P81605 (DCD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dermcidin

Alternative name(s):
Preproteolysin

Cleaved into the following 2 chains:

1. Survival-promoting peptide
2. DCD-1

Gene names

Name:	DCD
Synonyms:	AIDD, DSEP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Survival-promoting peptide promotes survival of neurons and displays phosphatase activity. It may bind IgG.
Cofactor	Manganese. Required for survival-promoting peptide.
Subcellular location	Secreted.
Tissue specificity	Specifically and constitutively expressed in eccrine sweat gland cells. Secreted into the sweat at a concentration of 1-10 micrograms/ml.
Mass spectrometry	Molecular mass is 4702.57 Da from positions 63 - 109. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Manganese
Molecular function	Antibiotic Antimicrobial Fungicide
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Ref.5 Ref.6

Chain

20 – 110

91

Dermcidin

PRO_0000021081

Peptide

20 – 49

30

Survival-promoting peptide Ref.5

PRO_0000021082

Propeptide

50 – 62

13

PRO_0000021083

Peptide

63 – 109

47

DCD-1 Ref.1

PRO_0000021084

Propeptide

110

1

PRO_0000408312

Amino acid modifications

Modified residue

68

1

N6-acetyllysine Ref.7

Secondary structure

1

.

.

.

.

.

110

Helix Strand Turn

Details...

Helix

88 – 93

6

Helix

99 – 107

9

Sequences

	Sequence		Length	Mass (Da)
	P81605 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 23666FBD20019465	FASTA	110	11,284
	10 20 30 40 50 60 MRFMTLLFLT ALAGALVCAY DPEAASAPGS GNPCHEASAA QKENAGEDPG LARQAPKPRK 70 80 90 100 110 QRSSLLEKGL DGAKKAVGGL GKLGKDAVED LESVGKGAVH DVKDVLDSVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dermcidin: a novel human antibiotic peptide secreted by sweat glands." Schittek B., Hipfel R., Sauer B., Bauer J., Kalbacher H., Stevanovic S., Schirle M., Schroeder K., Blin N., Meier F., Rassner G., Garbe C. Nat. Immunol. 2:1133-1137(2001) [PubMed: 11694882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 63-109, MASS SPECTROMETRY. Tissue: Melanoma.
[2]	"Overexpression of new survival/evasion peptide (DSEP) attenuates retinoic acid responses and protects cells." Cunningham T.J., Jing H., Akerblom I., Morgan R., Fisher T.S., Neveu M. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Genomic structure and chromosomal mapping of human preproteolysin gene." Tang X.D., Daggett H., Hoshi T. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[5]	"Identification of a survival-promoting peptide in medium conditioned by oxidatively stressed cell lines of nervous system origin." Cunningham T.J., Hodge L., Speicher D., Reim D., Tyler-Polsz C., Levitt P., Eagleson K., Kennedy S., Wang Y. J. Neurosci. 18:7047-7060(1998) [PubMed: 9736629] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-49. Tissue: Retinoblastoma.
[6]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-34.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-68, MASS SPECTROMETRY.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Analysis of the solution structure of the human antibiotic peptide dermcidin and its interaction with phospholipid vesicles." Jung H.H., Yang S.T., Sim J.Y., Lee S., Lee J.Y., Kim H.H., Shin S.Y., Kim J.I. BMB Rep. 43:362-368(2010) [PubMed: 20510021] [Abstract] Cited for: STRUCTURE BY NMR OF 63-110.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Smart sweat - Issue 18 of January 2002

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF144011 mRNA. Translation: AAL18349.1.
AY044239 Genomic DNA. Translation: AAK94785.1.
AF418981 Genomic DNA. Translation: AAL25801.1.
BC062682 mRNA. Translation: AAH62682.1.
BC069108 mRNA. Translation: AAH69108.1.

IPI

IPI00027547.

RefSeq

NP_444513.1. NM_053283.2.

UniGene

Hs.350570.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KSG	NMR	-	A	63-110	[»]

ProteinModelPortal

P81605.

SMR

P81605. Positions 63-110.

ModBase

Search...

Protein-protein interaction databases

IntAct

P81605. 10 interactions.

STRING

P81605.

PTM databases

PhosphoSite

P81605.

Polymorphism databases

DMDM

20141302.

2D gel databases

UCD-2DPAGE

P81605.

Proteomic databases

PeptideAtlas

P81605.

PRIDE

P81605.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000293371; ENSP00000293371; ENSG00000161634.

GeneID

117159.

KEGG

hsa:117159.

UCSC

uc001sgj.1. human.

Organism-specific databases

CTD

117159.

GeneCards

GC12M055038.

H-InvDB

HIX0036710.

HGNC

HGNC:14669. DCD.

MIM

606634. gene.

neXtProt

NX_P81605.

PharmGKB

PA27171.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21190.

HOVERGEN

HBG018946.

InParanoid

P81605.

OrthoDB

EOG4V172H.

PhylomeDB

P81605.

Gene expression databases

ArrayExpress

P81605.

Bgee

P81605.

CleanEx

HS_DCD.

Genevestigator

P81605.

GermOnline

ENSG00000161634. Homo sapiens.

Family and domain databases

ProtoNet

Search...

Other

NextBio

80125.

PMAP-CutDB

P81605.

SOURCE

Search...

Entry information

Entry name

DCD_HUMAN

Accession

Primary (citable) accession number: P81605
Secondary accession number(s): P58461

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 23, 2002
Last modified:	January 25, 2012
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.