        350        360 
DMPDTLLQLG QFKRTQILVG VNKDEGTAFL VYGAPGFSKD NNSIITRKEF QEGLKIFFPR 

       370        380        390        400        410        420 
VSEFGRESIL FHYMDWLDDQ RAENYREALD DVVGDYNIIC PALEFTRKFS ELGNDAFFYY 

       430        440        450        460        470        480 
FEHRSTKLPW PEWMGVMHGY EIEFVFGLPL ERRVNYTRAE EILSRSIMKR WANFAKYGNP 

       490        500        510        520        530        540 
NGTQNNSTRW PVFKSTEQKY LTLNTESPKV YTKLRAQQCR FWTLFFPKVL ELTGNIDEAE 

       550        560        570 
REWKAGFHRW NNYMMDWKNQ FNDYTSKKES CSDF 

« Hide

References

[1]"Amino acid sequence of horse serum butyrycholinesterase."
Moorad D.R., Luo C., Garcia G.E., Doctor B.P.
(In) Doctor B.P., Taylor P., Quinn D.M., Rotundo R.L., Gentry M.K. (eds.); Structure and function of cholinesterases and related proteins, pp.145-146, Plenum Press, New York and London (1998)
Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Plasma.

Cross-references

Sequence databases

UniGeneEca.13015.

3D structure databases

ProteinModelPortalP81908.
SMRP81908. Positions 4-534, 536-567.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9796.ENSECAP00000000166.

Chemistry

BindingDBP81908.
ChEMBLCHEMBL5763.

Protein family/group databases

MEROPSS09.980.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG2272.
HOVERGENHBG008839.
InParanoidP81908.

Family and domain databases

InterProIPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR00878. CHOLNESTRASE.
ProDomPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHLE_HORSE
AccessionPrimary (citable) accession number: P81908
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families