P81799 (NAGK_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-D-glucosamine kinase
Short name=N-acetylglucosamine kinase
EC=2.7.1.59

Alternative name(s):
GlcNAc kinase

Gene names

Name:

Nagk

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity. Ref.2
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.
Enzyme regulation	Inhibited by the cysteine modifiers iodoacetamide, N-ethylmaleimide and 5,5'-dithiobis(2-nitrobenzoic acid). Ref.2
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation.
Subunit structure	Homodimer. Ref.2
Post-translational modification	The N-terminal is blocked.
Sequence similarities	Belongs to the eukaryotic-type N-acetylglucosamine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: Compara N-acetylneuraminate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acetylglucosamine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB N-acylmannosamine kinase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 343	342	N-acetyl-D-glucosamine kinase		PRO_0000096698
Regions
	Nucleotide binding	6 – 13	8	ATP By similarity
	Region	129 – 130	2	Substrate binding By similarity
	Region	145 – 147	3	Substrate binding By similarity
Sites
	Binding site	36	1	Substrate By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	271	1	ATP By similarity
	Binding site	275	1	ATP By similarity
Amino acid modifications
	Modified residue	76	1	Phosphoserine By similarity
	Modified residue	205	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	207	1	D → T AA sequence Ref.2
	Sequence conflict	226	1	Q → E AA sequence Ref.2
	Sequence conflict	309	1	H → I AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P81799 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 43886E3262D08A0F	FASTA	343	37,196
	10 20 30 40 50 60 MAALYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTGTCVERIN EMVDRAKRKA 70 80 90 100 110 120 GVDPLVPLRS LGLSLSGGEQ EDAVRLLMEE LRDRFPYLSE SYFITTDAAG SIATATPDGG 130 140 150 160 170 180 IVLISGTGSN CRLINPDGSE SGCGGWGHMM GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD 190 200 210 220 230 240 IGHVKQAMFN YFQVPDRLGI LTHLYRDFDK SKFAGFCQKI AEGAQQGDPL SRFIFRKAGE 250 260 270 280 290 300 MLGRHVVAVL PEIDPVLFQG ELGLPILCVG SVWKSWELLK EGFLLALTQG REQQAQNSFS 310 320 330 340 SFTLMKLRHS SALGGASLGA RHIGHHLPMD YSVNAIAFYS YTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[2]	"Purification and characterization of N-acetylglucosamine kinase from rat liver. Comparison with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase." Hinderlich S., Noehring S., Weise C., Franke P., Staesche R., Reutter W. Eur. J. Biochem. 252:133-139(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-13; 69-92; 166-178; 198-207; 221-228; 233-235 AND 308-321, ENZYME REGULATION, SUBUNIT, FUNCTION. Strain: Wistar. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC107647 mRNA. Translation: AAI07648.1.
IPI	IPI00464455.
RefSeq	NP_001032857.1. NM_001037768.1.
UniGene	Rn.105985.
3D structure databases
ProteinModelPortal	P81799.
SMR	P81799. Positions 2-343.
ModBase	Search...
Protein-protein interaction databases
IntAct	P81799. 1 interaction.
STRING	10116.ENSRNOP00000039247.
PTM databases
PhosphoSite	P81799.
Proteomic databases
PRIDE	P81799.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000042875; ENSRNOP00000039247; ENSRNOG00000013911.
GeneID	297393.
KEGG	rno:297393.
UCSC	RGD:1305057. rat.
Organism-specific databases
CTD	55577.
RGD	1305057. Nagk.
Phylogenomic databases
eggNOG	COG2971.
GeneTree	ENSGT00510000047418.
HOGENOM	HOG000007248.
HOVERGEN	HBG052570.
KO	K00884.
OrthoDB	EOG4GF3FF.
Enzyme and pathway databases
SABIO-RK	P81799.
UniPathway	UPA00629.
Gene expression databases
Genevestigator	P81799.
Family and domain databases
InterPro	IPR002731. ATPase_BadF. [Graphical view]
Pfam	PF01869. BcrAD_BadFG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	642192.

Entry information

Entry name

NAGK_RAT

Accession

Primary (citable) accession number: P81799
Secondary accession number(s): Q32Q91

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 64 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program