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P81762

- RNPO_PLEOS

UniProt

P81762 - RNPO_PLEOS

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Protein

Guanyl-specific ribonuclease Po1

Gene
N/A
Organism
Pleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Enzyme regulationi

Inhibited by divalent cations. Inhibition decreases in the order zinc, lead, cadmium, nickel, mercury.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361 By similarity
Active sitei54 – 541Proton acceptor By similarity
Active sitei87 – 871Proton donor By similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. ribonuclease T1 activity Source: UniProtKB-EC
  3. RNA binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease Po1 (EC:3.1.27.3)
Short name:
RNase Po1
OrganismiPleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Taxonomic identifieri5322 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPleurotaceaePleurotus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 101101Guanyl-specific ribonuclease Po1PRO_0000137374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11Pyrrolidone carboxylic acid
Disulfide bondi7 ↔ 84 Reviewed prediction
Disulfide bondi9 ↔ 99 By similarity
Disulfide bondi48 ↔ 82 By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Structurei

3D structure databases

ProteinModelPortaliP81762.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

P81762-1 [UniParc]FASTAAdd to Basket

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QTGVRSCNCA GRSFTGTDVT NAIRSARAGG SGNYPHVYNN FEGFSFSCTP    50
TFFEFPVFRG SVYSGGSPGA DRVIYDQSGR FCACLTHTGA PSTNGFVECR 100
F 101
Length:101
Mass (Da):10,789
Last modified:June 1, 2001 - v1
Checksum:iFF30477497990D00
GO

Sequence databases

PIRiJX0333.

Cross-referencesi

Sequence databases

PIRi JX0333.

3D structure databases

ProteinModelPortali P81762.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Purification and primary structure of a new guanylic acid specific ribonuclease from Pleurotus ostreatus."
    Nomura H., Inokuchi N., Kobayashi H., Koyama T., Iwama M., Ohgi K., Irie M.
    J. Biochem. 116:26-33(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, CHARACTERIZATION.

Entry informationi

Entry nameiRNPO_PLEOS
AccessioniPrimary (citable) accession number: P81762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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