Reviewed, UniProtKB/Swiss-Prot P81800 (PPB1_PREIN)

Names and origin

Protein names	Recommended name:     Alkaline phosphatase       Short name=ALPase     EC=3.1.3.1 Alternative name(s):     PiALP
Organism	Prevotella intermedia
Taxonomic identifier	28131 [NCBI]
Taxonomic lineage	Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Prevotellaceae › Prevotella

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts against tyrosine-phosphatases.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Enzyme regulation	Completely inhibited by thiol-reducing agents, such as DTT and 2-mercaptoethanol. Activity was also inhibited by sodium orthovanadate, sodium molybdate, N-ethylmaleimide, EDTA and zinc ion, but was not inhibited by okadaic acid.
Subunit structure	Homodimer; may be disulfide-linked.
Post-translational modification	The N-terminus is blocked.

Ontologies

Keywords
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	alkaline phosphatase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›20	›20	Alkaline phosphatase		PRO_0000058533
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	P81800-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: A8D8A9276AE0ED83	FASTA	20	2,053
	10 20 TDMLAVSVSS TDAIGHKYGT

References

[1]

"Purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium Prevotella intermedia." Ansai T., Awano S., Chen X., Fuchi T., Arimoto T., Akifusa S., Takehara T. FEBS Lett. 428:157-160(1998) [PubMed: 9654126] [Abstract] Cited for: PROTEIN SEQUENCE, CHARACTERIZATION. Strain: ATCC 25611 / DSM 20706 / JCM 12248 / NCTC 13070 / VPI 4197.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.1. 74268.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PPB1_PREIN

Accession

Primary (citable) accession number: P81800

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2001
Last modified:	March 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)