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Protein

NAD-dependent alcohol dehydrogenase

Gene
N/A
Organism
Moraxella sp. (strain TAE123)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

A psychrophilic enzyme that oxidizes mainly primary and secondary aliphatic alcohols, as well as large bulky alcohols. Also catalyzes the reduction of ketones and aldehydes but in lower rates than the oxidations. The enzyme reduces stereospecifically 2-butanone to produce (s)-2-butanol.Curated

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Enzyme regulationi

The presence of Ca2+ results in a considerable stabilization of the enzyme.

Kineticsi

Highest reaction rate when ethanol is used as the substrate.

      pH dependencei

      Optimum pH is 7.5.

      Temperature dependencei

      Thermolabile.

      GO - Molecular functioni

      • alcohol dehydrogenase (NAD) activity Source: UniProtKB-EC
      • alcohol dehydrogenase (NADP+) activity Source: UniProtKB
      • zinc ion binding Source: UniProtKB

      GO - Biological processi

      • alcohol metabolic process Source: UniProtKB

      Keywordsi

      Molecular functionOxidoreductase
      LigandMetal-binding, NAD, Zinc

      Enzyme and pathway databases

      SABIO-RKiP81786.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      NAD-dependent alcohol dehydrogenase (EC:1.1.1.1)
      Short name:
      ADH
      OrganismiMoraxella sp. (strain TAE123)
      Taxonomic identifieri191545 [NCBI]
      Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      ChainiPRO_00001607411 – ›23NAD-dependent alcohol dehydrogenaseAdd BLAST›23

      Interactioni

      Subunit structurei

      Homotetramer.Curated

      Family & Domainsi

      Sequence similaritiesi

      Sequencei

      Sequence statusi: Fragment.

      P81786-1 [UniParc]FASTAAdd to basket

      « Hide

              10         20 
      MLYANPNTES SPVGFRKKYD NFI
      Length:23
      Mass (Da):2,692
      Last modified:June 1, 2001 - v1
      Checksum:i3BA7AA2A2EAE5D84
      GO

      Experimental Info

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Non-terminal residuei23Curated1

      Similar proteinsi

      Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
      100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
      90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
      50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

      Entry informationi

      Entry nameiADH_MORSE
      AccessioniPrimary (citable) accession number: P81786
      Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
      Last sequence update: June 1, 2001
      Last modified: May 27, 2015
      This is version 34 of the entry and version 1 of the sequence. See complete history.
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Direct protein sequencing

      Documents

      1. SIMILARITY comments
        Index of protein domains and families