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Protein

N-acetyl-D-glucosamine kinase

Gene

Nagk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.1 Publication

Catalytic activityi

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.

Enzyme regulationi

Inhibited by the cysteine modifiers iodoacetamide, N-ethylmaleimide and 5,5'-dithiobis(2-nitrobenzoic acid).1 Publication

Pathwayi: N-acetylneuraminate degradation

This protein is involved in the pathway N-acetylneuraminate degradation, which is part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenBy similarity1
Binding sitei36SubstrateBy similarity1
Binding sitei107SubstrateBy similarity1
Binding sitei127ATPBy similarity1
Binding sitei152SubstrateBy similarity1
Binding sitei214ATP; via carbonyl oxygenBy similarity1
Binding sitei271ATPBy similarity1
Binding sitei275ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-446210. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKP81799.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyl-D-glucosamine kinase (EC:2.7.1.59)
Short name:
N-acetylglucosamine kinase
Alternative name(s):
GlcNAc kinase
Gene namesi
Name:Nagk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1305057. Nagk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000966982 – 343N-acetyl-D-glucosamine kinaseAdd BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei76PhosphoserineCombined sources1
Modified residuei205PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP81799.
PRIDEiP81799.

PTM databases

iPTMnetiP81799.
PhosphoSitePlusiP81799.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013911.
GenevisibleiP81799. RN.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP81799. 1 interactor.
STRINGi10116.ENSRNOP00000039247.

Structurei

3D structure databases

ProteinModelPortaliP81799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 130Substrate bindingBy similarity2
Regioni145 – 147Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1794. Eukaryota.
COG2971. LUCA.
GeneTreeiENSGT00510000047418.
HOGENOMiHOG000007248.
HOVERGENiHBG052570.
InParanoidiP81799.
KOiK00884.
OMAiPVEKTWA.
OrthoDBiEOG091G0LXR.
PhylomeDBiP81799.
TreeFamiTF314158.

Family and domain databases

InterProiIPR002731. ATPase_BadF.
[Graphical view]
PfamiPF01869. BcrAD_BadFG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALYGGVEG GGTRSKVLLL SEDGQILAEA DGLSTNHWLI GTGTCVERIN
60 70 80 90 100
EMVDRAKRKA GVDPLVPLRS LGLSLSGGEQ EDAVRLLMEE LRDRFPYLSE
110 120 130 140 150
SYFITTDAAG SIATATPDGG IVLISGTGSN CRLINPDGSE SGCGGWGHMM
160 170 180 190 200
GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD IGHVKQAMFN YFQVPDRLGI
210 220 230 240 250
LTHLYRDFDK SKFAGFCQKI AEGAQQGDPL SRFIFRKAGE MLGRHVVAVL
260 270 280 290 300
PEIDPVLFQG ELGLPILCVG SVWKSWELLK EGFLLALTQG REQQAQNSFS
310 320 330 340
SFTLMKLRHS SALGGASLGA RHIGHHLPMD YSVNAIAFYS YTF
Length:343
Mass (Da):37,196
Last modified:January 23, 2007 - v4
Checksum:i43886E3262D08A0F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207D → T AA sequence (PubMed:9523722).Curated1
Sequence conflicti226Q → E AA sequence (PubMed:9523722).Curated1
Sequence conflicti309H → I AA sequence (PubMed:9523722).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107647 mRNA. Translation: AAI07648.1.
RefSeqiNP_001032857.1. NM_001037768.1.
UniGeneiRn.105985.

Genome annotation databases

EnsembliENSRNOT00000042875; ENSRNOP00000039247; ENSRNOG00000013911.
GeneIDi297393.
KEGGirno:297393.
UCSCiRGD:1305057. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107647 mRNA. Translation: AAI07648.1.
RefSeqiNP_001032857.1. NM_001037768.1.
UniGeneiRn.105985.

3D structure databases

ProteinModelPortaliP81799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP81799. 1 interactor.
STRINGi10116.ENSRNOP00000039247.

PTM databases

iPTMnetiP81799.
PhosphoSitePlusiP81799.

Proteomic databases

PaxDbiP81799.
PRIDEiP81799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000042875; ENSRNOP00000039247; ENSRNOG00000013911.
GeneIDi297393.
KEGGirno:297393.
UCSCiRGD:1305057. rat.

Organism-specific databases

CTDi55577.
RGDi1305057. Nagk.

Phylogenomic databases

eggNOGiKOG1794. Eukaryota.
COG2971. LUCA.
GeneTreeiENSGT00510000047418.
HOGENOMiHOG000007248.
HOVERGENiHBG052570.
InParanoidiP81799.
KOiK00884.
OMAiPVEKTWA.
OrthoDBiEOG091G0LXR.
PhylomeDBiP81799.
TreeFamiTF314158.

Enzyme and pathway databases

UniPathwayiUPA00629.
ReactomeiR-RNO-446210. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKP81799.

Miscellaneous databases

PROiP81799.

Gene expression databases

BgeeiENSRNOG00000013911.
GenevisibleiP81799. RN.

Family and domain databases

InterProiIPR002731. ATPase_BadF.
[Graphical view]
PfamiPF01869. BcrAD_BadFG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAGK_RAT
AccessioniPrimary (citable) accession number: P81799
Secondary accession number(s): Q32Q91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 86 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.