Reviewed, UniProtKB/Swiss-Prot P81731 (AMPN_HELAM)

Names and origin

Protein names	Recommended name:     Aminopeptidase N       Short name=AP-N     EC=3.4.11.2 Alternative name(s):     CryIA(C) receptor
Organism	Helicoverpa armigera (Cotton bollworm) (Heliothis armigera)
Taxonomic identifier	29058 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Noctuoidea › Noctuidae › Heliothinae › Helicoverpa

Protein attributes

Sequence length	10 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a receptor for B.thuringiensis Cry1Ac delta-endotoxin. Ref.1
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide. Ref.1
Cofactor	Binds 1 zinc ion per subunit By similarity.
Enzyme regulation	Inhibited by the zinc-chelators 2,2-dipyridyl and 1,10-phenanthroline. Ref.1
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Ligand	Zinc
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›10	›10	Aminopeptidase N		PRO_0000095086
Experimental info
	Non-terminal residue	10	1

Sequences

	Sequence		Length	Mass (Da)
	P81731-1 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: 05042EB87B11F1BB	FASTA	10	1,093
	10 GMYTHEGSDP

References

[1]

"Aminopeptidase-N from the Helicoverpa armigera (Hubner) brush border membrane vesicles as a receptor of Bacillus thuringiensis crylac delta-endotoxin." Ingle S.S., Trivedi N., Prasad R., Kuruvilla J., Rao K.K., Chhatpar H.S. Curr. Microbiol. 43:255-259(2001) [PubMed: 11683359] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION. Tissue: Larval midgut.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.11.2. 39282.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPN_HELAM

Accession

Primary (citable) accession number: P81731

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	October 13, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)