Reviewed, UniProtKB/Swiss-Prot P81669 (BGAL_PINPS)

Names and origin

Protein names	Recommended name:     Putative beta-galactosidase       Short name=Lactase     EC=3.2.1.23
Organism	Pinus pinaster (Maritime pine)
Taxonomic identifier	71647 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Pinaceae › Pinus › Pinus

Protein attributes

Sequence length	44 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Induction	By water stress.
Miscellaneous	On the 2D-gel the determined pI of this protein (spot N76) is: 5.8, its MW is: 32 kDa.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Biological process	Stress response
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›44	›44	Putative beta-galactosidase		PRO_0000057686
Experimental info
	Non-adjacent residues	15 – 16	2
	Non-adjacent residues	29 – 30	2
	Non-terminal residue	1	1
	Non-terminal residue	44	1

Sequences

	Sequence		Length	Mass (Da)
	P81669-1 [UniParc]. Last modified July 15, 1999. Version 1. Checksum: 30886985FF48C9FA	FASTA	44	4,831
	10 20 30 40 VNIGVNYGML GNSYPTGPET ERHFGLNPDT LHVVRHAQGF HNLA

References

[1]	"Separation and characterization of needle and xylem maritime pine proteins." Costa P., Pionneau C., Bauw G., Dubos C., Bahrman N., Kremer A., Frigerio J.-M., Plomion C. Electrophoresis 20:1098-1108(1999) [PubMed: 10344291] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Needle.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.23. 97638.
Family and domain databases
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_PINPS

Accession

Primary (citable) accession number: P81669

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 15, 1999
Last modified:	November 24, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)