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Protein

Beta-lactamase CARB-6

Gene

carB6

Organism
Vibrio cholerae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes both ticarcillin and oxacillin.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by clavulanic acid, sulbactam and tazobactam.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase CARB-6 (EC:3.5.2.6)
Alternative name(s):
Carbenicillinase 6
Gene namesi
Name:carB6
Encoded oniPlasmid0 Publication
OrganismiVibrio cholerae
Taxonomic identifieri666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 288271Beta-lactamase CARB-6PRO_0000017045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 118Sequence Analysis

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP81781.
SMRiP81781. Positions 20-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2313Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG
60 70 80 90 100
EYWDYNGNQR FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY
110 120 130 140 150
SPVIEKQVGQ AITLDDACFA TMTTSDNTAA NIILSAVGGP KGVTDFLRQI
160 170 180 190 200
GDKETRLDRI EPDLNEGKLG DLRDTTTPKA IASTLNQLLF GSTLSEASQK
210 220 230 240 250
KLESWMVNNQ VTGNLLRSVL PVKWSIADRS GAGGFGARSI TAIVWSEEKK
260 270 280
TIIVSIYLAQ TEASMAERND AIVKIGRSIF EVYTSQSR
Length:288
Mass (Da):31,430
Last modified:May 1, 1999 - v1
Checksum:iFBBE71A6AB090928
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030945 Genomic DNA. Translation: AAD19217.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030945 Genomic DNA. Translation: AAD19217.1.

3D structure databases

ProteinModelPortaliP81781.
SMRiP81781. Positions 20-285.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization and nucleotide sequence of CARB-6, a new carbenicillin-hydrolyzing beta-lactamase from Vibrio cholerae."
    Choury D., Aubert G., Szajnert M.-F., Azibi K., Delpech M., Paul G.
    Antimicrob. Agents Chemother. 43:297-301(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: NON-O1 and NON-O139.

Entry informationi

Entry nameiBLC6_VIBCL
AccessioniPrimary (citable) accession number: P81781
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: October 1, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.