P81449 (ATPJ_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP synthase subunit e, mitochondrial
Short name=ATPase subunit e

Alternative name(s):
Translocase of the inner membrane protein 11

Gene names

Name:	TIM11
Synonyms:	ATP21
Ordered Locus Names:	YDR322C-A
ORF Names:	YDR322BC

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.
Subcellular location	Mitochondrion. Mitochondrion inner membrane.
Miscellaneous	Present with 4590 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion Mitochondrion inner membrane
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled proton transport Inferred from direct assay. Source: SGD cristae formation Inferred from mutant phenotype. Source: SGD protein oligomerization Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) Inferred from physical interaction Ref.5Ref.4. Source: SGD
Molecular function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 96	95	ATP synthase subunit e, mitochondrial		PRO_0000071689
Amino acid modifications
	Modified residue	2	1	N-acetylserine

Sequences

	Sequence		Length	Mass (Da)
	P81449 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 3A41A5F53EA75E98	FASTA	96	10,876
	10 20 30 40 50 60 MSTVNVLRYS ALGLGLFFGF RNDMILKCNA KKKEEQAQYE EKLKLVEEAK KEYAKLHPVV 70 80 90 TPKDVPANAS FNLEDPNIDF ERVILNAVES LKEAST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Yeast mitochondrial F1F0-ATPase exists as a dimer: identification of three dimer-specific subunits." Arnold I., Pfeiffer K., Neupert W., Stuart R.A., Schaegger H. EMBO J. 17:7170-7178(1998) [PubMed: 9857174] [Abstract] Cited for: IDENTIFICATION, PROTEIN SEQUENCE OF 2-14. Strain: ATCC 208353 / W303-1A.
[5]	"Yeast mitochondrial F1F0-ATPase: the novel subunit e is identical to Tim11." Arnold I., Bauer M.F., Brunner M., Neupert W., Stuart R.A. FEBS Lett. 411:195-200(1997) [PubMed: 9271204] [Abstract] Cited for: IDENTIFICATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32517 Genomic DNA. No translation available. AY557720 Genomic DNA. Translation: AAS56046.1. BK006938 Genomic DNA. Translation: DAA12165.1.
PIR	S78713.
RefSeq	NP_010609.1. NM_001184348.1.
3D structure databases
ProteinModelPortal	P81449.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3029N.
IntAct	P81449. 1 interaction.
MINT	MINT-2732377.
STRING	P81449.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR322C-A; YDR322C-A; YDR322C-A.
GeneID	851922.
KEGG	sce:YDR322C-A.
NMPDR	fig|4932.3.peg.1375.
Organism-specific databases
SGD	S000007255. TIM11.
Phylogenomic databases
eggNOG	fuNOG12720.
HOGENOM	HBG328835.
OMA	GSINWED.
OrthoDB	EOG466ZWQ.
Gene expression databases
ArrayExpress	P81449.
Genevestigator	P81449.
GermOnline	YDR322C-A. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008386. ATPase_F0-cplx_esu_mt. [Graphical view]
Pfam	PF05680. ATP-synt_E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969968.

Entry information

Entry name

ATPJ_YEAST

Accession

Primary (citable) accession number: P81449
Secondary accession number(s): D6VSV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program