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P81762 (RNPO_PLEOS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanyl-specific ribonuclease Po1

Short name=RNase Po1
EC=3.1.27.3
OrganismPleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Taxonomic identifier5322 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPleurotaceaePleurotus

Protein attributes

Sequence length101 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Enzyme regulation

Inhibited by divalent cations. Inhibition decreases in the order zinc, lead, cadmium, nickel, mercury.

Sequence similarities

Belongs to the ribonuclease N1/T1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 101101Guanyl-specific ribonuclease Po1
PRO_0000137374

Sites

Active site361 By similarity
Active site541Proton acceptor By similarity
Active site871Proton donor By similarity

Amino acid modifications

Modified residue11Pyrrolidone carboxylic acid
Disulfide bond7 ↔ 84 Potential
Disulfide bond9 ↔ 99 By similarity
Disulfide bond48 ↔ 82 By similarity

Sequences

Sequence LengthMass (Da)Tools
P81762 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: FF30477497990D00

FASTA10110,789
        10         20         30         40         50         60 
QTGVRSCNCA GRSFTGTDVT NAIRSARAGG SGNYPHVYNN FEGFSFSCTP TFFEFPVFRG 

        70         80         90        100 
SVYSGGSPGA DRVIYDQSGR FCACLTHTGA PSTNGFVECR F 

« Hide

References

[1]"Purification and primary structure of a new guanylic acid specific ribonuclease from Pleurotus ostreatus."
Nomura H., Inokuchi N., Kobayashi H., Koyama T., Iwama M., Ohgi K., Irie M.
J. Biochem. 116:26-33(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, CHARACTERIZATION.

Cross-references

Sequence databases

PIRJX0333.

3D structure databases

ProteinModelPortalP81762.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.450.30. 1 hit.
InterProIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMSSF53933. SSF53933. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRNPO_PLEOS
AccessionPrimary (citable) accession number: P81762
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families