Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P81799

- NAGK_RAT

UniProt

P81799 - NAGK_RAT

Protein

N-acetyl-D-glucosamine kinase

Gene

Nagk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.1 Publication

    Catalytic activityi

    ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.

    Enzyme regulationi

    Inhibited by the cysteine modifiers iodoacetamide, N-ethylmaleimide and 5,5'-dithiobis(2-nitrobenzoic acid).1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361SubstrateBy similarity
    Binding sitei107 – 1071SubstrateBy similarity
    Binding sitei152 – 1521SubstrateBy similarity
    Binding sitei271 – 2711ATPBy similarity
    Binding sitei275 – 2751ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi6 – 138ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. N-acetylglucosamine kinase activity Source: UniProtKB
    3. N-acylmannosamine kinase activity Source: Ensembl

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. N-acetylglucosamine metabolic process Source: Ensembl
    3. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKP81799.
    UniPathwayiUPA00629.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetyl-D-glucosamine kinase (EC:2.7.1.59)
    Short name:
    N-acetylglucosamine kinase
    Alternative name(s):
    GlcNAc kinase
    Gene namesi
    Name:Nagk
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi1305057. Nagk.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 343342N-acetyl-D-glucosamine kinasePRO_0000096698Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei76 – 761PhosphoserineBy similarity
    Modified residuei205 – 2051PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP81799.

    PTM databases

    PhosphoSiteiP81799.

    Expressioni

    Gene expression databases

    GenevestigatoriP81799.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiP81799. 1 interaction.
    STRINGi10116.ENSRNOP00000039247.

    Structurei

    3D structure databases

    ProteinModelPortaliP81799.
    SMRiP81799. Positions 2-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 1302Substrate bindingBy similarity
    Regioni145 – 1473Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2971.
    GeneTreeiENSGT00510000047418.
    HOGENOMiHOG000007248.
    HOVERGENiHBG052570.
    KOiK00884.
    OMAiAFYSYTF.
    OrthoDBiEOG7V1FR7.
    PhylomeDBiP81799.
    TreeFamiTF314158.

    Family and domain databases

    InterProiIPR002731. ATPase_BadF.
    [Graphical view]
    PfamiPF01869. BcrAD_BadFG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P81799-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAALYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTGTCVERIN    50
    EMVDRAKRKA GVDPLVPLRS LGLSLSGGEQ EDAVRLLMEE LRDRFPYLSE 100
    SYFITTDAAG SIATATPDGG IVLISGTGSN CRLINPDGSE SGCGGWGHMM 150
    GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD IGHVKQAMFN YFQVPDRLGI 200
    LTHLYRDFDK SKFAGFCQKI AEGAQQGDPL SRFIFRKAGE MLGRHVVAVL 250
    PEIDPVLFQG ELGLPILCVG SVWKSWELLK EGFLLALTQG REQQAQNSFS 300
    SFTLMKLRHS SALGGASLGA RHIGHHLPMD YSVNAIAFYS YTF 343
    Length:343
    Mass (Da):37,196
    Last modified:January 23, 2007 - v4
    Checksum:i43886E3262D08A0F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti207 – 2071D → T AA sequence (PubMed:9523722)Curated
    Sequence conflicti226 – 2261Q → E AA sequence (PubMed:9523722)Curated
    Sequence conflicti309 – 3091H → I AA sequence (PubMed:9523722)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC107647 mRNA. Translation: AAI07648.1.
    RefSeqiNP_001032857.1. NM_001037768.1.
    UniGeneiRn.105985.

    Genome annotation databases

    EnsembliENSRNOT00000042875; ENSRNOP00000039247; ENSRNOG00000013911.
    GeneIDi297393.
    KEGGirno:297393.
    UCSCiRGD:1305057. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC107647 mRNA. Translation: AAI07648.1 .
    RefSeqi NP_001032857.1. NM_001037768.1.
    UniGenei Rn.105985.

    3D structure databases

    ProteinModelPortali P81799.
    SMRi P81799. Positions 2-343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P81799. 1 interaction.
    STRINGi 10116.ENSRNOP00000039247.

    PTM databases

    PhosphoSitei P81799.

    Proteomic databases

    PRIDEi P81799.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000042875 ; ENSRNOP00000039247 ; ENSRNOG00000013911 .
    GeneIDi 297393.
    KEGGi rno:297393.
    UCSCi RGD:1305057. rat.

    Organism-specific databases

    CTDi 55577.
    RGDi 1305057. Nagk.

    Phylogenomic databases

    eggNOGi COG2971.
    GeneTreei ENSGT00510000047418.
    HOGENOMi HOG000007248.
    HOVERGENi HBG052570.
    KOi K00884.
    OMAi AFYSYTF.
    OrthoDBi EOG7V1FR7.
    PhylomeDBi P81799.
    TreeFami TF314158.

    Enzyme and pathway databases

    UniPathwayi UPA00629 .
    SABIO-RK P81799.

    Miscellaneous databases

    NextBioi 642192.
    PROi P81799.

    Gene expression databases

    Genevestigatori P81799.

    Family and domain databases

    InterProi IPR002731. ATPase_BadF.
    [Graphical view ]
    Pfami PF01869. BcrAD_BadFG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    2. "Purification and characterization of N-acetylglucosamine kinase from rat liver. Comparison with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase."
      Hinderlich S., Noehring S., Weise C., Franke P., Staesche R., Reutter W.
      Eur. J. Biochem. 252:133-139(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-13; 69-92; 166-178; 198-207; 221-228; 233-235 AND 308-321, ACETYLATION AT ALA-2, ENZYME REGULATION, SUBUNIT, FUNCTION.
      Strain: Wistar.
      Tissue: Liver.

    Entry informationi

    Entry nameiNAGK_RAT
    AccessioniPrimary (citable) accession number: P81799
    Secondary accession number(s): Q32Q91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 71 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3