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P81717 (CWHA_ACHLY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
N-acetylmuramoyl-L-alanine amidase A

EC=3.5.1.28
Gene names
Name:cwhA
OrganismAchromobacter lyticus
Taxonomic identifier224 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAchromobacter

Protein attributes

Sequence length177 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Antibacterial activity against Gram-positive bacteria M.luteus, S.aureus, E.faecalis and P.acidilactici and Gram-negative bacterium E.coli. Ref.1

Catalytic activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Subcellular location

Secreted.

Biophysicochemical properties

pH dependence:

Optimum pH is 8.5.

Mass spectrometry

Molecular mass is 19395.9 Da from positions 1 - 177. Determined by MALDI. Ref.1

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentSecreted
   Molecular functionAntibiotic
Antimicrobial
Hydrolase
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdefense response to bacterium

Inferred from electronic annotation. Source: UniProtKB-KW

peptidoglycan catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionN-acetylmuramoyl-L-alanine amidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 177177N-acetylmuramoyl-L-alanine amidase A
PRO_0000079618

Regions

Compositional bias172 – 1754Poly-Gly

Amino acid modifications

Disulfide bond114 ↔ 121 Ref.1

Sequences

Sequence LengthMass (Da)Tools
P81717 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 040D7F5D806C9C53

FASTA17719,395
        10         20         30         40         50         60 
AVDFGEAIWN PASSSNYSTA SNQTSAVIMH TMEGSYAGSI SWFQNPSAQV SAHYLIRKSD 

        70         80         90        100        110        120 
GQITQMVREY HQAWHAKNHN YYTIGIEHDG RAADAGNWSA AMVNASARLT KSICARRGVN 

       130        140        150        160        170 
CASAWKGPGY DTFHLVPDSV RVKGHGMLSG NENRYDPGKY FPWSNYYNLI NGGGGNP 

« Hide

References

[1]"Purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, CwhA, from Achromobacter lyticus."
Li S., Norioka S., Sakiyama F.
J. Biochem. 127:1033-1039(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, DISULFIDE BOND.
Strain: M497-1.

Cross-references

Sequence databases

PIRJC7276.

3D structure databases

ProteinModelPortalP81717.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA3.5.1.28. 74.

Family and domain databases

Gene3D3.40.80.10. 1 hit.
InterProIPR002502. Amidase_domain.
[Graphical view]
PfamPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMSSF55846. SSF55846. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCWHA_ACHLY
AccessionPrimary (citable) accession number: P81717
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program