Reviewed, UniProtKB/Swiss-Prot P81760 (TL17_ARATH)

Names and origin

Protein names

Recommended name:
    Thylakoid lumenal 17.4 kDa protein, chloroplastic
Alternative name(s):
    P17.4

Gene names

Ordered Locus Names:	At5g53490
ORF Names:	MNC6.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Plastid › chloroplast thylakoid lumen.
Sequence similarities	Contains 2 pentapeptide repeat domains.

Ontologies

Keywords
Cellular component	Chloroplast Plastid Thylakoid
Coding sequence diversity	Alternative splicing
Domain	Repeat Transit peptide
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR chloroplast thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449573,EBI-449157

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P81760-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P81760-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-36: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Transit peptide	? – 77		Thylakoid
	Chain	78 – 236	159	Thylakoid lumenal 17.4 kDa protein, chloroplastic		PRO_0000023524
Regions
	Domain	124 – 163	40	Pentapeptide repeat 1
	Domain	169 – 208	40	Pentapeptide repeat 2
Natural variations
	Alternative sequence	36	1	Missing in isoform 2.		VSP_034389
Experimental info
	Sequence conflict	21	1	N → K Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 21, 2003. Version 2. Checksum: D14502C4372A3A14	FASTA	236	25,644
	10 20 30 40 50 60 MASLPVQFTR NQISSPFFSV NLRREPRSLV TVHCSAGENR ENGEGVKKSL FPLKELGSIA 70 80 90 100 110 120 CAALCACTLT IASPVIAANQ RLPPLSTEPD RCEKAFVGNT IGQANGVYDK PLDLRFCDYT 130 140 150 160 170 180 NDQTNLKGKT LSAALMVGAK FDGADMTEVV MSKAYAVEAS FKGVNFTNAV IDRVNFGKSN 190 200 210 220 230 LKGAVFRNTV LSGSTFEEAN LEDVVFEDTI IGYIDLQKIC RNESINEEGR LVLGCR
	Isoform 2. Checksum: 64733A4AD86B0E81	FASTA	235	25,573
	10 20 30 40 50 60 MASLPVQFTR NQISSPFFSV NLRREPRSLV TVHCSGENRE NGEGVKKSLF PLKELGSIAC 70 80 90 100 110 120 AALCACTLTI ASPVIAANQR LPPLSTEPDR CEKAFVGNTI GQANGVYDKP LDLRFCDYTN 130 140 150 160 170 180 DQTNLKGKTL SAALMVGAKF DGADMTEVVM SKAYAVEASF KGVNFTNAVI DRVNFGKSNL 190 200 210 220 230 KGAVFRNTVL SGSTFEEANL EDVVFEDTII GYIDLQKICR NESINEEGRL VLGCR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation of an Arabidopsis cDNA encoding a thylakoid lumen protein related to a novel 'pentapeptide repeat' family of proteins." Kieselbach T., Mant A., Robinson C., Schroeder W.P. FEBS Lett. 428:241-244(1998) [PubMed: 9654141] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction." Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G., Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G., van Wijk K.J. Plant Cell 14:211-236(2002) [PubMed: 11826309] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY.
[5]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed: 18431481] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB015476 Genomic DNA. Translation: BAB09725.1. AF370552 mRNA. Translation: AAK48979.1. BT003410 mRNA. Translation: AAO30073.1.
IPI	IPI00524300. IPI00528484.
RefSeq	NP_200161.2. NP_851183.1.
UniGene	At.20426 At.70747
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P81760. 1 interaction.
Proteomic databases
PRIDE	P81760.
Genome annotation databases
GeneID	835431.
GenomeReviews	Gene locus AT5G53490 in contig BA000015_GR.
KEGG	ath:AT5G53490.
NMPDR	fig|3702.1.peg.27245.
Organism-specific databases
TAIR	At5g53490.
Phylogenomic databases
OMA	P81760. FVGNTIG.
Gene expression databases
ArrayExpress	P81760.
Family and domain databases
InterPro	IPR001646. 5peptide_repeat. [Graphical view]
Pfam	PF00805. Pentapeptide. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TL17_ARATH

Accession

Primary (citable) accession number: P81760
Secondary accession number(s): Q3E8C0, Q93Y82

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 21, 2003
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)