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P81799

- NAGK_RAT

UniProt

P81799 - NAGK_RAT

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Protein

N-acetyl-D-glucosamine kinase

Gene

Nagk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.1 Publication

Catalytic activityi

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.

Enzyme regulationi

Inhibited by the cysteine modifiers iodoacetamide, N-ethylmaleimide and 5,5'-dithiobis(2-nitrobenzoic acid).1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateBy similarity
Binding sitei107 – 1071SubstrateBy similarity
Binding sitei152 – 1521SubstrateBy similarity
Binding sitei271 – 2711ATPBy similarity
Binding sitei275 – 2751ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 138ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. N-acetylglucosamine kinase activity Source: UniProtKB
  3. N-acylmannosamine kinase activity Source: Ensembl

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. N-acetylglucosamine metabolic process Source: Ensembl
  3. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP81799.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyl-D-glucosamine kinase (EC:2.7.1.59)
Short name:
N-acetylglucosamine kinase
Alternative name(s):
GlcNAc kinase
Gene namesi
Name:Nagk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi1305057. Nagk.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 343342N-acetyl-D-glucosamine kinasePRO_0000096698Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei76 – 761PhosphoserineBy similarity
Modified residuei205 – 2051PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP81799.

PTM databases

PhosphoSiteiP81799.

Expressioni

Gene expression databases

GenevestigatoriP81799.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP81799. 1 interaction.
STRINGi10116.ENSRNOP00000039247.

Structurei

3D structure databases

ProteinModelPortaliP81799.
SMRiP81799. Positions 2-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1302Substrate bindingBy similarity
Regioni145 – 1473Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2971.
GeneTreeiENSGT00510000047418.
HOGENOMiHOG000007248.
HOVERGENiHBG052570.
InParanoidiP81799.
KOiK00884.
OMAiAFYSYTF.
OrthoDBiEOG7V1FR7.
PhylomeDBiP81799.
TreeFamiTF314158.

Family and domain databases

InterProiIPR002731. ATPase_BadF.
[Graphical view]
PfamiPF01869. BcrAD_BadFG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81799-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAALYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTGTCVERIN
60 70 80 90 100
EMVDRAKRKA GVDPLVPLRS LGLSLSGGEQ EDAVRLLMEE LRDRFPYLSE
110 120 130 140 150
SYFITTDAAG SIATATPDGG IVLISGTGSN CRLINPDGSE SGCGGWGHMM
160 170 180 190 200
GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD IGHVKQAMFN YFQVPDRLGI
210 220 230 240 250
LTHLYRDFDK SKFAGFCQKI AEGAQQGDPL SRFIFRKAGE MLGRHVVAVL
260 270 280 290 300
PEIDPVLFQG ELGLPILCVG SVWKSWELLK EGFLLALTQG REQQAQNSFS
310 320 330 340
SFTLMKLRHS SALGGASLGA RHIGHHLPMD YSVNAIAFYS YTF
Length:343
Mass (Da):37,196
Last modified:January 23, 2007 - v4
Checksum:i43886E3262D08A0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti207 – 2071D → T AA sequence (PubMed:9523722)Curated
Sequence conflicti226 – 2261Q → E AA sequence (PubMed:9523722)Curated
Sequence conflicti309 – 3091H → I AA sequence (PubMed:9523722)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC107647 mRNA. Translation: AAI07648.1.
RefSeqiNP_001032857.1. NM_001037768.1.
UniGeneiRn.105985.

Genome annotation databases

EnsembliENSRNOT00000042875; ENSRNOP00000039247; ENSRNOG00000013911.
GeneIDi297393.
KEGGirno:297393.
UCSCiRGD:1305057. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC107647 mRNA. Translation: AAI07648.1 .
RefSeqi NP_001032857.1. NM_001037768.1.
UniGenei Rn.105985.

3D structure databases

ProteinModelPortali P81799.
SMRi P81799. Positions 2-343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P81799. 1 interaction.
STRINGi 10116.ENSRNOP00000039247.

PTM databases

PhosphoSitei P81799.

Proteomic databases

PRIDEi P81799.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000042875 ; ENSRNOP00000039247 ; ENSRNOG00000013911 .
GeneIDi 297393.
KEGGi rno:297393.
UCSCi RGD:1305057. rat.

Organism-specific databases

CTDi 55577.
RGDi 1305057. Nagk.

Phylogenomic databases

eggNOGi COG2971.
GeneTreei ENSGT00510000047418.
HOGENOMi HOG000007248.
HOVERGENi HBG052570.
InParanoidi P81799.
KOi K00884.
OMAi AFYSYTF.
OrthoDBi EOG7V1FR7.
PhylomeDBi P81799.
TreeFami TF314158.

Enzyme and pathway databases

UniPathwayi UPA00629 .
SABIO-RK P81799.

Miscellaneous databases

NextBioi 642192.
PROi P81799.

Gene expression databases

Genevestigatori P81799.

Family and domain databases

InterProi IPR002731. ATPase_BadF.
[Graphical view ]
Pfami PF01869. BcrAD_BadFG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  2. "Purification and characterization of N-acetylglucosamine kinase from rat liver. Comparison with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase."
    Hinderlich S., Noehring S., Weise C., Franke P., Staesche R., Reutter W.
    Eur. J. Biochem. 252:133-139(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-13; 69-92; 166-178; 198-207; 221-228; 233-235 AND 308-321, ACETYLATION AT ALA-2, ENZYME REGULATION, SUBUNIT, FUNCTION.
    Strain: Wistar.
    Tissue: Liver.

Entry informationi

Entry nameiNAGK_RAT
AccessioniPrimary (citable) accession number: P81799
Secondary accession number(s): Q32Q91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 72 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3