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Protein

Macrophage migration inhibitory factor homolog

Gene
N/A
Organism
Trichinella spiralis (Trichina worm)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Tautomerization of the methyl ester of L-dopachrome. Inhibits migration of human peripheral blood mononuclear cells.2 Publications

Catalytic activityi

L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.
Keto-phenylpyruvate = enol-phenylpyruvate.

Kineticsi

  1. KM=144 µM for phenylpyruvate1 Publication
  2. KM=783 µM for p-hydroxyphenylpyruvate1 Publication
  1. Vmax=1026 µmol/min/mg enzyme toward phenylpyruvate1 Publication
  2. Vmax=34.6 µmol/min/mg enzyme toward p-hydroxyphenylpyruvate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Proton acceptor; via imino nitrogenBy similarity1
Binding sitei33SubstrateBy similarity1
Binding sitei65Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor homolog (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
OrganismiTrichinella spiralis (Trichina worm)
Taxonomic identifieri6334 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaEnopleaDorylaimiaTrichocephalidaTrichinellidaeTrichinella

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001580742 – 114Macrophage migration inhibitory factor homologAdd BLAST113

Expressioni

Developmental stagei

Expressed in newborn larvae, precyst muscle larvae, postcyst muscle larvae and adult worms.1 Publication

Inductioni

Inhibited by free fatty acids and haematin.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6334.EFV56343.

Structurei

Secondary structure

1114
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Helixi12 – 14Combined sources3
Helixi19 – 31Combined sources13
Helixi35 – 37Combined sources3
Beta strandi39 – 43Combined sources5
Beta strandi47 – 50Combined sources4
Beta strandi58 – 67Combined sources10
Helixi70 – 88Combined sources19
Helixi92 – 94Combined sources3
Beta strandi95 – 101Combined sources7
Helixi104 – 106Combined sources3
Beta strandi107 – 109Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HFOX-ray1.65A/B/C/D/E/F2-114[»]
ProteinModelPortaliP81529.
SMRiP81529.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP81529.

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiKOG1759. Eukaryota.
ENOG41122MF. LUCA.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P81529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIFTLNTNI KATDVPSDFL SSTSALVGNI LSKPGSYVAV HINTDQQLSF
60 70 80 90 100
GGSTKPAAFG TLMSIGGIEP SRNRDHSAKL FDHLNKKLGI PKNRMYIHFV
110
NLNGDDVGWN GTTF
Length:114
Mass (Da):12,337
Last modified:September 22, 2009 - v2
Checksum:i8B68B33C6309FAF1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55K → N in CAB46354 (PubMed:11439086).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012740 mRNA. Translation: CAB46354.1.
AY050661 mRNA. Translation: AAL12629.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012740 mRNA. Translation: CAB46354.1.
AY050661 mRNA. Translation: AAL12629.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HFOX-ray1.65A/B/C/D/E/F2-114[»]
ProteinModelPortaliP81529.
SMRiP81529.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6334.EFV56343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1759. Eukaryota.
ENOG41122MF. LUCA.

Miscellaneous databases

EvolutionaryTraceiP81529.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIFH_TRISP
AccessioniPrimary (citable) accession number: P81529
Secondary accession number(s): Q95UI8, Q9Y063
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 22, 2009
Last modified: November 2, 2016
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.