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P81453 (TGAS_STRMB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein-glutamine gamma-glutamyltransferase

EC=2.3.2.13
Alternative name(s):
MTG
Transglutaminase
Short name=TGase
OrganismStreptomyces mobaraensis (Streptoverticillium mobaraense)
Taxonomic identifier35621 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.

Catalytic activity

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.

Biotechnological use

Sold under the name Activa TG by Ajinomoto. It has the ability to cross-link protein molecules present in food without the use of salt or binders. Used to improve some of the physical properties such as firmness, elasticity and moisture retention of food such as meat, poultry and seafood.

Sequence similarities

Belongs to the bacterial TGase family.

Mass spectrometry

Molecular mass is 37869.2±8.8 Da from positions 77 - 407. Determined by ESI. Ref.4

Ontologies

Keywords
   DomainSignal
   Molecular functionAcyltransferase
Transferase
   PTMZymogen
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Molecular_functionprotein-glutamine gamma-glutamyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Propeptide32 – 7645
PRO_0000033656
Chain77 – 407331Protein-glutamine gamma-glutamyltransferase
PRO_0000033657

Sites

Active site1401
Active site3311
Active site3501

Secondary structure

........................................................... 407
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P81453 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: 10F7F7A04EAB2DF4

FASTA40745,684
        10         20         30         40         50         60 
MRIRRRALVF ATMSAVLCTA GFMPSAGEAA ADNGAGEETK SYAETYRLTA DDVANINALN 

        70         80         90        100        110        120 
ESAPAASSAG PSFRAPDSDD RVTPPAEPLD RMPDPYRPSY GRAETVVNNY IRKWQQVYSH 

       130        140        150        160        170        180 
RDGRKQQMTE EQREWLSYGC VGVTWVNSGQ YPTNRLAFAS FDEDRFKNEL KNGRPRSGET 

       190        200        210        220        230        240 
RAEFEGRVAK ESFDEEKGFQ RAREVASVMN RALENAHDES AYLDNLKKEL ANGNDALRNE 

       250        260        270        280        290        300 
DARSPFYSAL RNTPSFKERN GGNHDPSRMK AVIYSKHFWS GQDRSSSADK RKYGDPDAFR 

       310        320        330        340        350        360 
PAPGTGLVDM SRDRNIPRSP TSPGEGFVNF DYGWFGAQTE ADADKTVWTH GNHYHAPNGS 

       370        380        390        400 
LGAMHVYESK FRNWSEGYSD FDRGAYVITF IPKSWNTAPD KVKQGWP 

« Hide

References

[1]"Secretion of active-form Streptoverticillium mobaraense transglutaminase by Corynebacterium glutamicum: processing of the pro-transglutaminase by a cosecreted subtilisin-like protease from Streptomyces albogriseolus."
Kikuchi Y., Date M., Yokoyama K., Umezawa Y., Matsui H.
Appl. Environ. Microbiol. 69:358-366(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29032 / CBS 199.75 / DSM 40847 / NBRC 13819 / NCIMB 11159 / NRRL B-3729 / VKM Ac-928.
[2]"Confirmation of the genes coding a transglutaminase and a prolyl tri/tetrapeptidyl aminopeptidase from Streptomyces mobaraensis."
Zindel S., Froels S., Kletzin A., Pfeifer F., Fuchsbauer H.L.
Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29032 / CBS 199.75 / DSM 40847 / NBRC 13819 / NCIMB 11159 / NRRL B-3729 / VKM Ac-928.
[3]"Bacterial pro-transglutaminase from Streptoverticillium mobaraense: purification, characterisation and sequence of the zymogen."
Pasternack R., Dorsch S., Otterbach J.T., Robenek I.R., Wolf S., Fuchsbauer H.-L.
Eur. J. Biochem. 257:570-576(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-407, PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 27441 / CBS 777.72 / DSM 40587 / JCM 4778 / NBRC 13476 / VKM Ac-879.
[4]"Primary structure of microbial transglutaminase from Streptoverticillium sp. strain s-8112."
Kanaji T., Ozaki H., Takao T., Kawajiri H., Ide H., Motoki M., Shimonishi Y.
J. Biol. Chem. 268:11565-11572(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 77-407, MASS SPECTROMETRY.
Strain: S-8112.
[5]"Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense."
Kashiwagi T., Yokoyama K., Ishikawa K., Ono K., Ejima D., Matsui H., Suzuki E.
J. Biol. Chem. 277:44252-44260(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 77-407.
Strain: ATCC 29032 / CBS 199.75 / DSM 40847 / NBRC 13819 / NCIMB 11159 / NRRL B-3729 / VKM Ac-928.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF531437 Genomic DNA. Translation: AAM95951.1.
HF968462 Genomic DNA. Translation: CCW72544.1.
Y18315 Genomic DNA. Translation: CAA77128.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IU4X-ray2.40A/B/C/D77-407[»]
3IU0X-ray1.90A32-407[»]
ProteinModelPortalP81453.
SMRP81453. Positions 77-407.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.90.1360.10. 1 hit.
InterProIPR015107. Transglut_prok.
[Graphical view]
PfamPF09017. Transglut_prok. 1 hit.
[Graphical view]
PIRSFPIRSF037210. Transglut_prok. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP81453.

Entry information

Entry nameTGAS_STRMB
AccessionPrimary (citable) accession number: P81453
Secondary accession number(s): N1NTU7, Q8KRJ2, Q9ZAF5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 7, 2003
Last modified: April 16, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references