P81460 (CONA_CANLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Concanavalin-A Short name=Con A
Organism	Canavalia lineata (Beach bean) (Dolichos lineatus)
Taxonomic identifier	28957 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Canavalia

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glucose/D-mannose specific lectin.
Subunit structure	Homotetramer.
Miscellaneous	Binds one manganese (or other transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities.
Sequence similarities	Belongs to the leguminous lectin family.

Ontologies

Keywords
Ligand	Calcium Lectin Manganese Mannose-binding Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	mannose binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 237

237

Concanavalin-A

PRO_0000105087

Regions

Region

99 – 100

2

Carbohydrate binding By similarity

Sites

Metal binding

8

1

Manganese By similarity

Metal binding

10

1

Calcium By similarity

Metal binding

10

1

Manganese By similarity

Metal binding

12

1

Calcium; via carbonyl oxygen By similarity

Metal binding

14

1

Calcium By similarity

Metal binding

19

1

Calcium By similarity

Metal binding

19

1

Manganese By similarity

Metal binding

24

1

Manganese By similarity

Metal binding

208

1

Calcium By similarity

Binding site

12

1

Carbohydrate By similarity

Binding site

228

1

Carbohydrate; via amide nitrogen By similarity

Secondary structure

1

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237

Helix Strand Turn

Details...

Beta strand

4 – 10

7

Helix

15 – 17

3

Beta strand

24 – 33

10

Beta strand

35 – 39

5

Beta strand

46 – 55

10

Turn

56 – 59

4

Beta strand

60 – 66

7

Beta strand

72 – 78

7

Helix

81 – 83

3

Beta strand

87 – 96

10

Beta strand

98 – 100

3

Beta strand

105 – 116

12

Beta strand

124 – 132

9

Beta strand

140 – 144

5

Beta strand

150 – 152

3

Beta strand

154 – 157

4

Turn

161 – 163

3

Beta strand

170 – 177

8

Beta strand

187 – 199

13

Beta strand

203 – 205

3

Beta strand

209 – 216

8

Helix

227 – 229

3

Turn

230 – 232

3

Sequences

	Sequence		Length	Mass (Da)
	P81460 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: 2D31C2BD3D30D66A	FASTA	237	25,497
	10 20 30 40 50 60 ADTIVAVELD TYPNTDIGDP SYPHIGIDIK SVRSKKTAKW NMQNGKVGTA HIIYNSVGKR 70 80 90 100 110 120 LSAVVSYPNG DSATVSYDVD LDNVLPEWVR VGLSASTGLY KETNTILSWS FTSKLKSNST 130 140 150 160 170 180 HETNALHFVF NQFSKDQKDL ILQGDATTGT DGNLELTRVS SNGSPQGNSV GRALFYAPVH 190 200 210 220 230 IWESSAVVAS FDATFTFLIK SSDSHPADGI AFFISNIDSS IPSGSTGRLL GLFPDAN

References

[1]	"Primary structures of concanavalin A-like lectins from seeds of two species of Canavalia." Fujimura S., Terada S., Jayavardhanan K.K., Panikkar K.R., Kimoto E. Phytochemistry 33:985-987(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

A59415.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CWM	X-ray	1.95	A	1-237	[»]
2CY6	X-ray	2.00	A/D	1-237	[»]
2CYF	X-ray	1.80	A	1-237	[»]
3SNM	X-ray	2.15	A	1-237	[»]
4DPN	X-ray	2.55	A/D	1-237	[»]
4I30	X-ray	1.89	A	1-237	[»]

ProteinModelPortal

P81460.

SMR

P81460. Positions 1-237.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.120.200. 2 hits.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]

Pfam

PF00139. Lectin_legB. 2 hits.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

PROSITE

PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P81460.

Entry information

Entry name

CONA_CANLI

Accession

Primary (citable) accession number: P81460

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program