P81529 (MIFH_TRISP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Macrophage migration inhibitory factor homolog Short name=MIF EC=5.3.2.1 Alternative name(s): L-dopachrome isomerase L-dopachrome tautomerase EC=5.3.3.12 Phenylpyruvate tautomerase
Organism	Trichinella spiralis (Trichina worm)
Taxonomic identifier	6334 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Enoplea › Enoplia › Trichocephalida › Trichinellidae › Trichinella

Protein attributes

Sequence length	114 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tautomerization of the methyl ester of L-dopachrome. Inhibits migration of human peripheral blood mononuclear cells. Ref.1 Ref.3
Catalytic activity	L-dopachrome = 5,6-dihydroxyindole-2-carboxylate. Ref.1 Ref.3 Keto-phenylpyruvate = enol-phenylpyruvate. Ref.1 Ref.3
Subcellular location	Secreted. Note: Secreted by larvae into the host. Ref.1
Developmental stage	Expressed in newborn larvae, precyst muscle larvae, postcyst muscle larvae and adult worms. Ref.2
Induction	Inhibited by free fatty acids and haematin. Ref.3
Sequence similarities	Belongs to the MIF family.
Biophysicochemical properties	Kinetic parameters: KM=144 µM for phenylpyruvate Ref.1 KM=783 µM for p-hydroxyphenylpyruvate Vmax=1026 µmol/min/mg enzyme toward phenylpyruvate Vmax=34.6 µmol/min/mg enzyme toward p-hydroxyphenylpyruvate

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Cytokine Isomerase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	dopachrome isomerase activity Inferred from electronic annotation. Source: EC phenylpyruvate tautomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1 Ref.3

Chain

2 – 114

113

Macrophage migration inhibitory factor homolog

PRO_0000158074

Sites

Active site

2

1

Proton acceptor; via imino nitrogen By similarity

Binding site

33

1

Substrate By similarity

Binding site

65

1

Substrate; via amide nitrogen By similarity

Experimental info

Sequence conflict

55

1

K → N in CAB46354. Ref.1

Secondary structure

1

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114

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Helix

12 – 14

3

Helix

19 – 31

13

Helix

35 – 37

3

Beta strand

39 – 43

5

Beta strand

47 – 50

4

Beta strand

58 – 67

10

Helix

70 – 88

19

Helix

92 – 94

3

Beta strand

95 – 101

7

Helix

104 – 106

3

Beta strand

107 – 109

3

Sequences

	Sequence		Length	Mass (Da)
	P81529 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 8B68B33C6309FAF1	FASTA	114	12,337
	10 20 30 40 50 60 MPIFTLNTNI KATDVPSDFL SSTSALVGNI LSKPGSYVAV HINTDQQLSF GGSTKPAAFG 70 80 90 100 110 TLMSIGGIEP SRNRDHSAKL FDHLNKKLGI PKNRMYIHFV NLNGDDVGWN GTTF

References

[1]	"Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis." Tan T.H.P., Edgerton S.A.V., Kumari R., McAlister M.S.B., Roe S.M., Nagl S., Pearl L.H., Selkirk M.E., Bianco A.E., Totty N.F., Engwerda C., Gray C.A., Meyer D.J., Rowe S.M. Biochem. J. 357:373-383(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-31; 64-71 AND 104-107, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-114. Tissue: Larva.
[2]	"Molecular expression and characterization of a homologue of host cytokine macrophage migration inhibitory factor from Trichinella spp." Wu Z., Boonmars T., Nagano I., Nakada T., Takahashi Y. J. Parasitol. 89:507-515(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Strain: ISS314.
[3]	"Rapid purification and characterization of L-dopachrome-methyl-ester tautomerase (macrophage migration inhibitory factor) from Trichinella spiralis, Trichuris muris and Brugia pahangi." Pennock J.L., Behnke J.M., Bickle Q.D., Devaney E., Grencis R.K., Isaac R.E., Joshua G.W.P., Selkirk M.E., Zhang Y., Meyer D.J. Biochem. J. 335:495-498(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20, FUNCTION, CATALYTIC ACTIVITY, INDUCTION. Tissue: Larva.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ012740 mRNA. Translation: CAB46354.1.
AY050661 mRNA. Translation: AAL12629.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HFO	X-ray	1.65	A/B/C/D/E/F	2-114	[»]

ProteinModelPortal

P81529.

SMR

P81529. Positions 2-114.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001398. Macrophage_inhib_fac.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]

PANTHER

PTHR11954. PTHR11954. 1 hit.

Pfam

PF01187. MIF. 1 hit.
[Graphical view]

ProDom

PD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF55331. SSF55331. 1 hit.

PROSITE

PS01158. MIF. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P81529.

Entry information

Entry name

MIFH_TRISP

Accession

Primary (citable) accession number: P81529
Secondary accession number(s): Q95UI8, Q9Y063

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	September 22, 2009
Last modified:	April 3, 2013
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)