P81781 (BLC6_VIBCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase CARB-6
EC=3.5.2.6

Alternative name(s):
Carbenicillinase 6

Gene names

Name:

carB6

Encoded on

Plasmid

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes both ticarcillin and oxacillin.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Enzyme regulation	Inhibited by clavulanic acid, sulbactam and tazobactam.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 288	271	Beta-lactamase CARB-6		PRO_0000017045
Regions
	Region	229 – 231	3	Substrate binding By similarity
Sites
	Active site	65	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Disulfide bond	72 ↔ 118		Potential

Sequences

	Sequence		Length	Mass (Da)
	P81781 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: FBBE71A6AB090928	FASTA	288	31,430
	10 20 30 40 50 60 MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG EYWDYNGNQR 70 80 90 100 110 120 FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY SPVIEKQVGQ AITLDDACFA 130 140 150 160 170 180 TMTTSDNTAA NIILSAVGGP KGVTDFLRQI GDKETRLDRI EPDLNEGKLG DLRDTTTPKA 190 200 210 220 230 240 IASTLNQLLF GSTLSEASQK KLESWMVNNQ VTGNLLRSVL PVKWSIADRS GAGGFGARSI 250 260 270 280 TAIVWSEEKK TIIVSIYLAQ TEASMAERND AIVKIGRSIF EVYTSQSR

References

[1]

"Characterization and nucleotide sequence of CARB-6, a new carbenicillin-hydrolyzing beta-lactamase from Vibrio cholerae." Choury D., Aubert G., Szajnert M.-F., Azibi K., Delpech M., Paul G. Antimicrob. Agents Chemother. 43:297-301(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: NON-O1 and NON-O139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF030945 Genomic DNA. Translation: AAD19217.1.
3D structure databases
ProteinModelPortal	P81781.
SMR	P81781. Positions 20-285.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLC6_VIBCL

Accession

Primary (citable) accession number: P81781

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program