Reviewed, UniProtKB/Swiss-Prot P81497 (PEPA_SUNMU)

Names and origin

Protein names

Recommended name:
    Pepsin A
    EC=3.4.23.1

Gene names

Name:	PGA
Synonyms:	PGNA

Organism

Suncus murinus (House shrew) (Musk shrew)

Taxonomic identifier

9378 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Insectivora › Soricidae › Crocidurinae › Suncus

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Catalytic activity	Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Phosphoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.2
	Propeptide	17 – 61	45	Activation peptide By similarity		PRO_0000026042
	Chain	62 – 387	326	Pepsin A		PRO_0000026043
Sites
	Active site	93	1	By similarity
	Active site	276	1	By similarity
Amino acid modifications
	Modified residue	129	1	Phosphoserine By similarity
	Disulfide bond	106 ↔ 111		By similarity
	Disulfide bond	267 ↔ 271		By similarity
	Disulfide bond	310 ↔ 343		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P81497-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 7F9FF818DD8541CE	FASTA	387	41,576
	10 20 30 40 50 60 MKKLLLLLGL VALSECLYKV PLVKKKSLRQ NLIENGLLKD FLAKHNVNPA SKYFPTEAAT 70 80 90 100 110 120 ELADQPLVNY MDMEYFGTIG IGTPPQEFTV IFDTGSSNLW VPSVYCSSPA CSNHNRFNPQ 130 140 150 160 170 180 KSSTFQSTSQ TLSIAYGTGS MTGVLGYDTV QVAGIADTNQ IFGLSQTEPG SFLYYSPFDG 190 200 210 220 230 240 ILGLAYPNIA SSGATPVFDN MWNQGLVSQD LFSVYLSSND QSGSVVIFGG IDSSYYTGNL 250 260 270 280 290 300 NWVPLSSEGY WQITVDSITM NGQAIACSGS CQAIVDTGTS LLSGPNNAIA NIQKSIGASQ 310 320 330 340 350 360 NANGQMVVSC SSIQSLPDIV FTINGIQYPL PASAYILQNQ QDCTSGFQGM DIPTPSGELW 370 380 ILGDVFIRQY FAVFDRGNNR VGLAPVA

References

[1]	"Phylogenetic position of Eulipotyphla inferred from the cDNA sequences of pepsinogens A and C." Narita Y., Oda S., Takenaka O., Kageyama T. Mol. Phylogenet. Evol. 21:32-42(2001) [PubMed: 11603935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Pepsinogens and pepsins from house musk shrew, Suncus murinus: purification, characterization, determination of the amino-acid sequences of the activation segments, and analysis of proteolytic specificities." Narita Y., Oda S., Moriyama A., Takenaka O., Kageyama T. J. Biochem. 121:1010-1017(1997) [PubMed: 9354369] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-82. Tissue: Gastric mucosa.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB047243 mRNA. Translation: BAB11749.1.
PIR	PC4360.
3D structure databases
HSSP	HSSP built from PDB template 1PSN based on UniProtKB P00790.
SMR	P81497. Positions 17-387.
ModBase	Search...
Protein family/group databases
MEROPS	A01.001.
Phylogenomic databases
HOVERGEN	P81497.
Enzyme and pathway databases
BRENDA	3.4.23.1. 39436.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPA_SUNMU

Accession

Primary (citable) accession number: P81497
Secondary accession number(s): Q9GMY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)