P81762 (RNPO_PLEOS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Guanyl-specific ribonuclease Po1 Short name=RNase Po1 EC=3.1.27.3
Organism	Pleurotus ostreatus (Oyster mushroom) (White-rot fungus)
Taxonomic identifier	5322 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Agaricomycetidae › Agaricales › Pleurotaceae › Pleurotus

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.
Enzyme regulation	Inhibited by divalent cations. Inhibition decreases in the order zinc, lead, cadmium, nickel, mercury.
Sequence similarities	Belongs to the ribonuclease N1/T1 family.

Ontologies

Keywords
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro ribonuclease T1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 101	101	Guanyl-specific ribonuclease Po1		PRO_0000137374
Sites
	Active site	36	1	By similarity
	Active site	54	1	Proton acceptor By similarity
	Active site	87	1	Proton donor By similarity
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid
	Disulfide bond	7 ↔ 84		Potential
	Disulfide bond	9 ↔ 99		By similarity
	Disulfide bond	48 ↔ 82		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81762 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: FF30477497990D00	FASTA	101	10,789
	10 20 30 40 50 60 QTGVRSCNCA GRSFTGTDVT NAIRSARAGG SGNYPHVYNN FEGFSFSCTP TFFEFPVFRG 70 80 90 100 SVYSGGSPGA DRVIYDQSGR FCACLTHTGA PSTNGFVECR F

References

[1]	"Purification and primary structure of a new guanylic acid specific ribonuclease from Pleurotus ostreatus." Nomura H., Inokuchi N., Kobayashi H., Koyama T., Iwama M., Ohgi K., Irie M. J. Biochem. 116:26-33(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, CHARACTERIZATION.

Cross-references

Sequence databases
PIR	JX0333.
3D structure databases
ProteinModelPortal	P81762.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.10.450.30. 1 hit.
InterPro	IPR000026. Gua-sp_ribonuclease_N1/T1. IPR016191. Ribonuclease/ribotoxin. [Graphical view]
Pfam	PF00545. Ribonuclease. 1 hit. [Graphical view]
SUPFAM	SSF53933. Ribonuclease/ribotoxin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNPO_PLEOS

Accession

Primary (citable) accession number: P81762

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program