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P81717

- CWHA_ACHLY

UniProt

P81717 - CWHA_ACHLY

Protein

N-acetylmuramoyl-L-alanine amidase A

Gene

cwhA

Organism
Achromobacter lyticus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Antibacterial activity against Gram-positive bacteria M.luteus, S.aureus, E.faecalis and P.acidilactici and Gram-negative bacterium E.coli.1 Publication

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    pH dependencei

    Optimum pH is 8.5.

    GO - Molecular functioni

    1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BRENDAi3.5.1.28. 74.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylmuramoyl-L-alanine amidase A (EC:3.5.1.28)
    Gene namesi
    Name:cwhA
    OrganismiAchromobacter lyticus
    Taxonomic identifieri224 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAchromobacter

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 177177N-acetylmuramoyl-L-alanine amidase APRO_0000079618Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi114 ↔ 1211 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP81717.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi172 – 1754Poly-Gly

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P81717-1 [UniParc]FASTAAdd to Basket

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    AVDFGEAIWN PASSSNYSTA SNQTSAVIMH TMEGSYAGSI SWFQNPSAQV    50
    SAHYLIRKSD GQITQMVREY HQAWHAKNHN YYTIGIEHDG RAADAGNWSA 100
    AMVNASARLT KSICARRGVN CASAWKGPGY DTFHLVPDSV RVKGHGMLSG 150
    NENRYDPGKY FPWSNYYNLI NGGGGNP 177
    Length:177
    Mass (Da):19,395
    Last modified:December 1, 2000 - v1
    Checksum:i040D7F5D806C9C53
    GO

    Mass spectrometryi

    Molecular mass is 19395.9 Da from positions 1 - 177. Determined by MALDI. 1 Publication

    Sequence databases

    PIRiJC7276.

    Cross-referencesi

    Sequence databases

    PIRi JC7276.

    3D structure databases

    ProteinModelPortali P81717.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 3.5.1.28. 74.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, CwhA, from Achromobacter lyticus."
      Li S., Norioka S., Sakiyama F.
      J. Biochem. 127:1033-1039(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, DISULFIDE BOND.
      Strain: M497-1.

    Entry informationi

    Entry nameiCWHA_ACHLY
    AccessioniPrimary (citable) accession number: P81717
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    External Data

    Dasty 3