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P81453

- TGAS_STRMB

UniProt

P81453 - TGAS_STRMB

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Protein
Protein-glutamine gamma-glutamyltransferase
Gene
N/A
Organism
Streptomyces mobaraensis (Streptoverticillium mobaraense)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei140 – 1401
Active sitei331 – 3311
Active sitei350 – 3501

GO - Molecular functioni

  1. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-glutamine gamma-glutamyltransferase (EC:2.3.2.13)
    Alternative name(s):
    MTG
    Transglutaminase
    Short name:
    TGase
    OrganismiStreptomyces mobaraensis (Streptoverticillium mobaraense)
    Taxonomic identifieri35621 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Pathology & Biotechi

    Biotechnological usei

    Sold under the name Activa TG by Ajinomoto. It has the ability to cross-link protein molecules present in food without the use of salt or binders. Used to improve some of the physical properties such as firmness, elasticity and moisture retention of food such as meat, poultry and seafood.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131 Reviewed prediction
    Add
    BLAST
    Propeptidei32 – 7645
    PRO_0000033656Add
    BLAST
    Chaini77 – 407331Protein-glutamine gamma-glutamyltransferase
    PRO_0000033657Add
    BLAST

    Keywords - PTMi

    Zymogen

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi42 – 465
    Helixi50 – 6112
    Beta strandi102 – 1065
    Helixi107 – 11610
    Turni117 – 1193
    Helixi130 – 1367
    Helixi142 – 1487
    Beta strandi156 – 1583
    Helixi163 – 17210
    Helixi181 – 19111
    Helixi195 – 21319
    Helixi219 – 23214
    Helixi236 – 2394
    Beta strandi242 – 2443
    Helixi245 – 2484
    Turni249 – 2524
    Helixi254 – 2574
    Turni259 – 2646
    Helixi266 – 2683
    Beta strandi269 – 27810
    Helixi288 – 2936
    Turni296 – 2994
    Turni303 – 3053
    Beta strandi321 – 3244
    Beta strandi331 – 3366
    Helixi343 – 3453
    Beta strandi347 – 3537
    Beta strandi359 – 3635
    Beta strandi365 – 3706
    Helixi371 – 3755
    Beta strandi382 – 39211
    Beta strandi404 – 4063

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IU4X-ray2.40A/B/C/D77-407[»]
    3IU0X-ray1.90A32-407[»]
    ProteinModelPortaliP81453.
    SMRiP81453. Positions 77-407.

    Miscellaneous databases

    EvolutionaryTraceiP81453.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the bacterial TGase family.

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.90.1360.10. 1 hit.
    InterProiIPR015107. Transglut_prok.
    [Graphical view]
    PfamiPF09017. Transglut_prok. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037210. Transglut_prok. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P81453-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIRRRALVF ATMSAVLCTA GFMPSAGEAA ADNGAGEETK SYAETYRLTA    50
    DDVANINALN ESAPAASSAG PSFRAPDSDD RVTPPAEPLD RMPDPYRPSY 100
    GRAETVVNNY IRKWQQVYSH RDGRKQQMTE EQREWLSYGC VGVTWVNSGQ 150
    YPTNRLAFAS FDEDRFKNEL KNGRPRSGET RAEFEGRVAK ESFDEEKGFQ 200
    RAREVASVMN RALENAHDES AYLDNLKKEL ANGNDALRNE DARSPFYSAL 250
    RNTPSFKERN GGNHDPSRMK AVIYSKHFWS GQDRSSSADK RKYGDPDAFR 300
    PAPGTGLVDM SRDRNIPRSP TSPGEGFVNF DYGWFGAQTE ADADKTVWTH 350
    GNHYHAPNGS LGAMHVYESK FRNWSEGYSD FDRGAYVITF IPKSWNTAPD 400
    KVKQGWP 407
    Length:407
    Mass (Da):45,684
    Last modified:November 7, 2003 - v2
    Checksum:i10F7F7A04EAB2DF4
    GO

    Mass spectrometryi

    Molecular mass is 37869.2±8.8 Da from positions 77 - 407. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF531437 Genomic DNA. Translation: AAM95951.1.
    HF968462 Genomic DNA. Translation: CCW72544.1.
    Y18315 Genomic DNA. Translation: CAA77128.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF531437 Genomic DNA. Translation: AAM95951.1 .
    HF968462 Genomic DNA. Translation: CCW72544.1 .
    Y18315 Genomic DNA. Translation: CAA77128.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IU4 X-ray 2.40 A/B/C/D 77-407 [» ]
    3IU0 X-ray 1.90 A 32-407 [» ]
    ProteinModelPortali P81453.
    SMRi P81453. Positions 77-407.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P81453.

    Family and domain databases

    Gene3Di 3.90.1360.10. 1 hit.
    InterProi IPR015107. Transglut_prok.
    [Graphical view ]
    Pfami PF09017. Transglut_prok. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037210. Transglut_prok. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Secretion of active-form Streptoverticillium mobaraense transglutaminase by Corynebacterium glutamicum: processing of the pro-transglutaminase by a cosecreted subtilisin-like protease from Streptomyces albogriseolus."
      Kikuchi Y., Date M., Yokoyama K., Umezawa Y., Matsui H.
      Appl. Environ. Microbiol. 69:358-366(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 29032 / CBS 199.75 / DSM 40847 / NBRC 13819 / NCIMB 11159 / NRRL B-3729 / VKM Ac-928.
    2. "Confirmation of the genes coding a transglutaminase and a prolyl tri/tetrapeptidyl aminopeptidase from Streptomyces mobaraensis."
      Zindel S., Froels S., Kletzin A., Pfeifer F., Fuchsbauer H.L.
      Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 29032 / CBS 199.75 / DSM 40847 / NBRC 13819 / NCIMB 11159 / NRRL B-3729 / VKM Ac-928.
    3. "Bacterial pro-transglutaminase from Streptoverticillium mobaraense: purification, characterisation and sequence of the zymogen."
      Pasternack R., Dorsch S., Otterbach J.T., Robenek I.R., Wolf S., Fuchsbauer H.-L.
      Eur. J. Biochem. 257:570-576(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-407, PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 27441 / CBS 777.72 / DSM 40587 / JCM 4778 / NBRC 13476 / VKM Ac-879.
    4. "Primary structure of microbial transglutaminase from Streptoverticillium sp. strain s-8112."
      Kanaji T., Ozaki H., Takao T., Kawajiri H., Ide H., Motoki M., Shimonishi Y.
      J. Biol. Chem. 268:11565-11572(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 77-407, MASS SPECTROMETRY.
      Strain: S-8112.
    5. "Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense."
      Kashiwagi T., Yokoyama K., Ishikawa K., Ono K., Ejima D., Matsui H., Suzuki E.
      J. Biol. Chem. 277:44252-44260(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 77-407.
      Strain: ATCC 29032 / CBS 199.75 / DSM 40847 / NBRC 13819 / NCIMB 11159 / NRRL B-3729 / VKM Ac-928.

    Entry informationi

    Entry nameiTGAS_STRMB
    AccessioniPrimary (citable) accession number: P81453
    Secondary accession number(s): N1NTU7, Q8KRJ2, Q9ZAF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 7, 2003
    Last modified: April 16, 2014
    This is version 69 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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