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P81795 (IF2G_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 2 subunit 3
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit gamma
Short name=eIF-2-gamma
PP42
Gene names
Name:Eif2s3
Synonyms:Eif2g
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Subunit structure

Heterotrimer composed of an alpha, a beta and a gamma chain.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EIF2G subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 472471Eukaryotic translation initiation factor 2 subunit 3
PRO_0000137443

Regions

Nucleotide binding48 – 558GTP By similarity
Nucleotide binding134 – 1385GTP By similarity
Nucleotide binding190 – 1934GTP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue161Phosphoserine By similarity

Experimental info

Sequence conflict351V → I in AAI58737. Ref.1
Sequence conflict941P → S in AAI58737. Ref.1
Sequence conflict1151T → S in AAI58737. Ref.1
Sequence conflict1251K → R in AAI58737. Ref.1
Sequence conflict4691V → I in AAI58737. Ref.1
Sequence conflict4721D → E in AAI58737. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P81795 [UniParc].

Last modified February 10, 2009. Version 2.
Checksum: 9C66F42862AFD722

FASTA47251,079
        10         20         30         40         50         60 
MAGGEAGVTL GQPHLSRQDL ATLDVTKLTP LSHEVISRQA TINIGTIGHV AHGKSTVVKA 

        70         80         90        100        110        120 
ISGVHTVRFK NELERNITIK LGYANAKIYK LDDPSCPRPE CYRSCGSSTP DEFPTDIPGT 

       130        140        150        160        170        180 
KGNFKLVRHV SFVDCPGHDI LMATMLNGAA VMDAALLLIA GNESCPQPQT SEHLAAIEIM 

       190        200        210        220        230        240 
KLKHILILQN KIDLVKESQA KEQYEQILAF VQGTVAEGAP IIPISAQLKY NIEVVCEYIV 

       250        260        270        280        290        300 
KKIPVPPRDF TSEPRLIVIR SFDVNKPGCE VDDLKGGVAG GSILKGVLKV GQEIEVRPGI 

       310        320        330        340        350        360 
VSKDSEGKLM CKPIFSKIVS LFAEHNDLQY AAPGGLIGVG TKIDPTLCRA DRMVGQVLGA 

       370        380        390        400        410        420 
VGALPEIFTE LEISYFLLRR LLGVRTEGDK KAAKVQKLSK NEVLMVNIGS LSTGGRVSAV 

       430        440        450        460        470 
KADLGKIVLT NPVCTEVGEK IALSRRVEKH WRLIGWGQIR RGVTIKPTVD DD 

« Hide

References

« Hide 'large scale' references
[1]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary, Prostate and Spleen.
[3]"Rat liver pp49, a protein that forms complexes with protein kinase CK2, is composed of the beta and the gamma subunits of translation initiation factor eIF-2."
Gil C., Plana M., Riera M., Itarte E.
Biochem. Biophys. Res. Commun. 225:1052-1057(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 290-303.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH473966 Genomic DNA. Translation: EDL96007.1.
BC091286 mRNA. Translation: AAH91286.1.
BC158736 mRNA. Translation: AAI58737.1.
BC166783 mRNA. Translation: AAI66783.1.
RefSeqNP_001094012.1. NM_001100542.1.
NP_001161138.1. NM_001167666.1.
UniGeneRn.220237.
Rn.87025.

3D structure databases

ProteinModelPortalP81795.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid256094. 1 interaction.
STRING10116.ENSRNOP00000008489.

Proteomic databases

PaxDbP81795.
PRIDEP81795.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000058134; ENSRNOP00000054939; ENSRNOG00000006186.
GeneID100312984.
299027.
KEGGrno:100312984.
rno:299027.
UCSCRGD:2314438. rat.

Organism-specific databases

CTD26905.
26908.

Phylogenomic databases

eggNOGCOG5257.
GeneTreeENSGT00550000074801.
HOGENOMHOG000229292.
HOVERGENHBG006123.
InParanoidQ5BJX8.
KOK03242.
OMACPQPQTK.
OrthoDBEOG7H1JK8.
PhylomeDBP81795.
TreeFamTF101513.

Gene expression databases

GenevestigatorP81795.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR015256. TIF2_gsu_C.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF09173. eIF2_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

NextBio644677.
PROP81795.

Entry information

Entry nameIF2G_RAT
AccessionPrimary (citable) accession number: P81795
Secondary accession number(s): B0BN96, B2RYH8, Q5BJX8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 10, 2009
Last modified: April 16, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries