Reviewed, UniProtKB/Swiss-Prot P81609 (COG2_CARMA)

Names and origin

Protein names	Recommended name:     Collagenolytic protease     EC=3.4.21.32 Alternative name(s):     CSC
Organism	Carcinus maenas (Common shore crab) (Green crab)
Taxonomic identifier	6759 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Brachyura › Eubrachyura › Portunoidea › Portunidae › Carcinus

Protein attributes

Sequence length	17 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serine protease with chymotryptic, collagenolytic and SANA activities. Ref.1
Catalytic activity	Hydrolysis of proteins, with broad specificity for peptide bonds. Native collagen is cleaved about 75% of the length of the molecule from the N-terminus. Low activity on small molecule substrates of both trypsin and chymotrypsin. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Secreted › extracellular space. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
biophysicochemical properties	pH dependence: Optimum pH is 7-8. Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Biological process	Collagen degradation
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›17	›17	Collagenolytic protease		PRO_0000088665
Regions
	Domain	1 – ›17	›17	Peptidase S1
Experimental info
	Non-terminal residue	17	1

Sequences

	Sequence		Length	Mass (Da)
	P81609-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 596E7281B91DA968	FASTA	17	1,817
	10 IVGGMEATPH SWPHQVA

References

[1]

"Purification, kinetical and molecular characterizations of a serine collagenolytic protease from greenshore crab (Carcinus maenas) digestive gland." Roy P., Colas B., Durand P. Comp. Biochem. Physiol. 115B:87-95(1996) [PubMed: 8896334] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Digestive gland.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.32. 19006.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. Partial match. PS00134. TRYPSIN_HIS. Partial match. PS00135. TRYPSIN_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COG2_CARMA

Accession

Primary (citable) accession number: P81609

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)