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P81529

- MIFH_TRISP

UniProt

P81529 - MIFH_TRISP

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Protein
Macrophage migration inhibitory factor homolog
Gene
N/A
Organism
Trichinella spiralis (Trichina worm)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Tautomerization of the methyl ester of L-dopachrome. Inhibits migration of human peripheral blood mononuclear cells.2 Publications

Catalytic activityi

L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.2 Publications
Keto-phenylpyruvate = enol-phenylpyruvate.2 Publications

Kineticsi

  1. KM=144 µM for phenylpyruvate1 Publication
  2. KM=783 µM for p-hydroxyphenylpyruvate

Vmax=1026 µmol/min/mg enzyme toward phenylpyruvate

Vmax=34.6 µmol/min/mg enzyme toward p-hydroxyphenylpyruvate

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogen By similarity
Binding sitei33 – 331Substrate By similarity
Binding sitei65 – 651Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. dopachrome isomerase activity Source: UniProtKB-EC
  2. phenylpyruvate tautomerase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor homolog (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
OrganismiTrichinella spiralis (Trichina worm)
Taxonomic identifieri6334 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaEnopleaDorylaimiaTrichocephalidaTrichinellidaeTrichinella

Subcellular locationi

Secreted
Note: Secreted by larvae into the host.1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 114113Macrophage migration inhibitory factor homolog
PRO_0000158074Add
BLAST

Expressioni

Developmental stagei

Expressed in newborn larvae, precyst muscle larvae, postcyst muscle larvae and adult worms.1 Publication

Inductioni

Inhibited by free fatty acids and haematin.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108
Helixi12 – 143
Helixi19 – 3113
Helixi35 – 373
Beta strandi39 – 435
Beta strandi47 – 504
Beta strandi58 – 6710
Helixi70 – 8819
Helixi92 – 943
Beta strandi95 – 1017
Helixi104 – 1063
Beta strandi107 – 1093

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HFOX-ray1.65A/B/C/D/E/F2-114[»]
ProteinModelPortaliP81529.
SMRiP81529. Positions 2-114.

Miscellaneous databases

EvolutionaryTraceiP81529.

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81529-1 [UniParc]FASTAAdd to Basket

« Hide

MPIFTLNTNI KATDVPSDFL SSTSALVGNI LSKPGSYVAV HINTDQQLSF    50
GGSTKPAAFG TLMSIGGIEP SRNRDHSAKL FDHLNKKLGI PKNRMYIHFV 100
NLNGDDVGWN GTTF 114
Length:114
Mass (Da):12,337
Last modified:September 22, 2009 - v2
Checksum:i8B68B33C6309FAF1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551K → N in CAB46354. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ012740 mRNA. Translation: CAB46354.1.
AY050661 mRNA. Translation: AAL12629.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ012740 mRNA. Translation: CAB46354.1 .
AY050661 mRNA. Translation: AAL12629.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HFO X-ray 1.65 A/B/C/D/E/F 2-114 [» ]
ProteinModelPortali P81529.
SMRi P81529. Positions 2-114.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P81529.

Family and domain databases

InterProi IPR001398. Macrophage_inhib_fac.
IPR014347. Tautomerase/MIF_sf.
[Graphical view ]
PANTHERi PTHR11954. PTHR11954. 1 hit.
Pfami PF01187. MIF. 1 hit.
[Graphical view ]
ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55331. SSF55331. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-31; 64-71 AND 104-107, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-114.
    Tissue: Larva.
  2. "Molecular expression and characterization of a homologue of host cytokine macrophage migration inhibitory factor from Trichinella spp."
    Wu Z., Boonmars T., Nagano I., Nakada T., Takahashi Y.
    J. Parasitol. 89:507-515(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: ISS314.
  3. "Rapid purification and characterization of L-dopachrome-methyl-ester tautomerase (macrophage migration inhibitory factor) from Trichinella spiralis, Trichuris muris and Brugia pahangi."
    Pennock J.L., Behnke J.M., Bickle Q.D., Devaney E., Grencis R.K., Isaac R.E., Joshua G.W.P., Selkirk M.E., Zhang Y., Meyer D.J.
    Biochem. J. 335:495-498(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20, FUNCTION, CATALYTIC ACTIVITY, INDUCTION.
    Tissue: Larva.

Entry informationi

Entry nameiMIFH_TRISP
AccessioniPrimary (citable) accession number: P81529
Secondary accession number(s): Q95UI8, Q9Y063
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 22, 2009
Last modified: July 9, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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