Tyrosine-protein phosphatase non-receptor type 6

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P81718 (PTN6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 6
EC=3.1.3.48

Alternative name(s):
Protein-tyrosine phosphatase SHP-1

Gene names

Name:	Ptpn6
Synonyms:	Ptph6

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis. Ref.2
Catalytic activity	Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate. UniProtKB P29351
Subunit structure	Monomer. Binds PTPNS1. Interacts with CDK2, FCRL2, FCRL3, FCRL4, CD300LF and CD84. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN By similarity. Interacts with MTUS1. Interacts with the tyrosine phosphorylated form of PDPK1 By similarity. Ref.2
Subcellular location	Cytoplasm. Nucleus. Note: In neurons, translocates into the nucleus after treatment with angiotensin II. Shuttles between the cytoplasm and nucleus via its association with PDPK By similarity. Ref.2
Developmental stage	Highly expressed in embryonic cerebral cortex between day 18 and up to birth. Expression levels decrease after birth (at protein level). Ref.2
Domain	The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation By similarity.
Post-translational modification	Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation By similarity. Phosphorylation at Tyr-566 enhances phosphatase activity By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily. Contains 2 SH2 domains. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat SH2 domain
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB regulation of ERK1 and ERK2 cascade Inferred from sequence or structural similarity. Source: UniProtKB response to wounding Inferred from expression pattern PubMed 11157073. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transmembrane receptor protein tyrosine phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 613

613

Tyrosine-protein phosphatase non-receptor type 6

PRO_0000094760

Regions

Domain

6 – 102

SH2 1

Domain

112 – 215

104

SH2 2

Domain

246 – 517

272

Tyrosine-protein phosphatase

Region

455 – 461

Substrate binding By similarity

Sites

Active site

455

Phosphocysteine intermediate By similarity UniProtKB P29350

Binding site

421

Substrate By similarity

Binding site

502

Substrate By similarity

Amino acid modifications

Modified residue

Phosphotyrosine By similarity

Modified residue

379

Phosphotyrosine By similarity

Modified residue

538

Phosphotyrosine By similarity

Modified residue

566

Phosphotyrosine; by LYN By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P81718 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 29364B22E8F45C87
Show »

FASTA

613

69,578

        10         20         30         40         50         60 
MLSRGWFHRD LSGPDAETLL KGRGVPGSFL ARPSRKNQGD FSLSVRVDDQ VTHIRIQNSG 

        70         80         90        100        110        120 
DFYDLYGGEK FATSTELVEY YTQQQGILQD RDGTIIHLKY PLNCSDPTSE RWYHGHMSGG 

       130        140        150        160        170        180 
QAESLLQAKG EPWTFLVRES LSQPGDFVLS VLNDQPKAAP GSPLRVTHIK VMCEGGRYTV 

       190        200        210        220        230        240 
GGSETFDSLT DLVEHFKKTG IEEASGAFVY LRQPYYATRV NAADIENRVL ELNKKQESED 

       250        260        270        280        290        300 
TAKAGFWEEF ESLQKQEAKN LHQRLEGQRP ENKSKNRYKN ILPFDHSRVI LQGRDSNIPG 

       310        320        330        340        350        360 
SDYINANYVK NQLLGPDENS KTYIASQGCL DATVNDFWQM AWQENTRVIV MTTREVEKGR 

       370        380        390        400        410        420 
NKCVPYWPEV GTQRVYGLYS VTNCKEHDTA EYKLRTLQIS PLDNGDLVRE IWHYQYLSWP 

       430        440        450        460        470        480 
DHGVPSEPGG VLSFLDQINQ RQESLPHAGP IIVHCSAGIG RTGTIIVIDM LMESVSTKGL 

       490        500        510        520        530        540 
DCDIDIQKTI QMVRAQRSGM VQTEAQYKFI YVAIAQFIET TKKKLEIIQS QRGQESEYGN 

       550        560        570        580        590        600 
ITYPPALRSA HAKASRTSSK HKEEVYENVH SKNKKEEKVK KQRSADKEKN KGSLKRNISL 

       610 
TPCRGLRWAD RDL

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References

[1]	"The rat SH2-containing protein-tyrosine phosphatase SHP-1 is a positive regulator of NGF-induced neuronal differentiation of PC12 cells." Aoki N., Yamaguchi-Aoki Y., Ullrich A. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Angiotensin II-induced neural differentiation via angiotensin II type 2 (AT2) receptor-MMS2 cascade involving interaction between AT2 receptor-interacting protein and Src homology 2 domain-containing protein-tyrosine phosphatase 1." Li J.-M., Mogi M., Tsukuda K., Tomochika H., Iwanami J., Min L.-J., Nahmias C., Iwai M., Horiuchi M. Mol. Endocrinol. 21:499-511(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INTERACTION WITH MTUS1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77038 mRNA. Translation: AAD00262.1.
IPI	IPI00339114.
RefSeq	NP_446360.1. NM_053908.1.
UniGene	Rn.18985.
3D structure databases
ProteinModelPortal	P81718.
SMR	P81718. Positions 6-528.
ModBase	Search...
PTM databases
PhosphoSite	P81718.
Proteomic databases
PaxDb	P81718.
PRIDE	P81718.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	116689.
KEGG	rno:116689.
UCSC	RGD:620660. rat.
Organism-specific databases
CTD	5777.
RGD	620660. Ptpn6.
Phylogenomic databases
eggNOG	COG5599.
HOGENOM	HOG000273907.
HOVERGEN	HBG000223.
InParanoid	P81718.
KO	K05697.
OrthoDB	EOG4255SK.
Gene expression databases
ArrayExpress	P81718.
Genevestigator	P81718.
GermOnline	ENSRNOG00000014294. Rattus norvegicus.
Family and domain databases
Gene3D	3.30.505.10. 2 hits.
InterPro	IPR000980. SH2. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR012152. Tyr_Pase_non-rcpt_typ-6/11. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00017. SH2. 2 hits. PF00102. Y_phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF000929. Tyr-Ptase_nr_6. 1 hit.
PRINTS	PR00700. PRTYPHPHTASE. PR00401. SH2DOMAIN.
SMART	SM00194. PTPc. 1 hit. SM00252. SH2. 2 hits. [Graphical view]
PROSITE	PS50001. SH2. 2 hits. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	619552.

Entry information

Entry name

PTN6_RAT

Accession

Primary (citable) accession number: P81718

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents