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Protein

N-acetyl-D-glucosamine kinase

Gene

Nagk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.1 Publication

Catalytic activityi

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.

Enzyme regulationi

Inhibited by the cysteine modifiers iodoacetamide, N-ethylmaleimide and 5,5'-dithiobis(2-nitrobenzoic acid).1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateBy similarity
Binding sitei107 – 1071SubstrateBy similarity
Binding sitei152 – 1521SubstrateBy similarity
Binding sitei271 – 2711ATPBy similarity
Binding sitei275 – 2751ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 138ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. N-acetylglucosamine kinase activity Source: UniProtKB
  3. N-acylmannosamine kinase activity Source: Ensembl

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. N-acetylglucosamine metabolic process Source: Ensembl
  3. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP81799.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyl-D-glucosamine kinase (EC:2.7.1.59)
Short name:
N-acetylglucosamine kinase
Alternative name(s):
GlcNAc kinase
Gene namesi
Name:Nagk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi1305057. Nagk.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 343342N-acetyl-D-glucosamine kinasePRO_0000096698Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei76 – 761PhosphoserineBy similarity
Modified residuei205 – 2051PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP81799.

PTM databases

PhosphoSiteiP81799.

Expressioni

Gene expression databases

GenevestigatoriP81799.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP81799. 1 interaction.
STRINGi10116.ENSRNOP00000039247.

Structurei

3D structure databases

ProteinModelPortaliP81799.
SMRiP81799. Positions 2-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1302Substrate bindingBy similarity
Regioni145 – 1473Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2971.
GeneTreeiENSGT00510000047418.
HOGENOMiHOG000007248.
HOVERGENiHBG052570.
InParanoidiP81799.
KOiK00884.
OMAiLLPMDYS.
OrthoDBiEOG7V1FR7.
PhylomeDBiP81799.
TreeFamiTF314158.

Family and domain databases

InterProiIPR002731. ATPase_BadF.
[Graphical view]
PfamiPF01869. BcrAD_BadFG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTGTCVERIN
60 70 80 90 100
EMVDRAKRKA GVDPLVPLRS LGLSLSGGEQ EDAVRLLMEE LRDRFPYLSE
110 120 130 140 150
SYFITTDAAG SIATATPDGG IVLISGTGSN CRLINPDGSE SGCGGWGHMM
160 170 180 190 200
GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD IGHVKQAMFN YFQVPDRLGI
210 220 230 240 250
LTHLYRDFDK SKFAGFCQKI AEGAQQGDPL SRFIFRKAGE MLGRHVVAVL
260 270 280 290 300
PEIDPVLFQG ELGLPILCVG SVWKSWELLK EGFLLALTQG REQQAQNSFS
310 320 330 340
SFTLMKLRHS SALGGASLGA RHIGHHLPMD YSVNAIAFYS YTF
Length:343
Mass (Da):37,196
Last modified:January 23, 2007 - v4
Checksum:i43886E3262D08A0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti207 – 2071D → T AA sequence (PubMed:9523722).Curated
Sequence conflicti226 – 2261Q → E AA sequence (PubMed:9523722).Curated
Sequence conflicti309 – 3091H → I AA sequence (PubMed:9523722).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107647 mRNA. Translation: AAI07648.1.
RefSeqiNP_001032857.1. NM_001037768.1.
UniGeneiRn.105985.

Genome annotation databases

EnsembliENSRNOT00000042875; ENSRNOP00000039247; ENSRNOG00000013911.
GeneIDi297393.
KEGGirno:297393.
UCSCiRGD:1305057. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107647 mRNA. Translation: AAI07648.1.
RefSeqiNP_001032857.1. NM_001037768.1.
UniGeneiRn.105985.

3D structure databases

ProteinModelPortaliP81799.
SMRiP81799. Positions 2-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP81799. 1 interaction.
STRINGi10116.ENSRNOP00000039247.

PTM databases

PhosphoSiteiP81799.

Proteomic databases

PRIDEiP81799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000042875; ENSRNOP00000039247; ENSRNOG00000013911.
GeneIDi297393.
KEGGirno:297393.
UCSCiRGD:1305057. rat.

Organism-specific databases

CTDi55577.
RGDi1305057. Nagk.

Phylogenomic databases

eggNOGiCOG2971.
GeneTreeiENSGT00510000047418.
HOGENOMiHOG000007248.
HOVERGENiHBG052570.
InParanoidiP81799.
KOiK00884.
OMAiLLPMDYS.
OrthoDBiEOG7V1FR7.
PhylomeDBiP81799.
TreeFamiTF314158.

Enzyme and pathway databases

UniPathwayiUPA00629.
SABIO-RKP81799.

Miscellaneous databases

NextBioi642192.
PROiP81799.

Gene expression databases

GenevestigatoriP81799.

Family and domain databases

InterProiIPR002731. ATPase_BadF.
[Graphical view]
PfamiPF01869. BcrAD_BadFG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  2. "Purification and characterization of N-acetylglucosamine kinase from rat liver. Comparison with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase."
    Hinderlich S., Noehring S., Weise C., Franke P., Staesche R., Reutter W.
    Eur. J. Biochem. 252:133-139(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-13; 69-92; 166-178; 198-207; 221-228; 233-235 AND 308-321, ACETYLATION AT ALA-2, ENZYME REGULATION, SUBUNIT, FUNCTION.
    Strain: Wistar.
    Tissue: Liver.

Entry informationi

Entry nameiNAGK_RAT
AccessioniPrimary (citable) accession number: P81799
Secondary accession number(s): Q32Q91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 74 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.