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Protein

Dermcidin

Gene

DCD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity.
Survival-promoting peptide promotes survival of neurons and displays phosphatase activity. It may bind IgG.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Mn2+Note: Mn2+ is required for survival-promoting peptide.

GO - Molecular functioni

  • peptidase activity Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide, Hydrolase, Protease

Keywords - Ligandi

Manganese

Protein family/group databases

TCDBi1.C.108.1.1. the pore-forming dermcidin (dermcidin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Dermcidin (EC:3.4.-.-)
Alternative name(s):
Preproteolysin
Cleaved into the following 2 chains:
Gene namesi
Name:DCD
Synonyms:AIDD, DSEP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:14669. DCD.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27171.

Polymorphism and mutation databases

BioMutaiDCD.
DMDMi20141302.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19192 PublicationsAdd
BLAST
Chaini20 – 11091DermcidinPRO_0000021081Add
BLAST
Peptidei20 – 4930Survival-promoting peptidePRO_0000021082Add
BLAST
Propeptidei50 – 62131 PublicationPRO_0000021083Add
BLAST
Peptidei63 – 10947DCD-1PRO_0000021084Add
BLAST
Propeptidei110 – 1101PRO_0000408312

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei68 – 681N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP81605.
PaxDbiP81605.
PeptideAtlasiP81605.
PRIDEiP81605.

2D gel databases

UCD-2DPAGEP81605.

PTM databases

PhosphoSiteiP81605.

Miscellaneous databases

PMAP-CutDBP81605.

Expressioni

Tissue specificityi

Specifically and constitutively expressed in eccrine sweat gland cells. Secreted into the sweat at a concentration of 1-10 micrograms/ml.

Gene expression databases

BgeeiP81605.
CleanExiHS_DCD.
GenevisibleiP81605. HS.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
APPBP2Q926243EBI-395625,EBI-743771

Protein-protein interaction databases

BioGridi125564. 29 interactions.
IntActiP81605. 12 interactions.
MINTiMINT-5000551.
STRINGi9606.ENSP00000293371.

Structurei

Secondary structure

1
110
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi67 – 10741Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KSGNMR-A63-110[»]
2YMKX-ray2.49A/B/C63-110[»]
ProteinModelPortaliP81605.
SMRiP81605. Positions 63-110.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81605.

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG75451.
GeneTreeiENSGT00390000016873.
HOGENOMiHOG000280682.
HOVERGENiHBG018946.
InParanoidiP81605.
OMAiPCHEASA.
OrthoDBiEOG7J9VSJ.
PhylomeDBiP81605.
TreeFamiTF340896.

Family and domain databases

InterProiIPR028130. Dermcidin.
[Graphical view]
PfamiPF15291. Dermcidin. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P81605-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRFMTLLFLT ALAGALVCAY DPEAASAPGS GNPCHEASAA QKENAGEDPG
60 70 80 90 100
LARQAPKPRK QRSSLLEKGL DGAKKAVGGL GKLGKDAVED LESVGKGAVH
110
DVKDVLDSVL
Length:110
Mass (Da):11,284
Last modified:January 23, 2002 - v2
Checksum:i23666FBD20019465
GO
Isoform 2 (identifier: P81605-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-110: AVHDVKDVLDSVL → GEERLVFGAPVNLTSIPLTSVSRP

Show »
Length:121
Mass (Da):12,414
Checksum:i1ED0E009B72ADA55
GO
Isoform 3 (identifier: P81605-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-77: PCHEASAAQK...KGLDGAKKAV → HKQMDCLQLQ...AKPPHTRGLV
     78-110: Missing.

Show »
Length:77
Mass (Da):8,295
Checksum:i60CB579573DA59AA
GO

Mass spectrometryi

Molecular mass is 4702.57 Da from positions 63 - 109. Determined by ESI. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei33 – 7745PCHEA…AKKAV → HKQMDCLQLQKPPSETAKFL SSSTNLPRREKLVPSAKPPH TRGLV in isoform 3. 1 PublicationVSP_043765Add
BLAST
Alternative sequencei78 – 11033Missing in isoform 3. 1 PublicationVSP_043766Add
BLAST
Alternative sequencei98 – 11013AVHDV…LDSVL → GEERLVFGAPVNLTSIPLTS VSRP in isoform 2. CuratedVSP_043767Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144011 mRNA. Translation: AAL18349.1.
AY044239 Genomic DNA. Translation: AAK94785.1.
AF418981 Genomic DNA. Translation: AAL25801.1.
AY590150 mRNA. Translation: AAS99907.1.
EF503687 mRNA. Translation: ABQ53649.1.
EF503688 mRNA. Translation: ABQ53650.1.
EF503689 mRNA. Translation: ABQ53651.1.
AC079310 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96794.1.
BC062682 mRNA. Translation: AAH62682.1.
BC069108 mRNA. Translation: AAH69108.1.
CCDSiCCDS73478.1. [P81605-2]
CCDS8884.1. [P81605-1]
RefSeqiNP_001287783.1. NM_001300854.1. [P81605-2]
NP_444513.1. NM_053283.3. [P81605-1]
UniGeneiHs.350570.

Genome annotation databases

EnsembliENST00000293371; ENSP00000293371; ENSG00000161634.
ENST00000456047; ENSP00000406773; ENSG00000161634. [P81605-2]
ENST00000546807; ENSP00000450415; ENSG00000161634. [P81605-3]
GeneIDi117159.
KEGGihsa:117159.
UCSCiuc001sgj.3. human. [P81605-1]
uc009znt.3. human. [P81605-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Protein Spotlight

Smart sweat - Issue 18 of January 2002

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144011 mRNA. Translation: AAL18349.1.
AY044239 Genomic DNA. Translation: AAK94785.1.
AF418981 Genomic DNA. Translation: AAL25801.1.
AY590150 mRNA. Translation: AAS99907.1.
EF503687 mRNA. Translation: ABQ53649.1.
EF503688 mRNA. Translation: ABQ53650.1.
EF503689 mRNA. Translation: ABQ53651.1.
AC079310 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96794.1.
BC062682 mRNA. Translation: AAH62682.1.
BC069108 mRNA. Translation: AAH69108.1.
CCDSiCCDS73478.1. [P81605-2]
CCDS8884.1. [P81605-1]
RefSeqiNP_001287783.1. NM_001300854.1. [P81605-2]
NP_444513.1. NM_053283.3. [P81605-1]
UniGeneiHs.350570.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KSGNMR-A63-110[»]
2YMKX-ray2.49A/B/C63-110[»]
ProteinModelPortaliP81605.
SMRiP81605. Positions 63-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125564. 29 interactions.
IntActiP81605. 12 interactions.
MINTiMINT-5000551.
STRINGi9606.ENSP00000293371.

Protein family/group databases

TCDBi1.C.108.1.1. the pore-forming dermcidin (dermcidin) family.

PTM databases

PhosphoSiteiP81605.

Polymorphism and mutation databases

BioMutaiDCD.
DMDMi20141302.

2D gel databases

UCD-2DPAGEP81605.

Proteomic databases

MaxQBiP81605.
PaxDbiP81605.
PeptideAtlasiP81605.
PRIDEiP81605.

Protocols and materials databases

DNASUi117159.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000293371; ENSP00000293371; ENSG00000161634.
ENST00000456047; ENSP00000406773; ENSG00000161634. [P81605-2]
ENST00000546807; ENSP00000450415; ENSG00000161634. [P81605-3]
GeneIDi117159.
KEGGihsa:117159.
UCSCiuc001sgj.3. human. [P81605-1]
uc009znt.3. human. [P81605-2]

Organism-specific databases

CTDi117159.
GeneCardsiGC12M055038.
HGNCiHGNC:14669. DCD.
MIMi606634. gene.
neXtProtiNX_P81605.
PharmGKBiPA27171.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG75451.
GeneTreeiENSGT00390000016873.
HOGENOMiHOG000280682.
HOVERGENiHBG018946.
InParanoidiP81605.
OMAiPCHEASA.
OrthoDBiEOG7J9VSJ.
PhylomeDBiP81605.
TreeFamiTF340896.

Miscellaneous databases

EvolutionaryTraceiP81605.
GeneWikiiDermcidin.
GenomeRNAii117159.
NextBioi80125.
PMAP-CutDBP81605.
PROiP81605.
SOURCEiSearch...

Gene expression databases

BgeeiP81605.
CleanExiHS_DCD.
GenevisibleiP81605. HS.

Family and domain databases

InterProiIPR028130. Dermcidin.
[Graphical view]
PfamiPF15291. Dermcidin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 63-109, MASS SPECTROMETRY.
    Tissue: Melanoma.
  2. "Overexpression of new survival/evasion peptide (DSEP) attenuates retinoic acid responses and protects cells."
    Cunningham T.J., Jing H., Akerblom I., Morgan R., Fisher T.S., Neveu M.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Genomic structure and chromosomal mapping of human preproteolysin gene."
    Tang X.D., Daggett H., Hoshi T.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Structural and functional homologies of human proteolysis inducing factor."
    Lowrie A.G., Wigmore S.J., Deans D.A.C., Fearon K.C.H., Waddell I., Ross J.A.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  5. "Identification of dermcidin in human gestational tissue and characterization of its proteolytic activity."
    Lee Motoyama J.P., Kim-Motoyama H., Kim P., Nakagama H., Miyagawa K., Suzuki K.
    Biochem. Biophys. Res. Commun. 357:828-833(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3 AND 3), PROTEOLYTIC FUNCTION, ALTERNATIVE SPLICING.
    Tissue: Placenta.
  6. "The finished DNA sequence of human chromosome 12."
    Baylor College of Medicine Human Genome Sequencing Center Sequence Production Team
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  9. "Identification of a survival-promoting peptide in medium conditioned by oxidatively stressed cell lines of nervous system origin."
    Cunningham T.J., Hodge L., Speicher D., Reim D., Tyler-Polsz C., Levitt P., Eagleson K., Kennedy S., Wang Y.
    J. Neurosci. 18:7047-7060(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-49.
    Tissue: Retinoblastoma.
  10. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-34.
  11. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  14. "Analysis of the solution structure of the human antibiotic peptide dermcidin and its interaction with phospholipid vesicles."
    Jung H.H., Yang S.T., Sim J.Y., Lee S., Lee J.Y., Kim H.H., Shin S.Y., Kim J.I.
    BMB Rep. 43:362-368(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 63-110.

Entry informationi

Entry nameiDCD_HUMAN
AccessioniPrimary (citable) accession number: P81605
Secondary accession number(s): A5JHP2
, A5JHP3, P58461, Q53YJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2002
Last modified: July 22, 2015
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.