Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P81908

- CHLE_HORSE

UniProt

P81908 - CHLE_HORSE

Protein

Cholinesterase

Gene

BCHE

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters By similarity.By similarity

    Catalytic activityi

    An acylcholine + H2O = choline + a carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei198 – 1981Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei325 – 3251Charge relay systemBy similarity
    Active sitei438 – 4381Charge relay systemBy similarity

    GO - Molecular functioni

    1. acetylcholinesterase activity Source: UniProtKB
    2. cholinesterase activity Source: UniProtKB

    GO - Biological processi

    1. metabolic process Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Protein family/group databases

    MEROPSiS09.980.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholinesterase (EC:3.1.1.8)
    Alternative name(s):
    Acylcholine acylhydrolase
    Butyrylcholine esterase
    Choline esterase II
    EQ-BCHE
    Pseudocholinesterase
    Gene namesi
    Name:BCHE
    OrganismiEquus caballus (Horse)
    Taxonomic identifieri9796 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
    ProteomesiUP000002281: Unplaced

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574CholinesterasePRO_0000070285Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)
    Disulfide bondi65 ↔ 92By similarity
    Glycosylationi106 – 1061N-linked (GlcNAc...)
    Modified residuei198 – 1981PhosphoserineBy similarity
    Glycosylationi241 – 2411N-linked (GlcNAc...)
    Disulfide bondi252 ↔ 263By similarity
    Glycosylationi256 – 2561N-linked (GlcNAc...)
    Glycosylationi341 – 3411N-linked (GlcNAc...)
    Disulfide bondi400 ↔ 519By similarity
    Glycosylationi455 – 4551N-linked (GlcNAc...)
    Glycosylationi481 – 4811N-linked (GlcNAc...)
    Glycosylationi486 – 4861N-linked (GlcNAc...)
    Disulfide bondi571 – 571InterchainBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Expressioni

    Tissue specificityi

    Detected in blood plasma (at protein level). Present in most cells except erythrocytes.1 Publication

    Interactioni

    Subunit structurei

    Homotetramer; disulfide-linked. Dimer of dimers By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9796.ENSECAP00000000166.

    Structurei

    3D structure databases

    ProteinModelPortaliP81908.
    SMRiP81908. Positions 4-534, 536-567.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni116 – 1172Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2272.
    HOVERGENiHBG008839.
    InParanoidiP81908.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view]
    PfamiPF08674. AChE_tetra. 1 hit.
    PF00135. COesterase. 1 hit.
    [Graphical view]
    PRINTSiPR00878. CHOLNESTRASE.
    ProDomiPD415333. AChE_tetra. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P81908-1 [UniParc]FASTAAdd to Basket

    « Hide

    EEDIIITTKN GKVRGMNLPV LGGTVTAFLG IPYAQPPLGR LRFKKPQSLT    50
    KWSNIWNATK YANSCYQNTD QSFPGFLGSE MWNPNTELSE DCLYLNVWIP 100
    APKPKNATVM IWIYGGGFQT GTSSLPVYDG KFLARVERVI VVSMNYRVGA 150
    LGFLALSENP EAPGNMGLFD QQLALQWVQK NIAAFGGNPR SVTLFGESAG 200
    AASVSLHLLS PRSQPLFTRA ILQSGSSNAP WAVTSLYEAR NRTLTLAKRM 250
    GCSRDNETEM IKCLRDKDPQ EILLNEVFVV PYDTLLSVNF GPTVDGDFLT 300
    DMPDTLLQLG QFKRTQILVG VNKDEGTAFL VYGAPGFSKD NNSIITRKEF 350
    QEGLKIFFPR VSEFGRESIL FHYMDWLDDQ RAENYREALD DVVGDYNIIC 400
    PALEFTRKFS ELGNDAFFYY FEHRSTKLPW PEWMGVMHGY EIEFVFGLPL 450
    ERRVNYTRAE EILSRSIMKR WANFAKYGNP NGTQNNSTRW PVFKSTEQKY 500
    LTLNTESPKV YTKLRAQQCR FWTLFFPKVL ELTGNIDEAE REWKAGFHRW 550
    NNYMMDWKNQ FNDYTSKKES CSDF 574
    Length:574
    Mass (Da):65,642
    Last modified:May 1, 2000 - v1
    Checksum:i07755EE9FB9CB33E
    GO

    Sequence databases

    UniGeneiEca.13015.

    Cross-referencesi

    Sequence databases

    UniGenei Eca.13015.

    3D structure databases

    ProteinModelPortali P81908.
    SMRi P81908. Positions 4-534, 536-567.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9796.ENSECAP00000000166.

    Chemistry

    BindingDBi P81908.
    ChEMBLi CHEMBL5763.

    Protein family/group databases

    MEROPSi S09.980.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG2272.
    HOVERGENi HBG008839.
    InParanoidi P81908.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view ]
    Pfami PF08674. AChE_tetra. 1 hit.
    PF00135. COesterase. 1 hit.
    [Graphical view ]
    PRINTSi PR00878. CHOLNESTRASE.
    ProDomi PD415333. AChE_tetra. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of horse serum butyrycholinesterase."
      Moorad D.R., Luo C., Garcia G.E., Doctor B.P.
      (In) Doctor B.P., Taylor P., Quinn D.M., Rotundo R.L., Gentry M.K. (eds.); Structure and function of cholinesterases and related proteins, pp.145-146, Plenum Press, New York and London (1998)
      Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Plasma.

    Entry informationi

    Entry nameiCHLE_HORSE
    AccessioniPrimary (citable) accession number: P81908
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 6, 2002
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3