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P81650

- BGAL_PSEHA

UniProt

P81650 - BGAL_PSEHA

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Pseudoalteromonas haloplanktis (Alteromonas haloplanktis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Cofactori

Binds 2 magnesium ions per monomer.1 Publication
Binds 1 sodium ion per monomer.By similarity

Enzyme regulationi

Inhibited by zinc, copper and nickel ions. Activated by 2-mercaptoethanol and inhibited by EDTA in vitro.

Kineticsi

  1. KM=2.4 mM for lactose (at pH 7.5 and at 25 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Temperature dependencei

Optimum temperature is 4 degrees Celsius. It does not grow at temperatures higher than 25 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031SubstrateBy similarity
Metal bindingi201 – 2011SodiumBy similarity
Binding sitei201 – 2011SubstrateBy similarity
Sitei356 – 3561Transition state stabilizerBy similarity
Sitei390 – 3901Transition state stabilizerBy similarity
Metal bindingi415 – 4151Magnesium 1By similarity
Metal bindingi417 – 4171Magnesium 1By similarity
Active sitei460 – 4601Proton donorBy similarity
Metal bindingi460 – 4601Magnesium 1By similarity
Binding sitei460 – 4601SubstrateBy similarity
Active sitei536 – 5361NucleophileBy similarity
Metal bindingi596 – 5961Magnesium 2By similarity
Metal bindingi600 – 6001Sodium; via carbonyl oxygenBy similarity
Metal bindingi603 – 6031SodiumBy similarity
Binding sitei603 – 6031SubstrateBy similarity
Binding sitei1012 – 10121SubstrateBy similarity
Sitei1012 – 10121Important for ensuring that an appropriate proportion of lactose is converted to allolactoseBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Beta-D-galactoside galactohydrolase
Lactase
Gene namesi
Name:lacZ
OrganismiPseudoalteromonas haloplanktis (Alteromonas haloplanktis)
Taxonomic identifieri228 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Biotechnological usei

This cold-adapted beta-galactosidase could be used to hydrolyze lactose in milk and dairy products processed in refrigerated plants. It is in fact superior to the current commercial enzyme from K.marxiamus with respect to lactose removal, especially at low temperatures.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 10391038Beta-galactosidasePRO_0000057656Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP81650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 5394Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81650-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSLQHIINR RDWENPITVQ VNQVKAHSPL NGFKTIEDAR ENTQSQKKSL
60 70 80 90 100
NGQWDFKLFD KPEAVDESLL YEKISKELSG DWQSITVPSN WQLHGFDKPI
110 120 130 140 150
YCNVKYPFAV NPPFVPSDNP TGCYRTEFTI TPEQLTQRNH IIFEGVNSAF
160 170 180 190 200
HLWCNGQWVG YSQDSRLPSE FDLSELLVVG TNRIAVMVIR WSDGSYLEDQ
210 220 230 240 250
DMWWLSGIFR DVNLLTKPQS QIRDVFITPD LDACYRDATL HIKTAINAPN
260 270 280 290 300
NYQVAVQIFD GKTSLCEPKI QSTNNKRVDE KGGWSDVVFQ TIAIRSPKKW
310 320 330 340 350
TAETPYLYRC VVSLLDEQGN TVDVEAYNIG FRKVEMLNGQ LCVNGKPLLI
360 370 380 390 400
RGVNRHEHHP ENGHAVSTAD MIEDIKLMKQ NNFNAVRTAH YPNHPLFYEL
410 420 430 440 450
CDELGLYVVD EANIETHGMF PMGRLASDPL WAGAFMSRYT QMVERDKNHA
460 470 480 490 500
SIIIWSLGNE CGHGANHDAM YGWSKSFDPS RPVQYEGGGA NTTATDIICP
510 520 530 540 550
MYSRVDTDIK DDAVPKYSIK KWLSLPGETR PLILCEYAHA MGNSLGSFDD
560 570 580 590 600
YWQAFREYPR LQGGFIWDWV DQGLSKIDEN GKHYWAYGGD FGDELNDRQF
610 620 630 640 650
CINGLLFPDR TPHPSLFEAK YSQQHLQFTL REQNQNQNQN QYSIDVFSDY
660 670 680 690 700
VFRHTDNEKL VWQLIQNGVC VEQGEMALNI APQSTHTLTI KTKTAFEHGA
710 720 730 740 750
QYYLNLDVAL INDSHFANAN HVMDSEQFKL INSNNLNSKS FASATEKSVI
760 770 780 790 800
SVNETDSHLS IENNTFKLVF NQQSGLIEQW LQDDTQVISS PLVDNFYRAP
810 820 830 840 850
LDNDIGVSEV DNLDPNAWEA RWSRAGIGQW QRTCSSINAV QSSVDVRITC
860 870 880 890 900
VFNYEFNGVL QAQTQWLYTL NNTGTISLNV DVNLNDTLPP MPRIGLSTTI
910 920 930 940 950
NKQSDTKVNW LGLGPFENYP DRKSAARFGY YSLSLNELYT PYIFPTDNGL
960 970 980 990 1000
RSDCQLLSIN NLIVTGAFLF AASEYSQNML TQAKHTNELI ADDCIHVHID
1010 1020 1030
HQHMGVGGDD SWSPSTHKEY LLEQKNYNYS LTLTGGITT
Length:1,039
Mass (Da):118,199
Last modified:January 23, 2007 - v2
Checksum:iD77713F653DE8231
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ131635 Genomic DNA. Translation: CAA10470.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ131635 Genomic DNA. Translation: CAA10470.1 .

3D structure databases

ProteinModelPortali P81650.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cold-adapted beta-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis."
    Hoyoux A., Jennes I., Dubois P., Genicot S., Dubail F., Francois J.M., Baise E., Feller G., Gerday C.
    Appl. Environ. Microbiol. 67:1529-1535(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20, COFACTOR, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: TAE 79.

Entry informationi

Entry nameiBGAL_PSEHA
AccessioniPrimary (citable) accession number: P81650
Secondary accession number(s): Q9ZEM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3