Reviewed,
UniProtKB/Swiss-Prot P81534 (D103A_HUMAN)
Last modified
November 4, 2008.
Version 81.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Beta-defensin 103 Alternative name(s): Defensin, beta 103 Beta-defensin 3 Short name=BD-3 Short name=hBD-3 HBD3 DEFB-3 Defensin-like protein | |||||||
| Gene names |
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| Organism | Homo sapiens (Human) | |||||||
| Taxonomic identifier | 9606 [NCBI] | |||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 67 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed. |
| Subcellular location | |
| Tissue specificity | Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine. |
| Induction | By infection of bacteria and by interferon gamma. |
| Sequence similarities | Belongs to the beta-defensin family. |
| Mass spectrometry | Molecular weight is 5154.59 Da from positions 23 - 67. Determined by ESI. Ref.1 |
Ontologies
Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | Signal |
| Molecular function | Antibiotic Antimicrobial Defensin |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria Ref.1 Traceable author statement. Source: UniProtKB |
| Cellular component | extracellular region Non-traceable author statement. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||
Molecule processing | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 22 | 22 | ||||||||||||||||||
| Peptide | 23 – 67 | 45 | Beta-defensin 103 | PRO_0000006971 | ||||||||||||||||
Amino acid modifications | ||||||||||||||||||||
| Disulfide bond | 33 ↔ 62 | |||||||||||||||||||
| Disulfide bond | 40 ↔ 55 | |||||||||||||||||||
| Disulfide bond | 45 ↔ 63 | |||||||||||||||||||
Experimental info | ||||||||||||||||||||
| Sequence conflict | 45 | 1 | C → R in AAM62424. Ref.6 | |||||||||||||||||
Secondary structure | ||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||
| Turn | 29 – 31 | 3 | ||||||||||||||||||
| Helix | 32 – 36 | 5 | ||||||||||||||||||
| Beta strand | 39 – 44 | 6 | ||||||||||||||||||
| Beta strand | 49 – 53 | 5 | ||||||||||||||||||
| Beta strand | 55 – 59 | 5 | ||||||||||||||||||
| Beta strand | 61 – 65 | 5 | ||||||||||||||||||
Sequences
References
| [1] | "Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic." Harder J., Bartels J., Christophers E., Schroeder J.-M. J. Biol. Chem. 276:5707-5713(2001) [PubMed: 11085990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-67, FUNCTION, TISSUE SPECIFICITY, INDUCTION, MASS SPECTROMETRY. Tissue: Keratinocyte, Lung epithelium and Tracheal epithelium. |
| [2] | "Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction." Conejo-Garcia J.-R., Jaumann F., Schulz S., Krause A., Rodriguez-Jimenez F.-J., Forssmann U., Adermann K., Kluever E., Vogelmeier C., Becker D., Hedrich R., Forssmann W.-G., Bals R. Cell Tissue Res. 306:257-264(2001) [PubMed: 11702237] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. |
| [3] | "Discovery of new human beta-defensins using a genomics-based approach." Jia H.P., Schutte B.C., Schudy A., Linzmeier R., Guthmiller J.M., Johnson G.K., Tack B.F., Mitros J.P., Rosenthal A., Ganz T., McCray P.B. Jr. Gene 263:211-218(2001) [PubMed: 11223260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | Imai Y. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [5] | "EST and genomic database mining yield novel human and mouse beta-defensins." Adler D.A., Diamond G., Sheppard P., Holloway J., Presnell S., Jaspers S., Whitmore T., Fox B., Gosink J., Rixon M., Gao Z., Haldeman B., O'Hara P. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [6] | "Cloning and expression of Chinese human beta defensin-3." Chen S., He F., Li R. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Tonsil. |
| [7] | "The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus." Schibli D.J., Hunter H.N., Aseyev V., Starner T.D., Wiencek J.M., McCray P.B. Jr., Tack B.F., Vogel H.J. J. Biol. Chem. 277:8279-8289(2002) [PubMed: 11741980] [Abstract] Cited for: STRUCTURE BY NMR OF 23-67. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AJ237673 mRNA. Translation: CAC03097.1. AF295370 mRNA. Translation: AAG02237.1. AF217245 mRNA. Translation: AAF73853.1. AB037972 mRNA. Translation: BAB40572.1. AF301470 mRNA. Translation: AAG22030.1. AF516673 mRNA. Translation: AAM62424.1. | |||||||||||||
| RefSeq | NP_001075020.1. NP_061131.1. | ||||||||||||
| UniGene | Hs.283082 Hs.637221 | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000176797. Homo sapiens. [Contig view] ENSG00000177243. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 414325. 55894. | ||||||||||||
| KEGG | hsa:414325. hsa:55894. | ||||||||||||
Organism-specific databases | |||||||||||||
| H-InvDB | HIX0034237. HIX0058445. | ||||||||||||
| HGNC | HGNC:15967. DEFB103A. HGNC:31702. DEFB103B. | ||||||||||||
| MIM | 606611. gene. | ||||||||||||
| PharmGKB | PA27244. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
| GeneCards | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | P81534. | ||||||||||||
| HOVERGEN | P81534. | ||||||||||||
Gene expression databases | |||||||||||||
| CleanEx | HS_DEFB103B. | ||||||||||||
| GermOnline | ENSG00000176797. Homo sapiens. ENSG00000177243. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR001855. Defensin_beta. [Graphical view] | ||||||||||||
| Pfam | PF00711. Defensin_beta. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 108587. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | D103A_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P81534 Secondary accession number(s): Q8NFG6, Q9NPF6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 8 Human chromosome 8: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


