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P81800 (PPB1_PREIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alkaline phosphatase

Short name=ALPase
EC=3.1.3.1
Alternative name(s):
PiALP
OrganismPrevotella intermedia
Taxonomic identifier28131 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesPrevotellaceaePrevotella

Protein attributes

Sequence length20 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts against tyrosine-phosphatases.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Enzyme regulation

Completely inhibited by thiol-reducing agents, such as DTT and 2-mercaptoethanol. Activity was also inhibited by sodium orthovanadate, sodium molybdate, N-ethylmaleimide, EDTA and zinc ion, but was not inhibited by okadaic acid.

Subunit structure

Homodimer; may be disulfide-linked.

Post-translational modification

The N-terminus is blocked.

Ontologies

Keywords
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from direct assay Ref.1. Source: GOC

metabolic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionalkaline phosphatase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›20›20Alkaline phosphatase
PRO_0000058533

Experimental info

Non-terminal residue11
Non-terminal residue201

Sequences

Sequence LengthMass (Da)Tools
P81800 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: A8D8A9276AE0ED83

FASTA202,053
        10         20 
TDMLAVSVSS TDAIGHKYGT 

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References

[1]"Purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium Prevotella intermedia."
Ansai T., Awano S., Chen X., Fuchi T., Arimoto T., Akifusa S., Takehara T.
FEBS Lett. 428:157-160(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, CHARACTERIZATION.
Strain: ATCC 25611 / DSM 20706 / JCM 12248 / NCTC 13070 / VPI 4197.

Cross-references

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry namePPB1_PREIN
AccessionPrimary (citable) accession number: P81800
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program