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P81529 (MIFH_TRISP) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Macrophage migration inhibitory factor homolog

Short name=MIF
EC=5.3.2.1
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase
EC=5.3.3.12
Phenylpyruvate tautomerase
OrganismTrichinella spiralis (Trichina worm)
Taxonomic identifier6334 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaEnopleaDorylaimiaTrichocephalidaTrichinellidaeTrichinella

Protein attributes

Sequence length114 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tautomerization of the methyl ester of L-dopachrome. Inhibits migration of human peripheral blood mononuclear cells. Ref.1 Ref.3

Catalytic activity

L-dopachrome = 5,6-dihydroxyindole-2-carboxylate. Ref.1 Ref.3

Keto-phenylpyruvate = enol-phenylpyruvate. Ref.1 Ref.3

Subcellular location

Secreted. Note: Secreted by larvae into the host. Ref.1

Developmental stage

Expressed in newborn larvae, precyst muscle larvae, postcyst muscle larvae and adult worms. Ref.2

Induction

Inhibited by free fatty acids and haematin. Ref.3

Sequence similarities

Belongs to the MIF family.

Biophysicochemical properties

Kinetic parameters:

KM=144 µM for phenylpyruvate Ref.1

KM=783 µM for p-hydroxyphenylpyruvate

Vmax=1026 µmol/min/mg enzyme toward phenylpyruvate

Vmax=34.6 µmol/min/mg enzyme toward p-hydroxyphenylpyruvate

Ontologies

Keywords
   Cellular componentSecreted
   Molecular functionCytokine
Isomerase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiondopachrome isomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phenylpyruvate tautomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.3
Chain2 – 114113Macrophage migration inhibitory factor homolog
PRO_0000158074

Sites

Active site21Proton acceptor; via imino nitrogen By similarity
Binding site331Substrate By similarity
Binding site651Substrate; via amide nitrogen By similarity

Experimental info

Sequence conflict551K → N in CAB46354. Ref.1

Secondary structure

....................... 114
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P81529 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 8B68B33C6309FAF1

FASTA11412,337
        10         20         30         40         50         60 
MPIFTLNTNI KATDVPSDFL SSTSALVGNI LSKPGSYVAV HINTDQQLSF GGSTKPAAFG 

        70         80         90        100        110 
TLMSIGGIEP SRNRDHSAKL FDHLNKKLGI PKNRMYIHFV NLNGDDVGWN GTTF 

« Hide

References

[1]"Macrophage migration inhibitory factor of the parasitic nematode Trichinella spiralis."
Tan T.H.P., Edgerton S.A.V., Kumari R., McAlister M.S.B., Roe S.M., Nagl S., Pearl L.H., Selkirk M.E., Bianco A.E., Totty N.F., Engwerda C., Gray C.A., Meyer D.J., Rowe S.M.
Biochem. J. 357:373-383(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-31; 64-71 AND 104-107, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-114.
Tissue: Larva.
[2]"Molecular expression and characterization of a homologue of host cytokine macrophage migration inhibitory factor from Trichinella spp."
Wu Z., Boonmars T., Nagano I., Nakada T., Takahashi Y.
J. Parasitol. 89:507-515(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: ISS314.
[3]"Rapid purification and characterization of L-dopachrome-methyl-ester tautomerase (macrophage migration inhibitory factor) from Trichinella spiralis, Trichuris muris and Brugia pahangi."
Pennock J.L., Behnke J.M., Bickle Q.D., Devaney E., Grencis R.K., Isaac R.E., Joshua G.W.P., Selkirk M.E., Zhang Y., Meyer D.J.
Biochem. J. 335:495-498(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-20, FUNCTION, CATALYTIC ACTIVITY, INDUCTION.
Tissue: Larva.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ012740 mRNA. Translation: CAB46354.1.
AY050661 mRNA. Translation: AAL12629.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HFOX-ray1.65A/B/C/D/E/F2-114[»]
ProteinModelPortalP81529.
SMRP81529. Positions 2-114.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001398. Macrophage_inhib_fac.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERPTHR11954. PTHR11954. 1 hit.
PfamPF01187. MIF. 1 hit.
[Graphical view]
ProDomPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF55331. SSF55331. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP81529.

Entry information

Entry nameMIFH_TRISP
AccessionPrimary (citable) accession number: P81529
Secondary accession number(s): Q95UI8, Q9Y063
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 22, 2009
Last modified: July 9, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references