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Reviewed, UniProtKB/Swiss-Prot P81800 (PPB1_PREIN)

Last modified March 3, 2009. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information

Names and origin

Protein namesRecommended name:
    Alkaline phosphatase
      Short name=ALPase
    EC=3.1.3.1
Alternative name(s):
    PiALP
OrganismPrevotella intermedia
Taxonomic identifier28131 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesPrevotellaceaePrevotella

Protein attributes

Sequence length20 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts against tyrosine-phosphatases.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Enzyme regulation

Completely inhibited by thiol-reducing agents, such as DTT and 2-mercaptoethanol. Activity was also inhibited by sodium orthovanadate, sodium molybdate, N-ethylmaleimide, EDTA and zinc ion, but was not inhibited by okadaic acid.

Subunit structure

Homodimer; may be disulfide-linked.

Post-translational modification

The N-terminus is blocked.

Ontologies

Keywords
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process Ref.1

Inferred from direct assay. Source: UniProtKB

   Molecular functionalkaline phosphatase activity Ref.1

Inferred from direct assay. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›20›20Alkaline phosphatase
PRO_0000058533

Experimental info

Non-terminal residue11
Non-terminal residue201

Sequences

Sequence LengthMass (Da)Tools
P81800-1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: A8D8A9276AE0ED83

FASTA202,053
        10         20 
TDMLAVSVSS TDAIGHKYGT 

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References

[1]"Purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium Prevotella intermedia."
Ansai T., Awano S., Chen X., Fuchi T., Arimoto T., Akifusa S., Takehara T.
FEBS Lett. 428:157-160(1998) [PubMed: 9654126] [Abstract]
Cited for: PROTEIN SEQUENCE, CHARACTERIZATION.
Strain: ATCC 25611 / DSM 20706 / JCM 12248 / NCTC 13070 / VPI 4197.

Cross-references

3D structure databases

ModBaseSearch...

Enzyme and pathway databases

BRENDA3.1.3.1. 74268.

Family and domain databases

ProtoNetSearch...

Entry information

Entry namePPB1_PREIN
AccessionPrimary (citable) accession number: P81800
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2001
Last modified: March 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information