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Protein

Macrophage migration inhibitory factor homolog

Gene
N/A
Organism
Trichinella spiralis (Trichina worm)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Tautomerization of the methyl ester of L-dopachrome. Inhibits migration of human peripheral blood mononuclear cells.2 Publications

Catalytic activityi

L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.
Keto-phenylpyruvate = enol-phenylpyruvate.

Kineticsi

  1. KM=144 µM for phenylpyruvate1 Publication
  2. KM=783 µM for p-hydroxyphenylpyruvate1 Publication
  1. Vmax=1026 µmol/min/mg enzyme toward phenylpyruvate1 Publication
  2. Vmax=34.6 µmol/min/mg enzyme toward p-hydroxyphenylpyruvate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
Binding sitei33 – 331SubstrateBy similarity
Binding sitei65 – 651Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor homolog (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
OrganismiTrichinella spiralis (Trichina worm)
Taxonomic identifieri6334 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaEnopleaDorylaimiaTrichocephalidaTrichinellidaeTrichinella

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 114113Macrophage migration inhibitory factor homologPRO_0000158074Add
BLAST

Expressioni

Developmental stagei

Expressed in newborn larvae, precyst muscle larvae, postcyst muscle larvae and adult worms.1 Publication

Inductioni

Inhibited by free fatty acids and haematin.1 Publication

Structurei

Secondary structure

1
114
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108Combined sources
Helixi12 – 143Combined sources
Helixi19 – 3113Combined sources
Helixi35 – 373Combined sources
Beta strandi39 – 435Combined sources
Beta strandi47 – 504Combined sources
Beta strandi58 – 6710Combined sources
Helixi70 – 8819Combined sources
Helixi92 – 943Combined sources
Beta strandi95 – 1017Combined sources
Helixi104 – 1063Combined sources
Beta strandi107 – 1093Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HFOX-ray1.65A/B/C/D/E/F2-114[»]
ProteinModelPortaliP81529.
SMRiP81529. Positions 2-114.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81529.

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIFTLNTNI KATDVPSDFL SSTSALVGNI LSKPGSYVAV HINTDQQLSF
60 70 80 90 100
GGSTKPAAFG TLMSIGGIEP SRNRDHSAKL FDHLNKKLGI PKNRMYIHFV
110
NLNGDDVGWN GTTF
Length:114
Mass (Da):12,337
Last modified:September 22, 2009 - v2
Checksum:i8B68B33C6309FAF1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551K → N in CAB46354 (PubMed:11439086).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012740 mRNA. Translation: CAB46354.1.
AY050661 mRNA. Translation: AAL12629.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012740 mRNA. Translation: CAB46354.1.
AY050661 mRNA. Translation: AAL12629.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HFOX-ray1.65A/B/C/D/E/F2-114[»]
ProteinModelPortaliP81529.
SMRiP81529. Positions 2-114.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP81529.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-31; 64-71 AND 104-107, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-114.
    Tissue: Larva.
  2. "Molecular expression and characterization of a homologue of host cytokine macrophage migration inhibitory factor from Trichinella spp."
    Wu Z., Boonmars T., Nagano I., Nakada T., Takahashi Y.
    J. Parasitol. 89:507-515(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: ISS314.
  3. "Rapid purification and characterization of L-dopachrome-methyl-ester tautomerase (macrophage migration inhibitory factor) from Trichinella spiralis, Trichuris muris and Brugia pahangi."
    Pennock J.L., Behnke J.M., Bickle Q.D., Devaney E., Grencis R.K., Isaac R.E., Joshua G.W.P., Selkirk M.E., Zhang Y., Meyer D.J.
    Biochem. J. 335:495-498(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20, FUNCTION, CATALYTIC ACTIVITY, INDUCTION.
    Tissue: Larva.

Entry informationi

Entry nameiMIFH_TRISP
AccessioniPrimary (citable) accession number: P81529
Secondary accession number(s): Q95UI8, Q9Y063
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 22, 2009
Last modified: May 27, 2015
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.