P81706 (GSTA1_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase A Short name=GST A EC=2.5.1.18 Alternative name(s): Class-alpha GGST A1-1
Organism	Cavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier	10141 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Glutathione S-transferase A		PRO_0000185782
Regions
	Domain	2 – 82	81	GST N-terminal
	Domain	84 – 206	123	GST C-terminal
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Sites
	Binding site	8	1	Glutathione By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylserine

Sequences

	Sequence		Length	Mass (Da)
	P81706 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: ADAF1F5661B88B2F	FASTA	218	25,191
	10 20 30 40 50 60 SGKPVLHYFN VQGRMESIRW LLAAAGVEFE EKLIMCQEDL DKLKNDGLLM FQQVPMVEMD 70 80 90 100 110 120 GMKMVQSRAI LNYIATKYNL YGKDTKERLL IDMYTEGMTD LYELFFKVIL APPEEKDAAK 130 140 150 160 170 180 SLIKDRAKNR FLPAFEKVLK SHGQGYLVGN KLSKADILLT ELLYMVEEFD ASLLANFTLL 190 200 210 QALKTRVSNL PNVKKFLQPG SQRKPFPTQE MFEEMRKF

References

[1]	"Amino acid sequence of glutathione S-transferase a from guinea pig liver." Kamei-Hayashi K., Oshino R., Hara S. J. Biochem. 114:835-841(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: Hartley. Tissue: Liver.
[2]	"Purification and characterization of glutathione S-transferases from guinea pig liver." Oshino R., Kamei K., Nishioka M., Shin M. J. Biochem. 107:105-110(1990) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: Hartley. Tissue: Liver.

Cross-references

Sequence databases
PIR	JX0294.
3D structure databases
ProteinModelPortal	P81706.
SMR	P81706. Positions 3-217.
ModBase	Search...
Protein-protein interaction databases
STRING	10141.ENSCPOP00000021161.
Proteomic databases
PRIDE	P81706.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG266414.
HOVERGEN	HBG053749.
InParanoid	P81706.
OrthoDB	EOG479F80.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11571:SF4. PTHR11571:SF4. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA1_CAVPO

Accession

Primary (citable) accession number: P81706

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program