Reviewed, UniProtKB/Swiss-Prot P81748 (MIFH_TRITR)

Names and origin

Protein names	Recommended name:     Macrophage migration inhibitory factor homolog       Short name=MIF     EC=5.3.2.1 Alternative name(s):     Phenylpyruvate tautomerase     L-dopachrome tautomerase     EC=5.3.3.12     L-dopachrome isomerase
Organism	Trichuris trichiura (Whipworm)
Taxonomic identifier	36087 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Nematoda › Enoplea › Trichocephalida › Trichuridae › Trichuris

Protein attributes

Sequence length	114 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tautomerization of the methyl ester of L-dopachrome. Inhibits migration of human peripheral blood mononuclear cells By similarity.
Catalytic activity	L-dopachrome = 5,6-dihydroxyindole-2-carboxylate. Ref.1 Keto-phenylpyruvate = enol-phenylpyruvate. Ref.1
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the MIF family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Cytokine Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW dopachrome isomerase activity Inferred from electronic annotation. Source: EC phenylpyruvate tautomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 114	113	Macrophage migration inhibitory factor homolog		PRO_0000158076
Sites
	Active site	2	1	Proton acceptor; via imino nitrogen By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81748-1 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 43B3BD9A9C77215B	FASTA	114	12,043
	10 20 30 40 50 60 MPIFTFSTNV PSENISVDFL KSTSKLIAGM LGKPESYVAV HINGGQKITF GGTDAPAGFG 70 80 90 100 110 QLLSLGGVGG EKNRSHSAKL FKHLTDGLGI PGNRMYINFV DMRGSDVGYN GSTF

References

[1]

"Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis." Tan T.H.P., Edgerton S.A.V., Kumari R., McAlister M.S.B., Roe S.M., Nagl S., Pearl L.H., Selkirk M.E., Bianco A.E., Totty N.F., Engwerda C., Gray C.A., Meyer D.J., Rowe S.M. Biochem. J. 357:373-383(2001) [PubMed: 11439086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-34; 48-69 AND 80-109, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ237770 mRNA. Translation: CAB46355.1.
3D structure databases
SMR	P81748. Positions 1-114.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.3.3.12. 301024.
Family and domain databases
InterPro	IPR001398. Macrophage_inhib_fac. IPR014347. Tautomerase. [Graphical view]
Gene3D	G3DSA:3.30.429.10. Tautomerase. 1 hit.
PANTHER	PTHR11954. MIF. 1 hit.
Pfam	PF01187. MIF. 1 hit. [Graphical view]
ProDom	PD004816. Macrophage_inhib_fac. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01158. MIF. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIFH_TRITR

Accession

Primary (citable) accession number: P81748
Secondary accession number(s): Q9U920

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	September 22, 2009
Last modified:	January 19, 2010
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)