Reviewed, UniProtKB/Swiss-Prot P81450 (ATP18_YEAST)

Names and origin

Protein names

Recommended name:
    ATP synthase subunit J, mitochondrial
Alternative name(s):
    ATPase synthase I subunit

Gene names

Name:	ATP18
Ordered Locus Names:	YML081C-A
ORF Names:	YML081BC

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	59 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.
Subcellular location	Mitochondrion membrane; Single-pass membrane protein Potential.
Miscellaneous	Present with 6540 molecules/cell in log phase SD medium. Ref.5
Mass spectrometry	Molecular mass is 6687.1±2 Da from positions 1 - 59. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion
Domain	Transmembrane
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP synthesis coupled proton transport Ref.1 Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) Ref.4 Inferred from mutant phenotype. Source: SGD
Molecular function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATP17	Q06405	1	EBI-2045988,EBI-3285

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 59	59	ATP synthase subunit J, mitochondrial		PRO_0000071697
Regions
	Transmembrane	9 – 25	17	Potential

Sequences

	Sequence		Length	Mass (Da)
	P81450-1 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: F0D7D450C4810025	FASTA	59	6,688
	10 20 30 40 50 MLKRFPTPIL KVYWPFFVAG AAVYYGMSKA ADLSSNTKEF INDPRNPRFA KGGKFVEVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of supernumerary yeast ATP synthase subunits e and i. Characterization of subunit i and disruption of its structural gene ATP18." Vaillier J., Arselin G., Graves P.-V., Camougrand N., Velours J. J. Biol. Chem. 274:543-548(1999) [PubMed: 9867878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15, IDENTIFICATION, MASS SPECTROMETRY. Strain: ATCC 24657 / D273-10B.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"ATP synthase of yeast mitochondria. Isolation of subunit j and disruption of the ATP18 gene." Arnold I., Pfeiffer K., Neupert W., Stuart R.A., Schaegger H. J. Biol. Chem. 274:36-40(1999) [PubMed: 9867807] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-13, IDENTIFICATION. Strain: ATCC 208353 / W303-1A.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF073791 Genomic DNA. Translation: AAD02949.1. Z46660 Genomic DNA. No translation available. AY558537 Genomic DNA. Translation: AAS56863.1.
PIR	S78730.
RefSeq	NP_013629.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3034N.
IntAct	P81450. 1 interaction.
STRING	P81450.
Genome annotation databases
Ensembl	YML081C-A; YML081C-A; YML081C-A; Saccharomyces cerevisiae. [Genome view]
GeneID	854893.
KEGG	sce:YML081C-A.
NMPDR	fig|4932.3.peg.4666.
Organism-specific databases
CYGD	YML081c-a.
SGD	S000007247. ATP18.
Phylogenomic databases
HOGENOM	P81450.
OMA	FINDPRH
OrthoDB	EOG9P2RJR
Gene expression databases
ArrayExpress	P81450.
Genevestigator	P81450.
GermOnline	YML081C-A. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006995. ATPase_F0-cplx_jsu. [Graphical view]
Pfam	PF04911. ATP-synt_J. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977855.

Entry information

Entry name

ATP18_YEAST

Accession

Primary (citable) accession number: P81450

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	November 24, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)