P81709 (LYSC2_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lysozyme C, spleen isozyme EC=3.2.1.17 Alternative name(s): 1,4-beta-N-acetylmuramidase C
Organism	Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier	9615 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents By similarity.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer By similarity.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 130	130	Lysozyme C, spleen isozyme		PRO_0000208846
Sites
	Active site	35	1	By similarity
	Active site	53	1	By similarity
Amino acid modifications
	Disulfide bond	6 ↔ 128		By similarity
	Disulfide bond	30 ↔ 116		By similarity
	Disulfide bond	65 ↔ 81		By similarity
	Disulfide bond	77 ↔ 95		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81709 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 96C9BA30478D60F6	FASTA	130	14,578
	10 20 30 40 50 60 KIFERCELAR TLKNLGLAGY KGVSLANWVC LAKWESNYNT RATNYNPGSK STDYGIFQIN 70 80 90 100 110 120 SRYWCNDGKT PRAVNACHIS CSALLQDDIT QAVACAKRVV SDPNGIRAWV AWRAHCENRD 130 VSQYVRNCGV

References

[1]

"Sequences of two highly divergent canine type c lysozymes: implications for the evolutionary origins of the lysozyme/alpha-lactalbumin superfamily." Grobler J.A., Rao K.R., Pervaiz S., Brew K. Arch. Biochem. Biophys. 313:360-366(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Spleen.

Cross-references

Sequence databases
PIR	S48642.
3D structure databases
ProteinModelPortal	P81709.
SMR	P81709. Positions 1-130.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Proteomic databases
PaxDb	P81709.
PRIDE	P81709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG85133.
HOGENOM	HOG000037357.
HOVERGEN	HBG052297.
InParanoid	P81709.
OrthoDB	EOG48KRCP.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. IPR023346. Lysozyme-like_dom. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSC2_CANFA

Accession

Primary (citable) accession number: P81709

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program