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P81717

- CWHA_ACHLY

UniProt

P81717 - CWHA_ACHLY

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Protein

N-acetylmuramoyl-L-alanine amidase A

Gene

cwhA

Organism
Achromobacter lyticus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Antibacterial activity against Gram-positive bacteria M.luteus, S.aureus, E.faecalis and P.acidilactici and Gram-negative bacterium E.coli.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

pH dependencei

Optimum pH is 8.5.

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. defense response to bacterium Source: UniProtKB-KW
  3. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BRENDAi3.5.1.28. 74.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase A (EC:3.5.1.28)
Gene namesi
Name:cwhA
OrganismiAchromobacter lyticus
Taxonomic identifieri224 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAchromobacter

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 177177N-acetylmuramoyl-L-alanine amidase APRO_0000079618Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi114 ↔ 1211 Publication

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP81717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi172 – 1754Poly-Gly

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

P81717-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
AVDFGEAIWN PASSSNYSTA SNQTSAVIMH TMEGSYAGSI SWFQNPSAQV
60 70 80 90 100
SAHYLIRKSD GQITQMVREY HQAWHAKNHN YYTIGIEHDG RAADAGNWSA
110 120 130 140 150
AMVNASARLT KSICARRGVN CASAWKGPGY DTFHLVPDSV RVKGHGMLSG
160 170
NENRYDPGKY FPWSNYYNLI NGGGGNP
Length:177
Mass (Da):19,395
Last modified:December 1, 2000 - v1
Checksum:i040D7F5D806C9C53
GO

Mass spectrometryi

Molecular mass is 19395.9 Da from positions 1 - 177. Determined by MALDI. 1 Publication

Sequence databases

PIRiJC7276.

Cross-referencesi

Sequence databases

PIRi JC7276.

3D structure databases

ProteinModelPortali P81717.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 3.5.1.28. 74.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, CwhA, from Achromobacter lyticus."
    Li S., Norioka S., Sakiyama F.
    J. Biochem. 127:1033-1039(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, DISULFIDE BOND.
    Strain: M497-1.

Entry informationi

Entry nameiCWHA_ACHLY
AccessioniPrimary (citable) accession number: P81717
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

External Data

Dasty 3