P81563 (MMP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interstitial collagenase
EC=3.4.24.7

Alternative name(s):
Fibroblast collagenase
Matrix metalloproteinase-1
Short name=MMP-1
Myocardial collagenase

Gene names

Name:

Mmp1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	15 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. May play a role in the deterioration of the heart wall extracellular matrix proteins during the onset of dilated cardiomyopathy.
Catalytic activity	Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.
Cofactor	Binds 4 calcium ions per subunit By similarity. Binds 2 zinc ions per subunit By similarity.
Enzyme regulation	Can be activated without removal of the activation peptide.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the peptidase M10A family.

Ontologies

Keywords
Biological process	Collagen degradation
Cellular component	Extracellular matrix Secreted
Ligand	Calcium Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›15	›15	Interstitial collagenase		PRO_0000078182
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	15	1

Sequences

	Sequence		Length	Mass (Da)
	P81563 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: 15A57D24C0F6FD80	FASTA	15	1,787
	10 DTLKSEKNAD FKDLY

References

[1]	"Myocardial collagenase: purification and structural characterization." Tyagi S.C., Cleutjens J.P.M. Can. J. Cardiol. 12:165-171(1996) [PubMed: 8605638] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Heart.

Cross-references

Sequence databases
IPI	IPI00202367.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Gene expression databases
Genevestigator	P81563.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MMP1_RAT

Accession

Primary (citable) accession number: P81563

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program