Reviewed, UniProtKB/Swiss-Prot P81453 (TGAS_STRMB)

Names and origin

Protein names	Recommended name:     Protein-glutamine gamma-glutamyltransferase     EC=2.3.2.13 Alternative name(s):     Transglutaminase       Short name=TGase     MTG
Organism	Streptomyces mobaraensis (Streptoverticillium mobaraense)
Taxonomic identifier	35621 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH(3).
Biotechnological use	Sold under the name Activa TG by Ajinomoto. It has the ability to cross-link protein molecules present in food without the use of salt or binders. Used to improve some of the physical properties such as firmness, elasticity and moisture retention of food such as meat, poultry and seafood.
Sequence similarities	Belongs to the bacterial TGase family.
Mass spectrometry	Molecular weight is 37869.2±8.8 Da from positions 77 - 407. Determined by ESI. Ref.3

Ontologies

Keywords
Domain	Signal
Molecular function	Acyltransferase Transferase
PTM	Zymogen
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 31

31

Potential

Propeptide

32 – 76

45

PRO_0000033656

Chain

77 – 407

331

Protein-glutamine gamma-glutamyltransferase

PRO_0000033657

Sites

Active site

140

1

Active site

331

1

Active site

350

1

Secondary structure

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407

Helix Strand Turn

Details...

Beta strand

102 – 106

5

Helix

107 – 117

11

Helix

130 – 136

7

Helix

142 – 148

7

Beta strand

156 – 158

3

Helix

163 – 172

10

Helix

181 – 192

12

Helix

195 – 211

17

Turn

212 – 215

4

Helix

219 – 232

14

Helix

236 – 239

4

Beta strand

242 – 244

3

Turn

245 – 248

4

Helix

249 – 252

4

Turn

254 – 257

4

Beta strand

259 – 261

3

Helix

266 – 268

3

Beta strand

269 – 278

10

Helix

288 – 293

6

Turn

296 – 299

4

Turn

303 – 305

3

Beta strand

321 – 324

4

Beta strand

329 – 336

8

Helix

343 – 345

3

Beta strand

347 – 353

7

Beta strand

359 – 363

5

Beta strand

365 – 370

6

Helix

371 – 374

4

Beta strand

383 – 392

10

Sequences

	Sequence		Length	Mass (Da)
	P81453-1 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 10F7F7A04EAB2DF4	FASTA	407	45,684
	10 20 30 40 50 60 MRIRRRALVF ATMSAVLCTA GFMPSAGEAA ADNGAGEETK SYAETYRLTA DDVANINALN 70 80 90 100 110 120 ESAPAASSAG PSFRAPDSDD RVTPPAEPLD RMPDPYRPSY GRAETVVNNY IRKWQQVYSH 130 140 150 160 170 180 RDGRKQQMTE EQREWLSYGC VGVTWVNSGQ YPTNRLAFAS FDEDRFKNEL KNGRPRSGET 190 200 210 220 230 240 RAEFEGRVAK ESFDEEKGFQ RAREVASVMN RALENAHDES AYLDNLKKEL ANGNDALRNE 250 260 270 280 290 300 DARSPFYSAL RNTPSFKERN GGNHDPSRMK AVIYSKHFWS GQDRSSSADK RKYGDPDAFR 310 320 330 340 350 360 PAPGTGLVDM SRDRNIPRSP TSPGEGFVNF DYGWFGAQTE ADADKTVWTH GNHYHAPNGS 370 380 390 400 LGAMHVYESK FRNWSEGYSD FDRGAYVITF IPKSWNTAPD KVKQGWP

References

[1]	"Secretion of active-form Streptoverticillium mobaraense transglutaminase by Corynebacterium glutamicum: processing of the pro-transglutaminase by a cosecreted subtilisin-like protease from Streptomyces albogriseolus." Kikuchi Y., Date M., Yokoyama K., Umezawa Y., Matsui H. Appl. Environ. Microbiol. 69:358-366(2003) [PubMed: 12514016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29032 / CBS 199.75 / DSM 40847 / IFO 13819 / NCIMB 11159.
[2]	"Bacterial pro-transglutaminase from Streptoverticillium mobaraense: purification, characterisation and sequence of the zymogen." Pasternack R., Dorsch S., Otterbach J.T., Robenek I.R., Wolf S., Fuchsbauer H.-L. Eur. J. Biochem. 257:570-576(1998) [PubMed: 9839945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-407, PARTIAL PROTEIN SEQUENCE. Strain: ATCC 27441 / CBS 777.72 / DSM 40587 / IFO 13476 / JCM 4778.
[3]	"Primary structure of microbial transglutaminase from Streptoverticillium sp. strain s-8112." Kanaji T., Ozaki H., Takao T., Kawajiri H., Ide H., Motoki M., Shimonishi Y. J. Biol. Chem. 268:11565-11572(1993) [PubMed: 8099353] [Abstract] Cited for: PROTEIN SEQUENCE OF 77-407, MASS SPECTROMETRY. Strain: S-8112.
[4]	"Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense." Kashiwagi T., Yokoyama K., Ishikawa K., Ono K., Ejima D., Matsui H., Suzuki E. J. Biol. Chem. 277:44252-44260(2002) [PubMed: 12221081] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 77-407. Strain: ATCC 29032 / CBS 199.75 / DSM 40847 / IFO 13819 / NCIMB 11159.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF531437 Genomic DNA. Translation: AAM95951.1.
Y18315 Genomic DNA. Translation: CAA77128.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IU4	X-ray	2.40	A/B/C/D	77-407	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR015107. Transglut_prok.
[Graphical view]

Pfam

PF09017. Transglut_prok. 1 hit.
[Graphical view]

PIRSF

PIRSF037210. Transglut_prok. 1 hit.

ProtoNet

Search...

Entry information

Entry name

TGAS_STRMB

Accession

Primary (citable) accession number: P81453
Secondary accession number(s): Q8KRJ2, Q9ZAF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 7, 2003
Last modified:	November 4, 2008
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)