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Protein

Alpha-amylase B

Gene

Amy-d

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116CalciumBy similarity1
Metal bindingi165Calcium; via carbonyl oxygenBy similarity1
Metal bindingi174CalciumBy similarity1
Binding sitei202ChlorideBy similarity1
Active sitei204NucleophileBy similarity1
Metal bindingi208Calcium; via carbonyl oxygenBy similarity1
Active sitei241Proton donorBy similarity1
Binding sitei304ChlorideBy similarity1
Sitei306Transition state stabilizerBy similarity1
Binding sitei343ChlorideBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: UniProtKB
  • alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
  • calcium ion binding Source: UniProtKB

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase B (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy-d
Synonyms:AmyB
ORF Names:CG17876
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000078 Amy-d

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
ChainiPRO_000000136519 – 494Alpha-amylase BAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi153 ↔ 167By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP81641
PRIDEiP81641

Expressioni

Gene expression databases

BgeeiFBgn0000079
ExpressionAtlasiP81641 baseline and differential
GenevisibleiP81641 DM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi62629, 2 interactors
STRINGi7227.FBpp0086155

Structurei

3D structure databases

ProteinModelPortaliP81641
SMRiP81641
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
InParanoidiP81641
KOiK01176
OrthoDBiEOG091G07V6

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL
60 70 80 90 100
GPNGYAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEEQFASMVK
110 120 130 140 150
RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSSKSYP GVPYSSLDFN
160 170 180 190 200
PTCAISNYND ANEVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG
210 220 230 240 250
FRVDAAKHMW PADLAVIYGR LKNLNTDHGF ASGSKAYIVQ EVIDMGGEAI
260 270 280 290 300
SKSEYTGLGA ITEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV
310 320 330 340 350
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF
360 370 380 390 400
TDTDQGPPTT DGHNIASPIF NSDNSCSGGW VCEHRWRQIY NMVAFRNTVG
410 420 430 440 450
SDEIQNWWDN GSNQISFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV
460 470 480 490
ISGSKSGSSC TGKTVTVGSD GRASIYIGSS EDDGVLAIHV NAKL
Length:494
Mass (Da):53,795
Last modified:December 1, 2000 - v3
Checksum:iB477CB031754C298
GO

Polymorphismi

At least 6 electrophoretic isozymes are known: Amy1, Amy2, Amy3, Amy4, Amy5 and Amy6. Strains KO123 expresses Amy1; J87 expresses Amy3; 1420#1, L16 and TN256 express Amy6.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti6Missing in strain: KN-22. 1
Natural varianti11A → S in strain: Berkeley, JP-5, JP-35, JP-55, JP-75, KN-12 and Oregon-R. 1
Natural varianti71S → R in strain: 1420#1, JP-169, JP-186, JP-190, KO123, KN-3, KN-9, KN-10, TN22 and TN256. 1
Natural varianti121D → E in strain: JP-55. 1
Natural varianti121D → G in strain: 1420#1, JP-190, JP-169, JP-186, KO123, TN22 and TN256. 1
Natural varianti121D → N in strain: KN-10, KN-3 and KN-9. 1
Natural varianti138S → T in strain: JP-75, KN-17, KN-21, KN-22 and L16. 1
Natural varianti156S → R in strain: 1420#1, JP-169, JP-186, JP-190, KO123, KN-3, KN-9, KN-10, TN22 and TN256. 1
Natural varianti278D → N in strain: 1420#1, AO168, J87, JP-60, JP-169, JP-186, JP-190, KO123, KN-3, KN-9, KN-10, TN22 and TN256. 1
Natural varianti288T → I in strain: KN-15. 1
Natural varianti398T → A in strain: Berkeley, JP-5, JP-35, JP-55, JP-60, JP-65, JP-70, JP-75, JP-169, JP-186, JP-190, KN-3, KN-9, KN-10, KN-12, KN-17, KN-21 and KN-23. 1
Natural varianti401S → L in strain: Berkeley, JP-5, JP-35, JP-55, JP-65, JP-70, KN-12 and KN-21. 1
Natural varianti403E → A in strain: 1420#1, AO168, J87, JP-60, JP-169, JP-186, JP-190, KO123, KN-3, KN-9, KN-10, TN22 and TN256. 1
Natural varianti410N → S in strain: Berkeley, JP-5, JP-35, JP-55, JP-65 and KN-12. 1
Natural varianti465V → I in strain: JP-1, JP-15, JP-84, KO140 and KN-27. 1
Natural varianti476Y → N in strain: 1420#1, AO168, J87, JP-1, JP-15, JP-55, JP-60, JP-75, JP-84, JP-169, JP-186, JP-190, KO123, KO140, KN-3, KN-9, KN-10, KN-15, KN-17, KN-21, KN-22, KN-23, KN-27, L16, TN22 and TN256. 1
Natural varianti478G → A in strain: KN-21. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84405 Genomic DNA Translation: CAA59126.1
L22717 Genomic DNA Translation: AAA92227.1
L22718 Genomic DNA Translation: AAA92228.1
L22720 Genomic DNA Translation: AAA92230.1
L22724 Genomic DNA Translation: AAA92232.1
L22727 Genomic DNA Translation: AAA92233.1
L22728 Genomic DNA Translation: AAA92242.1
L22730 Genomic DNA Translation: AAA92237.1
L22732 Genomic DNA Translation: AAA92243.1
L22734 Genomic DNA Translation: AAA92238.1
AB043027 Genomic DNA Translation: BAB32525.1
AB043028 Genomic DNA Translation: BAB32526.1
AB043029 Genomic DNA Translation: BAB32527.1
AB043030 Genomic DNA Translation: BAB32528.1
AB043031 Genomic DNA Translation: BAB32529.1
AB043032 Genomic DNA Translation: BAB32530.1
AB043033 Genomic DNA Translation: BAB32531.1
AB043034 Genomic DNA Translation: BAB32532.1
AB043035 Genomic DNA Translation: BAB32533.1
AB043036 Genomic DNA Translation: BAB32534.1
AB043037 Genomic DNA Translation: BAB32535.1
AB043039 Genomic DNA Translation: BAB32537.1
AB043040 Genomic DNA Translation: BAB32538.1
AB043041 Genomic DNA Translation: BAB32539.1
AB043042 Genomic DNA Translation: BAB32540.1
AB043043 Genomic DNA Translation: BAB32541.1
AB043044 Genomic DNA Translation: BAB32542.1
AB043045 Genomic DNA Translation: BAB32543.1
AB043046 Genomic DNA Translation: BAB32544.1
AB043047 Genomic DNA Translation: BAB32545.1
AB043049 Genomic DNA Translation: BAB72141.1
AB043050 Genomic DNA Translation: BAB72142.1
AB043051 Genomic DNA Translation: BAB72143.1
AE013599 Genomic DNA Translation: AAF57894.1
X04570 Genomic DNA Translation: CAA28239.1
X84410 Genomic DNA Translation: CAA59130.1
PIRiS58951
S58954
S58959
S58960
S58962
S58964
RefSeqiNP_523768.1, NM_079044.2
UniGeneiDm.18520

Genome annotation databases

GeneIDi36932
KEGGidme:Dmel_CG17876

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAMYB_DROME
AccessioniPrimary (citable) accession number: P81641
Secondary accession number(s): Q27578
, Q27581, Q27585, Q27882, Q27885, Q27897, Q8WP56, Q95NK4, Q969D1, Q9BH39, Q9BH72, Q9BPS5, Q9BPS6, Q9BPS7, Q9BPS8, Q9BPT0, Q9BPT1, Q9BPT2, Q9BPT3, Q9V7Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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