Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P81762

- RNPO_PLEOS

UniProt

P81762 - RNPO_PLEOS

Protein

Guanyl-specific ribonuclease Po1

Gene
N/A
Organism
Pleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

    Enzyme regulationi

    Inhibited by divalent cations. Inhibition decreases in the order zinc, lead, cadmium, nickel, mercury.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei36 – 361By similarity
    Active sitei54 – 541Proton acceptorBy similarity
    Active sitei87 – 871Proton donorBy similarity

    GO - Molecular functioni

    1. endoribonuclease activity Source: InterPro
    2. ribonuclease T1 activity Source: UniProtKB-EC
    3. RNA binding Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanyl-specific ribonuclease Po1 (EC:3.1.27.3)
    Short name:
    RNase Po1
    OrganismiPleurotus ostreatus (Oyster mushroom) (White-rot fungus)
    Taxonomic identifieri5322 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPleurotaceaePleurotus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 101101Guanyl-specific ribonuclease Po1PRO_0000137374Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11Pyrrolidone carboxylic acid
    Disulfide bondi7 ↔ 84Sequence Analysis
    Disulfide bondi9 ↔ 99By similarity
    Disulfide bondi48 ↔ 82By similarity

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Structurei

    3D structure databases

    ProteinModelPortaliP81762.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribonuclease N1/T1 family.Curated

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P81762-1 [UniParc]FASTAAdd to Basket

    « Hide

    QTGVRSCNCA GRSFTGTDVT NAIRSARAGG SGNYPHVYNN FEGFSFSCTP    50
    TFFEFPVFRG SVYSGGSPGA DRVIYDQSGR FCACLTHTGA PSTNGFVECR 100
    F 101
    Length:101
    Mass (Da):10,789
    Last modified:June 1, 2001 - v1
    Checksum:iFF30477497990D00
    GO

    Sequence databases

    PIRiJX0333.

    Cross-referencesi

    Sequence databases

    PIRi JX0333.

    3D structure databases

    ProteinModelPortali P81762.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification and primary structure of a new guanylic acid specific ribonuclease from Pleurotus ostreatus."
      Nomura H., Inokuchi N., Kobayashi H., Koyama T., Iwama M., Ohgi K., Irie M.
      J. Biochem. 116:26-33(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, CHARACTERIZATION.

    Entry informationi

    Entry nameiRNPO_PLEOS
    AccessioniPrimary (citable) accession number: P81762
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3